دورية أكاديمية
Cooperation between tropomyosin and α-actinin inhibits fimbrin association with actin filament networks in fission yeast.
| العنوان: | Cooperation between tropomyosin and α-actinin inhibits fimbrin association with actin filament networks in fission yeast. |
|---|---|
| المؤلفون: | Christensen JR; Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, United States., Homa KE; Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, United States., Morganthaler AN; Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, United States., Brown RR; Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, United States., Suarez C; Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, United States., Harker AJ; Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, United States., O'Connell ME; Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, United States., Kovar DR; Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, United States.; Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, United States. |
| المصدر: | ELife [Elife] 2019 Jun 10; Vol. 8. Date of Electronic Publication: 2019 Jun 10. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: eLife Sciences Publications, Ltd Country of Publication: England NLM ID: 101579614 Publication Model: Electronic Cited Medium: Internet ISSN: 2050-084X (Electronic) Linking ISSN: 2050084X NLM ISO Abbreviation: Elife Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge, UK : eLife Sciences Publications, Ltd., 2012- |
| مواضيع طبية MeSH: | Actin Cytoskeleton/*metabolism , Actinin/*metabolism , Membrane Glycoproteins/*metabolism , Microfilament Proteins/*metabolism , Schizosaccharomyces/*metabolism , Schizosaccharomyces pombe Proteins/*metabolism , Tropomyosin/*metabolism, Actin Depolymerizing Factors/genetics ; Actin Depolymerizing Factors/metabolism ; Actinin/genetics ; Actins/genetics ; Actins/metabolism ; Cell Cycle Proteins/genetics ; Cell Cycle Proteins/metabolism ; Green Fluorescent Proteins/genetics ; Green Fluorescent Proteins/metabolism ; Membrane Glycoproteins/genetics ; Microfilament Proteins/genetics ; Microscopy, Fluorescence/methods ; Protein Binding ; Schizosaccharomyces/genetics ; Schizosaccharomyces pombe Proteins/genetics ; Time-Lapse Imaging/methods ; Tropomyosin/genetics |
| مستخلص: | We previously discovered that competition between fission yeast actin binding proteins (ABPs) for binding F-actin facilitates their sorting to different cellular networks. Specifically, competition between endocytic actin patch ABPs fimbrin Fim1 and cofilin Adf1 enhances their activities, and prevents tropomyosin Cdc8's association with actin patches. However, these interactions do not explain how Fim1 is prevented from associating strongly with other F-actin networks such as the contractile ring. Here, we identified α-actinin Ain1, a contractile ring ABP, as another Fim1 competitor. Fim1 competes with Ain1 for association with F-actin, which is dependent upon their F-actin residence time. While Fim1 outcompetes both Ain1 and Cdc8 individually, Cdc8 enhances the F-actin bundling activity of Ain1, allowing Ain1 to generate F-actin bundles that Cdc8 can bind in the presence of Fim1. Therefore, the combination of contractile ring ABPs Ain1 and Cdc8 is capable of inhibiting Fim1's association with F-actin networks. Competing Interests: JC, KH, AM, RB, CS, AH, MO, DK No competing interests declared (© 2019, Christensen et al.) |
| References: | J Cell Biol. 1994 Jul;126(2):413-22. (PMID: 8034742) Curr Biol. 2010 Aug 24;20(16):1415-22. (PMID: 20705466) Mol Biol Cell. 2016 Jun 1;27(11):1821-33. (PMID: 27075176) J Biochem. 2017 Aug 1;162(2):93-102. (PMID: 28338873) J Biol Chem. 2011 Jul 29;286(30):26964-77. (PMID: 21642440) Proc Natl Acad Sci U S A. 2012 Feb 21;109(8):2913-8. (PMID: 22308368) PeerJ. 2016 Mar 28;4:e1858. (PMID: 27069798) Eur J Cell Biol. 1998 Aug;76(4):288-95. (PMID: 9765059) Nat Struct Mol Biol. 2010 May;17(5):614-6. (PMID: 20383143) Cell Rep. 2018 Jul 10;24(2):503-514. (PMID: 29996109) J Mol Biol. 1971 Nov 28;62(1):251-65. (PMID: 4945533) J Biol Chem. 1971 Aug 10;246(15):4866-71. (PMID: 4254541) J Cell Sci. 2007 May 1;120(Pt 9):1635-45. (PMID: 17452625) Science. 2005 Oct 14;310(5746):310-4. (PMID: 16224022) J Cell Biol. 2011 Mar 21;192(6):1005-21. (PMID: 21422229) Mol Biol Cell. 2001 Apr;12(4):1061-77. (PMID: 11294907) Trends Cell Biol. 2011 Mar;21(3):177-87. (PMID: 21145239) Structure. 2010 Jul 14;18(7):761-7. (PMID: 20637412) Nature. 2009 Aug 20;460(7258):1031-4. (PMID: 19648907) Curr Biol. 2014 Jul 7;24(13):1525-30. (PMID: 24954052) Nat Methods. 2012 Jun 28;9(7):676-82. (PMID: 22743772) Elife. 2017 Mar 10;6:. (PMID: 28282023) Methods Mol Biol. 2016;1369:151-79. (PMID: 26519312) Curr Biol. 2016 Oct 24;26(20):2697-2706. (PMID: 27666967) Mol Biol Cell. 2001 Nov;12(11):3515-26. (PMID: 11694585) Biol Open. 2015 Jul 17;4(8):1040-51. (PMID: 26187949) Curr Biol. 2011 Jul 26;21(14):R560-9. (PMID: 21783039) J Biol Chem. 1995 May 12;270(19):11437-44. (PMID: 7744781) J Cell Biol. 1994 Jul;126(2):423-32. (PMID: 8034743) Nat Commun. 2016 Apr 12;7:11265. (PMID: 27068241) Eur J Cell Biol. 2013 Oct-Nov;92(10-11):333-8. (PMID: 24252518) Mol Cell Proteomics. 2015 May;14(5):1275-87. (PMID: 25720772) J Cell Sci. 2010 Oct 1;123(Pt 19):3235-43. (PMID: 20807799) J Biol Chem. 2018 Sep 14;293(37):14520-14533. (PMID: 30049798) J Cell Biol. 1994 Jul;126(2):433-43. (PMID: 8034744) Mol Biol Cell. 2010 Aug 15;21(16):2894-904. (PMID: 20587778) Biophys J. 2006 Oct 1;91(7):2564-72. (PMID: 16829561) Curr Biol. 2014 Mar 3;24(5):579-85. (PMID: 24560576) Proc Natl Acad Sci U S A. 2008 Feb 5;105(5):1494-8. (PMID: 18234857) Dev Cell. 2003 Nov;5(5):723-34. (PMID: 14602073) Nat Methods. 2012 Jul;9(7):671-5. (PMID: 22930834) J Cell Biol. 2012 Nov 26;199(5):831-47. (PMID: 23185032) |
| معلومات مُعتمدة: | RSG-11-126-01-CSM International American Cancer Society; T32 GM0071832 United States NH NIH HHS; R01 GM079265 United States GM NIGMS NIH HHS; R01 GM071832 United States GM NIGMS NIH HHS; R25 GM109439 United States GM NIGMS NIH HHS; Graduate Research Fellowship DGE-1746045 International National Science Foundation; R01 GM079265 United States NH NIH HHS; IMSD R25GM109439 United States NH NIH HHS; Graduate Research Fellowship DGE-1144082 International National Science Foundation |
| فهرسة مساهمة: | Keywords: Ain1; Cdc8; S. pombe; actin patch; cell biology; contractile ring; cytokinesis; endocytosis |
| المشرفين على المادة: | 0 (Actin Depolymerizing Factors) 0 (Actins) 0 (Adf1 protein, S pombe) 0 (Cdc8 protein, S pombe) 0 (Cell Cycle Proteins) 0 (Membrane Glycoproteins) 0 (Microfilament Proteins) 0 (Schizosaccharomyces pombe Proteins) 0 (Tropomyosin) 0 (plastin) 11003-00-2 (Actinin) 147336-22-9 (Green Fluorescent Proteins) |
| تواريخ الأحداث: | Date Created: 20190611 Date Completed: 20200302 Latest Revision: 20200309 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC6557641 |
| DOI: | 10.7554/eLife.47279 |
| PMID: | 31180322 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder كن أول من يترك تعليقا!