دورية أكاديمية
Cdc42 defines apical identity and regulates epithelial morphogenesis by promoting apical recruitment of Par6-aPKC and Crumbs.
| العنوان: | Cdc42 defines apical identity and regulates epithelial morphogenesis by promoting apical recruitment of Par6-aPKC and Crumbs. |
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| المؤلفون: | Nunes de Almeida F; MRC - Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, UK., Walther RF; MRC - Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, UK r.walther@ucl.ac.uk f.pichaud@ucl.ac.uk., Pressé MT; MRC - Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, UK., Vlassaks E; MRC - Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, UK., Pichaud F; MRC - Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, UK r.walther@ucl.ac.uk f.pichaud@ucl.ac.uk.; Institute for the Physics of Living Systems, University College London, London WC1E 6BT, UK. |
| المصدر: | Development (Cambridge, England) [Development] 2019 Aug 12; Vol. 146 (15). Date of Electronic Publication: 2019 Aug 12. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Company Of Biologists Limited Country of Publication: England NLM ID: 8701744 Publication Model: Electronic Cited Medium: Internet ISSN: 1477-9129 (Electronic) Linking ISSN: 09501991 NLM ISO Abbreviation: Development Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge Eng : Company Of Biologists Limited Original Publication: [Cambridge] : Company of Biologists, [c1987- |
| مواضيع طبية MeSH: | Drosophila Proteins/*metabolism , Drosophila melanogaster/*growth & development , GTP-Binding Proteins/*metabolism , Membrane Proteins/*metabolism , Photoreceptor Cells/*cytology , Protein Kinase C/*metabolism, Animals ; Cell Polarity/physiology ; Drosophila melanogaster/metabolism ; Epithelium/growth & development ; Morphogenesis/physiology ; Protein Binding/physiology |
| مستخلص: | Cdc42 regulates epithelial morphogenesis together with the Par complex (Baz/Par3-Par6-aPKC), Crumbs (Crb/CRB3) and Stardust (Sdt/PALS1). However, how these proteins work together and interact during epithelial morphogenesis is not well understood. To address this issue, we used the genetically amenable Drosophila pupal photoreceptor and follicular epithelium. We show that during epithelial morphogenesis active Cdc42 accumulates at the developing apical membrane and cell-cell contacts, independently of the Par complex and Crb. However, membrane localization of Baz, Par6-aPKC and Crb all depend on Cdc42. We find that although binding of Cdc42 to Par6 is not essential for the recruitment of Par6 and aPKC to the membrane, it is required for their apical localization and accumulation, which we find also depends on Par6 retention by Crb. In the pupal photoreceptor, membrane recruitment of Par6-aPKC also depends on Baz. Our work shows that Cdc42 is required for this recruitment and suggests that this factor promotes the handover of Par6-aPKC from Baz onto Crb. Altogether, we propose that Cdc42 drives morphogenesis by conferring apical identity, Par-complex assembly and apical accumulation of Crb. Competing Interests: Competing interestsThe authors declare no competing or financial interests. (© 2019. Published by The Company of Biologists Ltd.) |
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| معلومات مُعتمدة: | MC_UU_00012/3 United Kingdom MRC_ Medical Research Council; MC_UU_12018/3 United Kingdom MRC_ Medical Research Council; P40 OD018537 United States OD NIH HHS; MC_UU_12018/3 United Kingdom MRC_ Medical Research Council |
| فهرسة مساهمة: | Keywords: Bazooka; Cdc42; Crumbs; Epithelial polarity; Par complex; Par3; Par6; aPKC |
| المشرفين على المادة: | 0 (Cdc42 protein, Drosophila) 0 (Drosophila Proteins) 0 (Membrane Proteins) 0 (crb protein, Drosophila) EC 2.7.11.13 (Par-6 protein, Drosophila) EC 2.7.11.13 (Protein Kinase C) EC 2.7.11.13 (aPKC protein, Drosophila) EC 3.6.1.- (GTP-Binding Proteins) |
| تواريخ الأحداث: | Date Created: 20190814 Date Completed: 20200623 Latest Revision: 20240419 |
| رمز التحديث: | 20240419 |
| مُعرف محوري في PubMed: | PMC6703713 |
| DOI: | 10.1242/dev.175497 |
| PMID: | 31405903 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1477-9129 |
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| DOI: | 10.1242/dev.175497 |