دورية أكاديمية
Entomo-virological surveillance strategy for dengue, Zika and chikungunya arboviruses in field-caught Aedes mosquitoes in an endemic urban area of the Northeast of Brazil.
| العنوان: | Entomo-virological surveillance strategy for dengue, Zika and chikungunya arboviruses in field-caught Aedes mosquitoes in an endemic urban area of the Northeast of Brazil. |
|---|---|
| المؤلفون: | Dos Reis IC; Laboratório de Mosquitos Transmissores de Hematozóarios- LATHEMA, Fundação Oswaldo Cruz - Fiocruz, Rio de Janeiro, RJ, Brazil; Núcleo Operacional Sentinela de Mosquitos Vetores - Nosmove, Fundação Oswaldo Cruz- Fiocruz, Rio de Janeiro, RJ, Brazil., Gibson G; Instituto de Estudos em Saúde Coletiva- IESC, Universidade Federal do Rio de Janeiro - UFRJ, Brazil. Electronic address: gibson.gerusa@iesc.ufrj.br., Ayllón T; Núcleo Operacional Sentinela de Mosquitos Vetores - Nosmove, Fundação Oswaldo Cruz- Fiocruz, Rio de Janeiro, RJ, Brazil., de Medeiros Tavares A; Centro de Controle de Zoonoses, Secretaria Municipal de Saúde de Natal - SMS, Brazil., de Araújo JMG; Laboratório de Biologia Molecular de Doenças Infecciosas e do Câncer, Universidade Federal do Rio Grande do Norte - UFRN, Brazil; Laboratório de Virologia, Instituto de Medicina Tropical, Universidade Federal do Rio Grande do Norte- UFRN, Brazil., da Silva Monteiro E; Centro de Controle de Zoonoses, Secretaria Municipal de Saúde de Natal - SMS, Brazil., Rodrigues Aguiar A; Centro de Controle de Zoonoses, Secretaria Municipal de Saúde de Natal - SMS, Brazil., de Oliveira JV; Centro de Controle de Zoonoses, Secretaria Municipal de Saúde de Natal - SMS, Brazil., de Paiva AAP; Laboratório de Biologia Molecular de Doenças Infecciosas e do Câncer, Universidade Federal do Rio Grande do Norte - UFRN, Brazil., Wana Bezerra Pereira H; Laboratório de Biologia Molecular de Doenças Infecciosas e do Câncer, Universidade Federal do Rio Grande do Norte - UFRN, Brazil., Dantas Monteiro J; Laboratório de Biologia Molecular de Doenças Infecciosas e do Câncer, Universidade Federal do Rio Grande do Norte - UFRN, Brazil; Laboratório de Virologia, Instituto de Medicina Tropical, Universidade Federal do Rio Grande do Norte- UFRN, Brazil., Sá Carvalho M; Programa de Computação Científica (PROCC), Fundação Oswaldo Cruz - Fiocruz, Rio de Janeiro, RJ, Brazil., Sabroza PC; Escola Nacional de Saúde Pública (ENSP), Fundação Oswaldo Cruz - Fiocruz, Rio de Janeiro, RJ, Brazil., Alves Honório N; Laboratório de Mosquitos Transmissores de Hematozóarios- LATHEMA, Fundação Oswaldo Cruz - Fiocruz, Rio de Janeiro, RJ, Brazil; Núcleo Operacional Sentinela de Mosquitos Vetores - Nosmove, Fundação Oswaldo Cruz- Fiocruz, Rio de Janeiro, RJ, Brazil. Electronic address: honorio@ioc.fiocruz.br. |
| مؤلفون مشاركون: | ARBOALVO Network |
| المصدر: | Acta tropica [Acta Trop] 2019 Sep; Vol. 197, pp. 105061. Date of Electronic Publication: 2019 Jun 10. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0370374 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-6254 (Electronic) Linking ISSN: 0001706X NLM ISO Abbreviation: Acta Trop Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Basel. |
| مواضيع طبية MeSH: | Aedes/*virology , Chikungunya virus/*isolation & purification , Dengue Virus/*isolation & purification , Zika Virus/*isolation & purification, Adult ; Animals ; Endemic Diseases/statistics & numerical data ; Female ; Humans ; Male ; Mosquito Vectors/virology |
| مستخلص: | Aedes spp. are considered the main vectors of dengue (DENV), Zika (ZIKV) and chikungunya (CHIKV) viruses in the world. Arbovirus detection in Aedes mosquitoes can alert authorities to possible outbreaks, reducing the impact of these diseases. The purpose of this study was to perform an operational strategy for virological surveillance of DENV, ZIKV and CHIKV in adult Aedes aegypti and Aedes albopictus mosquitoes captured at different key-sites in an endemic urban area of the Northeast Region of Brazil, with the prospect of discussing its role as part of an alert system for outbreaks in critical areas. Residential and non-residential premises located in areas of recent of transmission of these arboviruses were selected for adult mosquito collection in the rainy season (July) of 2018. A total of 1068 adult mosquitoes were collected: 946 Culex quinquefasciatus (88.6%), 118 Ae. aegypti (11.0%), two Ae. albopictus (0.2%) and two Aedes taeniorhynchus (0.2%). Among the premises surveyed, recycling points (N = 48, 40.7%), municipal schools (N = 36, 30.5%) and junkyards (N = 31, 26.2%) were the places with the highest frequency of adult Ae. aegypti. Health units (including primary health care facilities and one hospital) (N = 23; 19.5%) together with residential premises (N = 11; 9.3%) presented the lowest frequencies. Total RNAs of the samples were extracted from Aedes mosquitoes and a nested reverse transcription (RT) polymerase chain reaction (PCR) assay for detecting and typing DENV, ZIKV and CHIKV was performed. From the 37 Aedes spp. pools analyzed (35 Ae. aegypti, one Ae. albopictus and one Ae. taeniorhynchus), seven were positive for DENV-3, including three pools containing Ae. aegypti females, one containing an Ae. aegypti engorged female and three comprised of Ae. aegypti males. The positive pools were composed of mosquitoes collected in public schools, health units, junkyards, recycling points and residential premises. Our findings reinforce the importance of continuous virological surveillance in Aedes mosquitoes, as a useful tool for detecting arboviruses circulation in vulnerable areas, even in low infestation seasons. (Copyright © 2019 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Aedes spp.; Arboviruses; DENV-3; Entomo-virological surveillance; Key-sites |
| تواريخ الأحداث: | Date Created: 20190614 Date Completed: 20191122 Latest Revision: 20191122 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.actatropica.2019.105061 |
| PMID: | 31194961 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder كن أول من يترك تعليقا!