دورية أكاديمية
Distinct mechanisms of microRNA sorting into cancer cell-derived extracellular vesicle subtypes.
العنوان: | Distinct mechanisms of microRNA sorting into cancer cell-derived extracellular vesicle subtypes. |
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المؤلفون: | Temoche-Diaz MM; Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, United States., Shurtleff MJ; Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, United States.; Department of Molecular and Cellular Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, United States., Nottingham RM; Department of Molecular Biosciences, Institute for Cellular and Molecular Biology, University of Texas, Austin, United States., Yao J; Department of Molecular Biosciences, Institute for Cellular and Molecular Biology, University of Texas, Austin, United States., Fadadu RP; Department of Molecular and Cellular Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, United States., Lambowitz AM; Department of Molecular Biosciences, Institute for Cellular and Molecular Biology, University of Texas, Austin, United States., Schekman R; Department of Molecular and Cellular Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, United States. |
المصدر: | ELife [Elife] 2019 Aug 22; Vol. 8. Date of Electronic Publication: 2019 Aug 22. |
نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: eLife Sciences Publications, Ltd Country of Publication: England NLM ID: 101579614 Publication Model: Electronic Cited Medium: Internet ISSN: 2050-084X (Electronic) Linking ISSN: 2050084X NLM ISO Abbreviation: Elife Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Cambridge, UK : eLife Sciences Publications, Ltd., 2012- |
مواضيع طبية MeSH: | Extracellular Vesicles/*metabolism , MicroRNAs/*metabolism, Autoantigens/metabolism ; Biological Transport ; Breast Neoplasms/pathology ; Cell Line, Tumor ; Humans ; Protein Binding ; RNA-Binding Proteins/metabolism ; Ribonucleoproteins/metabolism ; SS-B Antigen |
مستخلص: | Extracellular vesicles (EVs) encompass a variety of vesicles secreted into the extracellular space. EVs have been implicated in promoting tumor metastasis, but the molecular composition of tumor-derived EV sub-types and the mechanisms by which molecules are sorted into EVs remain mostly unknown. We report the separation of two small EV sub-populations from a metastatic breast cancer cell line, with biochemical features consistent with different sub-cellular origins. These EV sub-types use different mechanisms of miRNA sorting (selective and non-selective), suggesting that sorting occurs via fundamentally distinct processes, possibly dependent on EV origin. Using biochemical and genetic tools, we identified the Lupus La protein as mediating sorting of selectively packaged miRNAs. We found that two motifs embedded in miR-122 are responsible for high-affinity binding to Lupus La and sorting into vesicles formed in a cell-free reaction. Thus, tumor cells can simultaneously deploy multiple EV species using distinct sorting mechanisms that may enable diverse functions in normal and cancer biology. Competing Interests: MT, MS, RN, JY, RF No competing interests declared, AL Thermostable group II intron reverse transcriptase (TGIRT) enzymes and methods for their use are the subject of patents and patent applications that have been licensed by the University of Texas and East Tennessee State University to InGex, LLC. AML, some former and present members of the AML laboratory, and the University of Texas are minority equity holders in InGex, LLC and receive royalty payments from the sale of TGIRT enzymes and kits and from the sublicensing of intellectual property to other companies. RS Reviewing Editor and Founding Editor-in-Chief, eLife (© 2019, Temoche-Diaz et al.) |
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معلومات مُعتمدة: | R01 GM037949 United States GM NIGMS NIH HHS; R01 GM37949 United States NH NIH HHS |
فهرسة مساهمة: | Keywords: Lupus La; biochemistry; cancer biology; chemical biology; extracellular vesicles; human; miRNA; multivesicular bodies |
سلسلة جزيئية: | SRA PRJNA532890 |
المشرفين على المادة: | 0 (Autoantigens) 0 (MicroRNAs) 0 (RNA-Binding Proteins) 0 (Ribonucleoproteins) |
تواريخ الأحداث: | Date Created: 20190823 Date Completed: 20200219 Latest Revision: 20231213 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC6728143 |
DOI: | 10.7554/eLife.47544 |
PMID: | 31436530 |
قاعدة البيانات: | MEDLINE |
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