دورية أكاديمية
The pathogenicity of two Neospora caninum goat strains in a BALB/c mouse model.
| العنوان: | The pathogenicity of two Neospora caninum goat strains in a BALB/c mouse model. |
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| المؤلفون: | Costa RC; Department of Pathology- School of Veterinary Medicine and and Animal Sciences, FMVZ-USP, São Paulo, SP, Brazil. Electronic address: rafaelccosta3@gmail.com., Mesquita LP; Department of Pathology- School of Veterinary Medicine and and Animal Sciences, FMVZ-USP, São Paulo, SP, Brazil. Electronic address: leopmesquita@gmail.com., de Oliveira IM Jr; Department of Veterinary Medicine- Federal University of Lavras- UFLA, Lavras, MG, Brazil. Electronic address: moreira.ivam@gmail.com., Zannato DA; Department of Pathology- School of Veterinary Medicine and and Animal Sciences, FMVZ-USP, São Paulo, SP, Brazil. Electronic address: dennis.zanatto@usp.br., Emanuel Dos Santos Mesquita L; Department of Veterinary Medicine- Federal University of Lavras- UFLA, Lavras, MG, Brazil. Electronic address: mesquitalucas97@gmail.com., Biihrer DA; Department of Pathology- School of Veterinary Medicine and and Animal Sciences, FMVZ-USP, São Paulo, SP, Brazil. Electronic address: arrais.daniel@gmail.com., de Oliveira Massoco Salles Gomes C; Department of Pathology- School of Veterinary Medicine and and Animal Sciences, FMVZ-USP, São Paulo, SP, Brazil. Electronic address: cmassoco@gmail.com., Varaschin MS; Department of Veterinary Medicine- Federal University of Lavras- UFLA, Lavras, MG, Brazil. Electronic address: msvaraschin@dmv.ufla.br., Maiorka PC; Department of Pathology- School of Veterinary Medicine and and Animal Sciences, FMVZ-USP, São Paulo, SP, Brazil. Electronic address: maiorka@usp.br. |
| المصدر: | Experimental parasitology [Exp Parasitol] 2019 Oct; Vol. 205, pp. 107736. Date of Electronic Publication: 2019 Aug 20. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0370713 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2449 (Electronic) Linking ISSN: 00144894 NLM ISO Abbreviation: Exp Parasitol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Orlando, FL : Academic Press Original Publication: New York. |
| مواضيع طبية MeSH: | Coccidiosis/*veterinary , Goat Diseases/*parasitology , Neospora/*pathogenicity, Animals ; Biological Assay/veterinary ; Brain/parasitology ; Brain/pathology ; Chlorocebus aethiops ; Coccidiosis/parasitology ; Coccidiosis/pathology ; Disease Models, Animal ; Female ; Gene Expression Regulation ; Gerbillinae ; Goat Diseases/pathology ; Goats ; Immunohistochemistry/veterinary ; Interferon-gamma/genetics ; Interferon-gamma/metabolism ; Liver/pathology ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Mice, Knockout ; Neospora/isolation & purification ; Pancreas/pathology ; Placenta/pathology ; Pregnancy ; Spleen/metabolism ; Spleen/pathology ; Tumor Necrosis Factor-alpha/genetics ; Tumor Necrosis Factor-alpha/metabolism ; Vero Cells |
| مستخلص: | Goats are frequently described as an intermediate host for the protozoan Neospora caninum, manifesting the disease mainly by recurrent abortions with placentitis and encephalitis in fetuses. Several reports of natural and experimental infections in cattle and mice show differences in the immune response, and the outcome of the infection can be variable depending on the species affected and by the behavior of the infective strain. This study describes for the first time two Neospora caninum strains isolated from naturally infected goats from the state of Minas Gerais, Brazil. One placenta and one brain from different goats were processed for a first bioassay in gerbils (Meriones unguiculatus). Subsequently, a second bioassay was performed by inoculating the processed brain samples from gerbils into Interferon gamma (IFN-γ) knockout mice (KO mice). Tachyzoites collected from the peritoneal fluid of the KO mice were inoculated into VERO cell monolayers, where they presented a very slow growth rate. The tachyzoites were also inoculated into BALB/c mice with a dose of 10 6 tachyzoites per animal. After a 5-week follow up, the animals infected with both of the strains developed a strong polarized Th1 response with increased serum and spleen gene expression levels of pro-inflammatory cytokines (mainly IFN-γ and TNF-α) in the first week. Tissue lesions were mild in the animals infected with both strains. Despite the strong immune response preventing an infection in the visceral organs, the parasite was able to reach the brain, causing progressive brain lesions from the second to fifth week post infection. The NC-goat1-infected mice presented with severe meningoencephalitis, but the NC-goat2-infected animals had considerable histological brain lesions only at week 5. Immunohistochemical analysis of the mouse brains revealed a different pattern of inflammatory cells compared to the naturally infected goats. A severe inflammatory infiltrate of CD3 + T lymphocytes was found in the NC-goat1-infected mice. A more discrete infiltrate of CD3 + T cells was found in the NC-goat2-infected animals. Additionally, IBA1 IHC revealed an intense microglial reaction and monocyte perivascular cuffs in the NC-goat1-infected animals and lower microglia/monocyte infiltrates in the NC-goat2-infected mice. This work contributes knowledge on the pathogenicity of new Neospora caninum strains in mice, comparable with other well-established mouse models of the disease, and demonstrates the importance of studying goats as an intermediate host of this parasite. (Copyright © 2019 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Apicomplexa; Capra hircus; Encephalitis; Flow cytometry; Gene expression; Immunohistochemistry |
| المشرفين على المادة: | 0 (Tumor Necrosis Factor-alpha) 82115-62-6 (Interferon-gamma) |
| تواريخ الأحداث: | Date Created: 20190824 Date Completed: 20191003 Latest Revision: 20191210 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.exppara.2019.107736 |
| PMID: | 31442455 |
| قاعدة البيانات: | MEDLINE |
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