دورية أكاديمية
Dual effective core-shell electrospun scaffolds: Promoting osteoblast maturation and reducing bacteria activity.
| العنوان: | Dual effective core-shell electrospun scaffolds: Promoting osteoblast maturation and reducing bacteria activity. |
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| المؤلفون: | De-Paula MMM; University of Vale do Paraiba, 12244-000 Sao Jose dos Campos, SP, Brazil., Afewerki S; Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA; Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA., Viana BC; Department of Physics and Materials Science and Engineering graduate program, Federal University of Piaui, 64049-550 Teresina, PI, Brazil., Webster TJ; Nanomedicine Laboratory, Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USA. Electronic address: th.webster@neu.edu., Lobo AO; Interdisciplinary Laboratory for Advanced Materials, Department of Materials Engineering, Federal University of Piaui, 64049-550 Teresina, PI, Brazil. Electronic address: lobo@ufpi.edu.br., Marciano FR; Department of Physics and Materials Science and Engineering graduate program, Federal University of Piaui, 64049-550 Teresina, PI, Brazil. Electronic address: femarciano@gmail.com. |
| المصدر: | Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2019 Oct; Vol. 103, pp. 109778. Date of Electronic Publication: 2019 May 21. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101484109 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-0191 (Electronic) Linking ISSN: 09284931 NLM ISO Abbreviation: Mater Sci Eng C Mater Biol Appl Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam : Elsevier |
| مواضيع طبية MeSH: | Histones*/chemistry , Histones*/pharmacology , Intercellular Signaling Peptides and Proteins*/chemistry , Intercellular Signaling Peptides and Proteins*/pharmacology , Tissue Engineering*, Cell Differentiation/*drug effects , Methicillin-Resistant Staphylococcus aureus/*growth & development , Osteoblasts/*metabolism , Pseudomonas aeruginosa/*growth & development , Tissue Scaffolds/*chemistry, Cell Line ; Humans |
| مستخلص: | Herein, we electrospun ultrathin core-shell fibers based on polycaprolactone (PCL), polyethylene glycol (PEG), gelatin and osteogenic growth peptide (OGP), and evaluated their potential to upregulate human osteoblast cells (hFOB) and to reduce Gram-positive and Gram-negative bacteria. We also evaluated the fiber morphology, chemical structure and peptide delivery efficacy. The employment of core-shell fibers compared to fibers without a core-shell showed improved mechanical strength, comparable to the strength of pure PCL, as well as improved hydrophilicity and wettability. The careful selection of polymer combination and core-shell strategy promoted a controlled and sustained release of OGP. Moreover, increased calcium deposition (CD) (1.3-fold) and alkaline phosphate (ALP) activity was observed when hFOBs were cultivated onto core-shell fibers loaded with OGP after 21 days of culture. Our developed scaffolds were also able to reduce the amount of Pseudomonas aeruginosa (ATCC 25668) bacteria by a factor of two compared to raw PCL without the use of any antibiotics. All of these results demonstrate a promising potential of the developed core-shell electrospun scaffolds based on PCL:PEG:Gelatin:OGP for numerous bone tissue applications. (Copyright © 2019 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Bactericidal effect; Bone regeneration; Core-shell; Electrospinning; Osteogenic growth peptide; PCL |
| المشرفين على المادة: | 0 (Histones) 0 (Intercellular Signaling Peptides and Proteins) 132996-61-3 (osteogenic growth peptide) |
| تواريخ الأحداث: | Date Created: 20190728 Date Completed: 20191230 Latest Revision: 20191230 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.msec.2019.109778 |
| PMID: | 31349506 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-0191 |
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| DOI: | 10.1016/j.msec.2019.109778 |