دورية أكاديمية
Distinct mechanisms of microRNA sorting into cancer cell-derived extracellular vesicle subtypes.
| العنوان: | Distinct mechanisms of microRNA sorting into cancer cell-derived extracellular vesicle subtypes. |
|---|---|
| المؤلفون: | Temoche-Diaz MM; Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, United States., Shurtleff MJ; Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, United States.; Department of Molecular and Cellular Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, United States., Nottingham RM; Department of Molecular Biosciences, Institute for Cellular and Molecular Biology, University of Texas, Austin, United States., Yao J; Department of Molecular Biosciences, Institute for Cellular and Molecular Biology, University of Texas, Austin, United States., Fadadu RP; Department of Molecular and Cellular Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, United States., Lambowitz AM; Department of Molecular Biosciences, Institute for Cellular and Molecular Biology, University of Texas, Austin, United States., Schekman R; Department of Molecular and Cellular Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, United States. |
| المصدر: | ELife [Elife] 2019 Aug 22; Vol. 8. Date of Electronic Publication: 2019 Aug 22. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: eLife Sciences Publications, Ltd Country of Publication: England NLM ID: 101579614 Publication Model: Electronic Cited Medium: Internet ISSN: 2050-084X (Electronic) Linking ISSN: 2050084X NLM ISO Abbreviation: Elife Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge, UK : eLife Sciences Publications, Ltd., 2012- |
| مواضيع طبية MeSH: | Extracellular Vesicles/*metabolism , MicroRNAs/*metabolism, Autoantigens/metabolism ; Biological Transport ; Breast Neoplasms/pathology ; Cell Line, Tumor ; Humans ; Protein Binding ; RNA-Binding Proteins/metabolism ; Ribonucleoproteins/metabolism ; SS-B Antigen |
| مستخلص: | Extracellular vesicles (EVs) encompass a variety of vesicles secreted into the extracellular space. EVs have been implicated in promoting tumor metastasis, but the molecular composition of tumor-derived EV sub-types and the mechanisms by which molecules are sorted into EVs remain mostly unknown. We report the separation of two small EV sub-populations from a metastatic breast cancer cell line, with biochemical features consistent with different sub-cellular origins. These EV sub-types use different mechanisms of miRNA sorting (selective and non-selective), suggesting that sorting occurs via fundamentally distinct processes, possibly dependent on EV origin. Using biochemical and genetic tools, we identified the Lupus La protein as mediating sorting of selectively packaged miRNAs. We found that two motifs embedded in miR-122 are responsible for high-affinity binding to Lupus La and sorting into vesicles formed in a cell-free reaction. Thus, tumor cells can simultaneously deploy multiple EV species using distinct sorting mechanisms that may enable diverse functions in normal and cancer biology. Competing Interests: MT, MS, RN, JY, RF No competing interests declared, AL Thermostable group II intron reverse transcriptase (TGIRT) enzymes and methods for their use are the subject of patents and patent applications that have been licensed by the University of Texas and East Tennessee State University to InGex, LLC. AML, some former and present members of the AML laboratory, and the University of Texas are minority equity holders in InGex, LLC and receive royalty payments from the sale of TGIRT enzymes and kits and from the sublicensing of intellectual property to other companies. RS Reviewing Editor and Founding Editor-in-Chief, eLife (© 2019, Temoche-Diaz et al.) |
| References: | Cold Spring Harb Protoc. 2014 Apr 01;2014(4):435-40. (PMID: 24692495) Elife. 2015 Jul 01;4:e07197. (PMID: 26132860) J Cell Biol. 1985 Sep;101(3):942-8. (PMID: 2993317) Cell. 2013 Jul 18;154(2):442-51. (PMID: 23849981) RNA. 2013 Jul;19(7):958-70. (PMID: 23697550) Cell. 2004 Jan 23;116(2):281-97. (PMID: 14744438) J Extracell Vesicles. 2014 Sep 18;3:. (PMID: 25317274) Traffic. 2006 Nov;7(11):1440-50. (PMID: 16984406) Blood. 2012 Jan 19;119(3):756-66. (PMID: 22031862) Cell Rep. 2016 Oct 11;17(3):799-808. (PMID: 27732855) Nature. 2012 May 02;485(7396):109-13. (PMID: 22552098) J Biol Chem. 2003 Sep 12;278(37):35145-51. (PMID: 12840030) J Proteomics. 2013 Mar 27;80:171-82. (PMID: 23333927) Nat Cell Biol. 2010 Jan;12(1):19-30; sup pp 1-13. (PMID: 19966785) Oncogene. 2011 Jan 27;30(4):434-44. (PMID: 20856207) Nat Cell Biol. 2009 Sep;11(9):1143-9. (PMID: 19684575) Mol Cell. 2003 Nov;12(5):1301-7. (PMID: 14636586) Trends Cell Biol. 2009 Feb;19(2):43-51. (PMID: 19144520) Cancer Res. 2014 Oct 15;74(20):5758-71. (PMID: 25261234) EMBO Rep. 2016 Aug;17(8):1184-203. (PMID: 27402548) Nucleic Acids Res. 2012 Oct;40(19):9738-49. (PMID: 22904067) Cancer Cell. 2003 Feb;3(2):145-60. (PMID: 12620409) Oncogene. 2017 Aug 24;36(34):4929-4942. (PMID: 28436951) Proc Natl Acad Sci U S A. 2016 Feb 2;113(5):E587-96. (PMID: 26768848) Mol Biol Cell. 2017 Oct 1;28(20):2723-2733. (PMID: 28768830) PLoS One. 2011;6(10):e25402. (PMID: 22016766) J Cell Biol. 2017 Jun 5;216(6):1745-1759. (PMID: 28428367) RNA. 2016 Jan;22(1):111-28. (PMID: 26554030) Bioinformatics. 2004 Dec 12;20(18):3710-5. (PMID: 15297299) Nat Biotechnol. 2013 Sep;31(9):827-32. (PMID: 23873081) Nucleic Acids Res. 2012 Jan;40(1):290-302. (PMID: 21896617) Int Immunol. 2005 Jul;17(7):879-87. (PMID: 15908444) Nat Rev Microbiol. 2005 Aug;3(8):643-55. (PMID: 16064056) Cell. 1984 Jan;36(1):145-54. (PMID: 6607117) J Cell Biol. 2010 Apr 19;189(2):223-32. (PMID: 20404108) Cell. 2019 Apr 4;177(2):428-445.e18. (PMID: 30951670) Cell Rep. 2014 Sep 11;8(5):1432-46. (PMID: 25159140) Proc Int Conf Intell Syst Mol Biol. 1994;2:28-36. (PMID: 7584402) Nat Commun. 2013;4:2980. (PMID: 24356509) Sci Rep. 2016 Mar 02;6:22519. (PMID: 26931825) Cancer Cell. 2014 Apr 14;25(4):501-15. (PMID: 24735924) J Cell Biol. 2013 Feb 18;200(4):373-83. (PMID: 23420871) Nucleic Acids Res. 2015 Jun 23;43(11):5601-16. (PMID: 25940616) Int J Mol Sci. 2015 Feb 05;16(2):3537-63. (PMID: 25664860) J Extracell Vesicles. 2012 Apr 16;1:. (PMID: 24009879) Mol Cell Proteomics. 2013 Feb;12(2):343-55. (PMID: 23161513) J Cell Biol. 1983 Aug;97(2):329-39. (PMID: 6309857) Elife. 2016 Aug 25;5:. (PMID: 27559612) Clin Cancer Res. 2007 Sep 15;13(18 Pt 2):5509s-5518s. (PMID: 17875783) PLoS One. 2012;7(10):e47053. (PMID: 23056576) PLoS One. 2011;6(7):e21800. (PMID: 21789182) J Immunol. 2007 Aug 1;179(3):1969-78. (PMID: 17641064) Neoplasia. 2013 Mar;15(3):281-95. (PMID: 23479506) Proc Natl Acad Sci U S A. 2010 Apr 6;107(14):6328-33. (PMID: 20304794) Elife. 2016 Sep 23;5:. (PMID: 27661255) Nat Methods. 2012 Mar 04;9(4):357-9. (PMID: 22388286) Genes Chromosomes Cancer. 2014 Sep;53(9):713-24. (PMID: 24764226) Nucleic Acids Res. 2000 Aug 1;28(15):2927-34. (PMID: 10908356) Sci Rep. 2016 Aug 09;6:31175. (PMID: 27503761) Cell Rep. 2014 Sep 25;8(6):1649-1658. (PMID: 25242326) Proc Natl Acad Sci U S A. 2016 Feb 23;113(8):E968-77. (PMID: 26858453) Nucleic Acids Res. 2014 Apr;42(7):4629-39. (PMID: 24464996) Annu Rev Biochem. 2002;71:375-403. (PMID: 12045101) Nat Cell Biol. 2015 Feb;17(2):183-94. (PMID: 25621950) J Pathol. 2007 Apr;211(5):582-90. (PMID: 17334982) Haematologica. 2011 Sep;96(9):1302-9. (PMID: 21606166) Oncotarget. 2017 Feb 28;8(9):15252-15266. (PMID: 28146434) Nat Methods. 2015 Apr;12(4):357-60. (PMID: 25751142) Nat Protoc. 2006;1(2):577-80. (PMID: 17406284) Oncogene. 2009 Oct 8;28(40):3526-36. (PMID: 19617899) Proc Natl Acad Sci U S A. 2007 Jul 31;104(31):12913-8. (PMID: 17646655) Nat Commun. 2015 Apr 01;6:6716. (PMID: 25828099) Cell. 2014 Oct 23;159(3):647-61. (PMID: 25307932) Proc Natl Acad Sci U S A. 2017 Oct 24;114(43):E8987-E8995. (PMID: 29073095) Bioinformatics. 2009 Aug 15;25(16):2078-9. (PMID: 19505943) Annu Rev Cell Dev Biol. 2014;30:255-89. (PMID: 25288114) Bioinformatics. 2010 Mar 15;26(6):841-2. (PMID: 20110278) Nucleic Acids Res. 1985 Apr 11;13(7):2617-29. (PMID: 2582364) Nat Rev Cancer. 2017 May;17(5):302-317. (PMID: 28303905) Nucleic Acids Res. 2009 Dec;37(22):7533-45. (PMID: 19808937) J Transl Med. 2012 Mar 08;10:42. (PMID: 22400902) Nat Commun. 2011;2:282. (PMID: 21505438) Proc Natl Acad Sci U S A. 2004 Sep 7;101(36):13368-73. (PMID: 15326289) Proc Natl Acad Sci U S A. 2013 Jul 30;110(31):E2925-33. (PMID: 23818640) |
| معلومات مُعتمدة: | R01 GM037949 United States GM NIGMS NIH HHS; R01 GM37949 United States NH NIH HHS |
| فهرسة مساهمة: | Keywords: Lupus La; biochemistry; cancer biology; chemical biology; extracellular vesicles; human; miRNA; multivesicular bodies |
| سلسلة جزيئية: | SRA PRJNA532890 |
| المشرفين على المادة: | 0 (Autoantigens) 0 (MicroRNAs) 0 (RNA-Binding Proteins) 0 (Ribonucleoproteins) |
| تواريخ الأحداث: | Date Created: 20190823 Date Completed: 20200219 Latest Revision: 20231213 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC6728143 |
| DOI: | 10.7554/eLife.47544 |
| PMID: | 31436530 |
| قاعدة البيانات: | MEDLINE |
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