دورية أكاديمية
Gene silencing by RNAi via oral delivery of dsRNA by bacteria in the South American tomato pinworm, Tuta absoluta.
| العنوان: | Gene silencing by RNAi via oral delivery of dsRNA by bacteria in the South American tomato pinworm, Tuta absoluta. |
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| المؤلفون: | Bento FM; Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba, Brazil.; Escola Superior de Agricultura 'Luiz de Queiroz' (ESALQ), Universidade de São Paulo, Piracicaba, Brazil., Marques RN; Centro de Ciências Agrárias, Universidade Federal de São Carlos, Araras, Brazil., Campana FB; Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba, Brazil., Demétrio CG; Escola Superior de Agricultura 'Luiz de Queiroz' (ESALQ), Universidade de São Paulo, Piracicaba, Brazil., Leandro RA; Escola Superior de Agricultura 'Luiz de Queiroz' (ESALQ), Universidade de São Paulo, Piracicaba, Brazil., Parra JRP; Escola Superior de Agricultura 'Luiz de Queiroz' (ESALQ), Universidade de São Paulo, Piracicaba, Brazil., Figueira A; Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba, Brazil. |
| المصدر: | Pest management science [Pest Manag Sci] 2020 Jan; Vol. 76 (1), pp. 287-295. Date of Electronic Publication: 2019 Jul 16. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Published for SCI by Wiley Country of Publication: England NLM ID: 100898744 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1526-4998 (Electronic) Linking ISSN: 1526498X NLM ISO Abbreviation: Pest Manag Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: West Sussex, UK : Published for SCI by Wiley, c2000- |
| مواضيع طبية MeSH: | Enterobius* , Solanum lycopersicum*, Animals ; Gene Silencing ; RNA Interference ; RNA, Double-Stranded |
| مستخلص: | Background: RNA interference (RNAi) has been evaluated in several insect pests as a novel strategy to be included in integrated pest management. Lepidopterans are recognized to be recalcitrant to gene silencing by RNAi. As such, double-stranded RNA (dsRNA) delivery needs to be adjusted to assure its stability until it reaches the target gene transcript for silencing. Gene silencing by RNAi offers the potential to be used in the control of Tuta absoluta (Meyrick), one of the main insect pests of tomato (Solanum lycopersicum) worldwide. Here, we tested the delivery of dsRNA expressed in Escherichia coli HT115(DE3) and supplied to larvae in an artificial diet by screening target genes for silencing. We tested six target genes: juvenile hormone inducible protein (JHP); juvenile hormone epoxide hydrolase protein (JHEH); ecdysteroid 25-hydroxylase (PHM); chitin synthase A (CHI); carboxylesterase (COE); and arginine kinase (AK). Results: Based on larval mortality, the duration of the larval stage in days, pupal weight, and the accumulation of the target gene transcript, we demonstrated the efficacy of bacterial dsRNA delivery for the functional effects on larval development. Providing dsRNA targeted to JHP, CHI, COE and AK by bacteria led to a significant decrease in transcript accumulation and an increase in larval mortality. Conclusion: Bacteria expressing dsRNA targeting essential T. absoluta genes supplied in artificial diet are efficient to screen RNAi target-genes. The oral delivery of dsRNA by bacteria is a novel potential alternative for the control of T. absoluta based on RNAi. © 2019 Society of Chemical Industry. (© 2019 Society of Chemical Industry.) |
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| معلومات مُعتمدة: | 141030/2015-1 Conselho Nacional de Desenvolvimento Científico e Tecnológico; 2011/12869-6 Fundação de Amparo à Pesquisa do Estado de São Paulo |
| فهرسة مساهمة: | Keywords: Escherichia coli HT115(DE3); Solanum lycopersicum; bacteria dsRNA delivery; reference genes |
| المشرفين على المادة: | 0 (RNA, Double-Stranded) |
| تواريخ الأحداث: | Date Created: 20190618 Date Completed: 20200316 Latest Revision: 20221207 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1002/ps.5513 |
| PMID: | 31207074 |
| قاعدة البيانات: | MEDLINE |
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