دورية أكاديمية
Bicistronic Construct for Optogenetic Prosthesis of Ganglion Cell Receptive Field of Degenerative Retina.
| العنوان: | Bicistronic Construct for Optogenetic Prosthesis of Ganglion Cell Receptive Field of Degenerative Retina. |
|---|---|
| المؤلفون: | Petrovskaya LE; Pirogov Russian National Research Medical University, Ministry of Healthcare of the Russian Federation, 117997, Moscow, Russia.; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia., Roshchin MV; Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, 117485, Moscow, Russia., Smirnova GR; Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, 117485, Moscow, Russia., Kolotova DE; Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, 117485, Moscow, Russia.; Faculty of Biology, Moscow State University, 119234, Moscow, Russia., Balaban PM; Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, 117485, Moscow, Russia., Ostrovsky MA; Faculty of Biology, Moscow State University, 119234, Moscow, Russia.; Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, 119334, Moscow, Russia., Malyshev AY; Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, 117485, Moscow, Russia. malyshev@ihna.ru. |
| المصدر: | Doklady. Biochemistry and biophysics [Dokl Biochem Biophys] 2019 May; Vol. 486 (1), pp. 184-186. Date of Electronic Publication: 2019 Jul 31. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Pleiades Publishing Country of Publication: United States NLM ID: 101126895 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1608-3091 (Electronic) Linking ISSN: 16076729 NLM ISO Abbreviation: Dokl Biochem Biophys Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York, NY : Pleiades Publishing Original Publication: Moscow, Russia : [Dordrecht] : International Academic Pub. Co. "Nauka/Interperiodica" ; Distributed worldwide by Kluwer Academic/Plenum Publishers, c2001- |
| مواضيع طبية MeSH: | Channelrhodopsins/*genetics , Optogenetics/*methods , Retina/*pathology , Retinal Ganglion Cells/*metabolism, Animals ; Light ; Neurons/cytology ; Neurons/metabolism ; Neurons/radiation effects ; Rats ; Retina/radiation effects ; Retinal Ganglion Cells/pathology ; Retinal Ganglion Cells/radiation effects ; Transfection |
| مستخلص: | To perform optogenetic prosthetics of the retinal ganglion cell receptive field, a bicistronic genetic construct carrying the genes encoding the excitatory (channelrhodopsin-2) and inhibitory (Guillardia theta anion channelrhodopsin GtACR2) rhodopsins was created. A characteristic feature of this construct was the combination of these two genes with a mutant IRES insertion between them, which ensures the exact ratio of expression levels of the first and second genes in each transfected cell. Illumination of the central part of the neuron with light with a wavelength of 470 nm induced the action potential generation in the cell. Stimulation of the peripheral neuronal region with light induced the inhibition of action potential generation. Thus, using optogenetics methods, we simulated the ON-OFF interaction in the retinal ganglion cell receptive field. Theoretically, this construct can be used for optogenetic prosthetics of degenerative retina in the case of its delivery to the ganglion cells with lentiviral vectors. |
| References: | Neuron. 2011 Feb 24;69(4):713-20. (PMID: 21338881) PLoS One. 2013 Jun 14;8(6):e66332. (PMID: 23799092) PLoS One. 2013 Dec 09;8(12):e82100. (PMID: 24349195) Science. 2015 Aug 7;349(6248):647-50. (PMID: 26113638) Dokl Biochem Biophys. 2016 Nov;471(1):440-442. (PMID: 28058692) |
| المشرفين على المادة: | 0 (Channelrhodopsins) |
| تواريخ الأحداث: | Date Created: 20190802 Date Completed: 20190829 Latest Revision: 20200225 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1134/S1607672919030062 |
| PMID: | 31367817 |
| قاعدة البيانات: | MEDLINE |
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