دورية أكاديمية
Transcriptional Expression of the ompA , cpaf , tarp, and tox Genes of Chlamydia trachomatis Clinical Isolates at Different Stages of the Developmental Cycle.
العنوان: | Transcriptional Expression of the ompA , cpaf , tarp, and tox Genes of Chlamydia trachomatis Clinical Isolates at Different Stages of the Developmental Cycle. |
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المؤلفون: | Korhonen S; Department of Virology, University of Helsinki and Helsinki University Hospital, Helsinki FI-00014, P.O. Box 21, Finland. suvi.j.korhonen@helsinki.fi., Hokynar K; Department of Virology, University of Helsinki and Helsinki University Hospital, Helsinki FI-00014, P.O. Box 21, Finland. kati.hokynar@helsinki.fi., Mannonen L; Department of Virology, University of Helsinki and Helsinki University Hospital, Helsinki FI-00014, P.O. Box 21, Finland. laura.mannonen@hus.fi., Paavonen J; Department of Obstetrics and Gynecology, University of Helsinki and Helsinki University Hospital, Helsinki FI-00029 HUS, P.O. Box 140, Finland. jorma.paavonen@helsinki.fi., Hiltunen-Back E; Clinic of Venereal Diseases, Skin and Allergy Hospital, Helsinki University Hospital, Helsinki FI-00029 HUS, P.O. Box 160, Finland. eija.hiltunen-back@hus.fi., Puolakkainen M; Department of Virology, University of Helsinki and Helsinki University Hospital, Helsinki FI-00014, P.O. Box 21, Finland. mirja.puolakkainen@helsinki.fi. |
المصدر: | Microorganisms [Microorganisms] 2019 May 28; Vol. 7 (6). Date of Electronic Publication: 2019 May 28. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101625893 Publication Model: Electronic Cited Medium: Print ISSN: 2076-2607 (Print) Linking ISSN: 20762607 NLM ISO Abbreviation: Microorganisms Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI AG, [2013]- |
مستخلص: | The transcriptional gene expression patterns of Chlamydia trachomatis have mainly been studied using reference strains propagated in cultured cells. Here, using five low-passage-number C. trachomatis clinical isolates that originated from asymptomatic or symptomatic female patients, the in vitro expression of the ompA , cpaf , tarp , and tox genes was studied with reverse transcriptase real-time PCR during the chlamydial developmental cycle. We observed dissimilarities in the gene expression patterns between the low-passage-number clinical isolates and the reference strains. The expression of ompA and the peak of the tox expression were observed earlier in the reference strains than in most of the clinical isolates. The expression of cpaf was high in the reference strains compared with the clinical isolates at the mid-phase (6-24 hours post infection) of the developmental cycle. All of the strains had a rather similar tarp expression profile. Four out of five clinical isolates exhibited slower growth kinetics compared with the reference strains. The use of low-passage-number C. trachomatis clinical isolates instead of reference strains in the studies might better reflect the situation in human infection. Competing Interests: The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. |
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معلومات مُعتمدة: | 1285975 Academy of Finland; TYH2013405/MP, TYH2014117/MP, TYH2015318/MP Helsingin ja Uudenmaan Sairaanhoitopiiri |
فهرسة مساهمة: | Keywords: Chlamydia trachomatis; low-passage-number isolates; transcriptional expression |
تواريخ الأحداث: | Date Created: 20190531 Latest Revision: 20201001 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC6617244 |
DOI: | 10.3390/microorganisms7060153 |
PMID: | 31141911 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2076-2607 |
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DOI: | 10.3390/microorganisms7060153 |