دورية أكاديمية
أعرض في EDS

Transcriptional Expression of the ompA , cpaf , tarp, and tox Genes of Chlamydia trachomatis Clinical Isolates at Different Stages of the Developmental Cycle.

التفاصيل البيبلوغرافية
العنوان: Transcriptional Expression of the ompA , cpaf , tarp, and tox Genes of Chlamydia trachomatis Clinical Isolates at Different Stages of the Developmental Cycle.
المؤلفون: Korhonen S; Department of Virology, University of Helsinki and Helsinki University Hospital, Helsinki FI-00014, P.O. Box 21, Finland. suvi.j.korhonen@helsinki.fi., Hokynar K; Department of Virology, University of Helsinki and Helsinki University Hospital, Helsinki FI-00014, P.O. Box 21, Finland. kati.hokynar@helsinki.fi., Mannonen L; Department of Virology, University of Helsinki and Helsinki University Hospital, Helsinki FI-00014, P.O. Box 21, Finland. laura.mannonen@hus.fi., Paavonen J; Department of Obstetrics and Gynecology, University of Helsinki and Helsinki University Hospital, Helsinki FI-00029 HUS, P.O. Box 140, Finland. jorma.paavonen@helsinki.fi., Hiltunen-Back E; Clinic of Venereal Diseases, Skin and Allergy Hospital, Helsinki University Hospital, Helsinki FI-00029 HUS, P.O. Box 160, Finland. eija.hiltunen-back@hus.fi., Puolakkainen M; Department of Virology, University of Helsinki and Helsinki University Hospital, Helsinki FI-00014, P.O. Box 21, Finland. mirja.puolakkainen@helsinki.fi.
المصدر: Microorganisms [Microorganisms] 2019 May 28; Vol. 7 (6). Date of Electronic Publication: 2019 May 28.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101625893 Publication Model: Electronic Cited Medium: Print ISSN: 2076-2607 (Print) Linking ISSN: 20762607 NLM ISO Abbreviation: Microorganisms Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI AG, [2013]-
مستخلص: The transcriptional gene expression patterns of Chlamydia trachomatis have mainly been studied using reference strains propagated in cultured cells. Here, using five low-passage-number C. trachomatis clinical isolates that originated from asymptomatic or symptomatic female patients, the in vitro expression of the ompA , cpaf , tarp , and tox genes was studied with reverse transcriptase real-time PCR during the chlamydial developmental cycle. We observed dissimilarities in the gene expression patterns between the low-passage-number clinical isolates and the reference strains. The expression of ompA and the peak of the tox expression were observed earlier in the reference strains than in most of the clinical isolates. The expression of cpaf was high in the reference strains compared with the clinical isolates at the mid-phase (6-24 hours post infection) of the developmental cycle. All of the strains had a rather similar tarp expression profile. Four out of five clinical isolates exhibited slower growth kinetics compared with the reference strains. The use of low-passage-number C. trachomatis clinical isolates instead of reference strains in the studies might better reflect the situation in human infection.
Competing Interests: The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.
References: Mol Microbiol. 2000 Aug;37(4):913-25. (PMID: 10972811)
J Exp Med. 2001 Apr 16;193(8):935-42. (PMID: 11304554)
Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):13984-9. (PMID: 11707582)
J Bacteriol. 2003 May;185(10):3179-89. (PMID: 12730178)
Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8478-83. (PMID: 12815105)
Proc Natl Acad Sci U S A. 2003 Dec 23;100(26):15971-6. (PMID: 14673075)
Proc Natl Acad Sci U S A. 2004 Jul 6;101(27):10166-71. (PMID: 15199184)
Infect Immun. 2004 Dec;72(12):7063-72. (PMID: 15557630)
Microbes Infect. 2005 Mar;7(3):410-20. (PMID: 15784185)
J Clin Microbiol. 2006 Jan;44(1):206-13. (PMID: 16390971)
J Infect Dis. 2006 Aug 1;194(3):350-7. (PMID: 16826483)
Mol Microbiol. 2006 Dec;62(5):1387-401. (PMID: 17059564)
J Clin Microbiol. 2007 Aug;45(8):2649-53. (PMID: 17567790)
PLoS One. 2007 Sep 12;2(9):e878. (PMID: 17849007)
Gynecol Obstet Invest. 2009;68(3):160-6. (PMID: 19641324)
FEMS Immunol Med Microbiol. 2010 Mar;58(2):244-53. (PMID: 20002746)
PLoS One. 2010 Mar 25;5(3):e9887. (PMID: 20360858)
J Microbiol Methods. 2010 Sep;82(3):256-64. (PMID: 20619305)
Microbes Infect. 2011 Sep;13(10):824-7. (PMID: 21612765)
Infect Dis Obstet Gynecol. 2011;2011:481890. (PMID: 21747641)
PLoS Pathog. 2012;8(8):e1002842. (PMID: 22876181)
Infect Genet Evol. 2013 Jul;17:23-32. (PMID: 23542454)
Am J Med Sci. 2013 Jul;346(1):22-5. (PMID: 23792903)
Pathog Dis. 2014 Aug;71(3):336-51. (PMID: 24838663)
Toxins (Basel). 2015 Feb 17;7(2):621-37. (PMID: 25690695)
Pathog Dis. 2015 Aug;73(6):ftv043. (PMID: 26109550)
PLoS One. 2015 Jul 24;10(7):e0133420. (PMID: 26207372)
PLoS One. 2015 Dec 08;10(12):e0143304. (PMID: 26646541)
Nat Rev Microbiol. 2016 Jun;14(6):385-400. (PMID: 27108705)
Curr Opin Microbiol. 2017 Feb;35:78-87. (PMID: 28214646)
Microb Cell. 2016 Sep 05;3(9):390-403. (PMID: 28357377)
Genome Res. 2017 Jul;27(7):1220-1229. (PMID: 28588068)
Heliyon. 2017 Jul 26;3(7):e00364. (PMID: 28795162)
J Clin Microbiol. 1998 Feb;36(2):345-51. (PMID: 9466739)
معلومات مُعتمدة: 1285975 Academy of Finland; TYH2013405/MP, TYH2014117/MP, TYH2015318/MP Helsingin ja Uudenmaan Sairaanhoitopiiri
فهرسة مساهمة: Keywords: Chlamydia trachomatis; low-passage-number isolates; transcriptional expression
تواريخ الأحداث: Date Created: 20190531 Latest Revision: 20201001
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC6617244
DOI: 10.3390/microorganisms7060153
PMID: 31141911
قاعدة البيانات: MEDLINE
الوصف
تدمد:2076-2607
DOI:10.3390/microorganisms7060153