دورية أكاديمية
أعرض في EDS

Concordance of the point-of-care circulating cathodic antigen test for the diagnosis of intestinal schistosomiasis in a low endemicity area.

التفاصيل البيبلوغرافية
العنوان: Concordance of the point-of-care circulating cathodic antigen test for the diagnosis of intestinal schistosomiasis in a low endemicity area.
المؤلفون: de Sousa SRM; Programa de Pós-Graduação Strictu Sensu em Biologia Parasitária na Amazônia da Universidade do Estado do Pará, Tv. Perebebuí, 2623 - Marco, Belém, PA, 66087, Brazil. rodrigo.bio.uepa@gmail.com.; Laboratório de Parasitoses Intestinais, Esquistossomose e Malacologia (LPIEM), Secção de Parasitologia, Instituto Evandro Chagas/SVS/MS, Ananindeua, Pará, Brazil. rodrigo.bio.uepa@gmail.com., Dias IHL; Programa de Pós-Graduação Strictu Sensu em Biologia Parasitária na Amazônia da Universidade do Estado do Pará, Tv. Perebebuí, 2623 - Marco, Belém, PA, 66087, Brazil.; Laboratório de Parasitoses Intestinais, Esquistossomose e Malacologia (LPIEM), Secção de Parasitologia, Instituto Evandro Chagas/SVS/MS, Ananindeua, Pará, Brazil., Fonseca ÁLS; Programa de Pós-Graduação Strictu Sensu em Biologia Parasitária na Amazônia da Universidade do Estado do Pará, Tv. Perebebuí, 2623 - Marco, Belém, PA, 66087, Brazil.; Laboratório de Parasitoses Intestinais, Esquistossomose e Malacologia (LPIEM), Secção de Parasitologia, Instituto Evandro Chagas/SVS/MS, Ananindeua, Pará, Brazil., Contente BR; Programa de Pós-Graduação Strictu Sensu em Biologia Parasitária na Amazônia da Universidade do Estado do Pará, Tv. Perebebuí, 2623 - Marco, Belém, PA, 66087, Brazil.; Laboratório de Parasitoses Intestinais, Esquistossomose e Malacologia (LPIEM), Secção de Parasitologia, Instituto Evandro Chagas/SVS/MS, Ananindeua, Pará, Brazil., Nogueira JFC; Programa de Pós-Graduação Strictu Sensu em Biologia Parasitária na Amazônia da Universidade do Estado do Pará, Tv. Perebebuí, 2623 - Marco, Belém, PA, 66087, Brazil.; Laboratório de Parasitoses Intestinais, Esquistossomose e Malacologia (LPIEM), Secção de Parasitologia, Instituto Evandro Chagas/SVS/MS, Ananindeua, Pará, Brazil., da Costa Oliveira TN; Programa de Pós-Graduação Strictu Sensu em Biologia Parasitária na Amazônia da Universidade do Estado do Pará, Tv. Perebebuí, 2623 - Marco, Belém, PA, 66087, Brazil., Geiger SM; Departamento de Parasitologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Enk MJ; Programa de Pós-Graduação Strictu Sensu em Biologia Parasitária na Amazônia da Universidade do Estado do Pará, Tv. Perebebuí, 2623 - Marco, Belém, PA, 66087, Brazil.; Laboratório de Parasitoses Intestinais, Esquistossomose e Malacologia (LPIEM), Secção de Parasitologia, Instituto Evandro Chagas/SVS/MS, Ananindeua, Pará, Brazil.
المصدر: Infectious diseases of poverty [Infect Dis Poverty] 2019 May 30; Vol. 8 (1), pp. 37. Date of Electronic Publication: 2019 May 30.
نوع المنشور: Comparative Study; Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101606645 Publication Model: Electronic Cited Medium: Internet ISSN: 2049-9957 (Electronic) Linking ISSN: 20499957 NLM ISO Abbreviation: Infect Dis Poverty Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, 2012-
مواضيع طبية MeSH: Antigens, Helminth/*analysis , Immunoassay/*methods , Schistosoma mansoni/*immunology , Schistosomiasis mansoni/*diagnosis, Adolescent ; Adult ; Aged ; Animals ; Brazil/epidemiology ; Child ; Feces/parasitology ; Female ; Humans ; Male ; Middle Aged ; Point-of-Care Systems ; Schistosoma mansoni/isolation & purification ; Schistosomiasis mansoni/epidemiology ; Sensitivity and Specificity ; Urine/parasitology ; Young Adult
مستخلص: Background: The Kato-Katz technique is recommended worldwide for the diagnosis of intestinal schistosomiasis, detecting parasite eggs in feces of infected people. However, new tests have been developed in order to facilitate diagnosis, e.g. by detection of specific antigens secreted by schistosomes, such as the circulating cathodic antigen (CCA). The aim of this study was to evaluate the performance of the point-of-care circulating cathodic antigen test (POC-CCA) compared to the Kato-Katz technique in a low prevalence area in the Amazon Region, located in the municipality of Primavera, State of Pará, Brazil.
Methods: Positivity rates of the POC-CCA test and the Kato-Katz technique were calculated. The sensitivity, specificity, accuracy and kappa coefficient were determined by comparing both methods. The reference standard was established using 16 Kato-Katz slides, 12 of the first fecal sample, two of the second and two of the third one. The study also included the concordance between POC-CCA results and different numbers and combinations of Kato-Katz slides.
Results: The prevalence of schistosomiasis according to the reference standard or POC-CCA test reached a rate of 9.4% or 23.9%, respectively, among a total of 372 participants. The positivity rates by the Kato-Katz technique increased from 2.4 to 9.4%, according to the increase in the number of slides examined and fecal samples collected. A sensitivity of 55.6%, specificity 76.9%, accuracy 76% and κ coefficient of 0.06 was observed by comparing one slide of the first sample and POC-CCA. Comparing 6 slides from three different samples, two slides of each, with POC-CCA resulted in a sensitivity of 58.3%, specificity 78.4%, accuracy 77% and κ coefficient of 0.16. Finally, the comparison of 16 slides from three different samples with POC-CCA revealed a sensitivity of 65.7%, specificity 80.4%, accuracy 79%, and κ coefficient of 0.27.
Conclusions: The immunochromatographic test has the potential to be an important tool to combat schistosomiasis because of its practicality and applicability but should be applied with caution in low prevalence areas and in programs that aim to eliminate this disease.
Trial Registration: CAAE#21824513.9.0000.5091 . January 31st, 2014.
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معلومات مُعتمدة: 0042017 Fundação de Amparo à Pesquisa do Estado do Pará (BR); MCTI/CNPq/MS-SCTIE-Decit n 40/2012 Ministério da Ciência, Tecnologia e Inovação
فهرسة مساهمة: Keywords: Accuracy; Diagnosis; Kato-Katz; Low prevalence area; POC-CCA; Schistosoma mansoni
المشرفين على المادة: 0 (Antigens, Helminth)
تواريخ الأحداث: Date Created: 20190531 Date Completed: 20190625 Latest Revision: 20200225
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC6542115
DOI: 10.1186/s40249-019-0551-7
PMID: 31142379
قاعدة البيانات: MEDLINE
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