دورية أكاديمية
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Prenylated flavonoid-enriched fraction from Maclura tinctoria shows biological activity against Staphylococcus aureus and protects Galleria mellonella larvae from bacterial infection.

التفاصيل البيبلوغرافية
العنوان: Prenylated flavonoid-enriched fraction from Maclura tinctoria shows biological activity against Staphylococcus aureus and protects Galleria mellonella larvae from bacterial infection.
المؤلفون: das Chagas Almeida A; Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil., Azevedo Rodrigues L; Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil., Dos Santos Paulino G; Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil., Pereira Aguilar A; Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil., Andrade Almeida A; Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil., Olavo Ferreira S; Departamento de Física, Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil., Brandão GC; Escola de Farmácia, Departamento de Farmácia, Universidade Federal de Ouro Preto, Ouro Preto, MG, 35400-000, Brazil., Viana Leite JP; Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil., de Oliveira Barros Ribon A; Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Viçosa, MG, 36570-900, Brazil. abribon@ufv.br.
المصدر: BMC complementary and alternative medicine [BMC Complement Altern Med] 2019 Jul 29; Vol. 19 (1), pp. 189. Date of Electronic Publication: 2019 Jul 29.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101088661 Publication Model: Electronic Cited Medium: Internet ISSN: 1472-6882 (Electronic) Linking ISSN: 14726882 NLM ISO Abbreviation: BMC Complement Altern Med Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2001-2019]
مواضيع طبية MeSH: Anti-Bacterial Agents/*administration & dosage , Flavonoids/*administration & dosage , Maclura/*chemistry , Moths/*microbiology , Plant Extracts/*administration & dosage , Protective Agents/*administration & dosage , Staphylococcal Infections/*prevention & control , Staphylococcus aureus/*drug effects, Animals ; Anti-Bacterial Agents/chemistry ; Brazil ; Disease Models, Animal ; Flavonoids/chemistry ; Humans ; Larva/growth & development ; Larva/microbiology ; Microbial Sensitivity Tests ; Moths/growth & development ; Plant Extracts/chemistry ; Protective Agents/chemistry ; Staphylococcal Infections/microbiology
مستخلص: Background: The Atlantic Forest biome extends along the entire Brazilian coast and is home to approximately 20,000 plant species, many of which are endemic; it is considered one of the hotspot regions of the planet. Several of these species are sources of natural products with biological activities that are still unknown. In this study, we evaluated the antimicrobial activity of 90 extracts derived from native Atlantic Forest tree species against Staphylococcus aureus, an important human and veterinary pathogen.
Methods: Extracts from native Atlantic Forest tree species were evaluated for their antimicrobial activity against S. aureus by in vitro standard methods. Phytochemical fractionation of the extract from Maclura tinctoria was performed by liquid-liquid partitioning. LC-DAD-ESI-MS was used for identification of constituents in the most active fraction. Damage of cells and alterations in the permeability of cell membrane were determined by atomic force microscopy (AFM) and crystal violet uptake assay, respectively. In vivo antimicrobial activity was evaluated using Galleria mellonella larvae infected with S. aureus with survival data collected using the Kaplan-Meier method.
Results: Among the organic or aqueous extracts tested here, 26 showed biological activity. Eight species showed relevant results, with a minimum inhibitory concentration (MIC) below 1 mg/mL. Antibacterial activity was registered for three species for the first time. An organic extract from Maclura tinctoria leaves showed the lowest MIC (0.08 mg/mL). Fractionation of this extract by liquid-liquid partitioning led to obtaining fraction 11FO d with a MIC of 0.04 mg/mL. This fraction showed strong activity against veterinary S. aureus isolates and contributed to the increased survival of Galleria mellonella larvae infected with S. aureus ATCC 29213. The bacterial surface was not altered by the presence of 11FO d, and no cell membrane damage was detected. The LC-DAD-ESI/MS analyses identified prenylated flavonoids as the major constituents responsible for the antibacterial activity of this active extract.
Conclusion: A fraction enriched in prenylated isoflavones and flavanones from M. tinctoria showed in vitro and in vivo efficacy as antistaphylococcal agents. These findings justify the need for further research to elucidate the mechanisms of action of these compounds.
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فهرسة مساهمة: Keywords: Antibacterial; Galleria mellonella; Maclura tinctoria; Prenylated flavonoids
المشرفين على المادة: 0 (Anti-Bacterial Agents)
0 (Flavonoids)
0 (Plant Extracts)
0 (Protective Agents)
تواريخ الأحداث: Date Created: 20190731 Date Completed: 20191216 Latest Revision: 20200225
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC6664575
DOI: 10.1186/s12906-019-2600-y
PMID: 31357964
قاعدة البيانات: MEDLINE
الوصف
تدمد:1472-6882
DOI:10.1186/s12906-019-2600-y