دورية أكاديمية
Lysosome-Rich Enterocytes Mediate Protein Absorption in the Vertebrate Gut.
| العنوان: | Lysosome-Rich Enterocytes Mediate Protein Absorption in the Vertebrate Gut. |
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| المؤلفون: | Park J; Department of Cell Biology, Duke University, Durham, NC 27710, USA; Department of Pharmacology and Cancer Biology, Duke University, Durham, NC 27710, USA., Levic DS; Department of Cell Biology, Duke University, Durham, NC 27710, USA., Sumigray KD; Department of Dermatology, Duke University Medical Center, Durham, NC 27710, USA., Bagwell J; Department of Cell Biology, Duke University, Durham, NC 27710, USA., Eroglu O; Department of Cell Biology, Duke University, Durham, NC 27710, USA., Block CL; Department of Psychology and Neuroscience, Duke University, Durham, NC 27710, USA., Eroglu C; Department of Cell Biology, Duke University, Durham, NC 27710, USA; Department of Neurobiology, Duke University, Durham, NC 27710, United States; Regeneration Next Initiative, Duke University, Durham, NC 27710, United States., Barry R; Department of Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA., Lickwar CR; Department of Molecular Genetics and Microbiology, Duke University, Durham, NC 27710, USA., Rawls JF; Department of Molecular Genetics and Microbiology, Duke University, Durham, NC 27710, USA., Watts SA; Department of Biology, University of Alabama at Birmingham, Birmingham, AL 35294, USA., Lechler T; Department of Cell Biology, Duke University, Durham, NC 27710, USA; Department of Dermatology, Duke University Medical Center, Durham, NC 27710, USA., Bagnat M; Department of Cell Biology, Duke University, Durham, NC 27710, USA; Regeneration Next Initiative, Duke University, Durham, NC 27710, United States. Electronic address: michel.bagnat@duke.edu. |
| المصدر: | Developmental cell [Dev Cell] 2019 Oct 07; Vol. 51 (1), pp. 7-20.e6. Date of Electronic Publication: 2019 Aug 29. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101120028 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-1551 (Electronic) Linking ISSN: 15345807 NLM ISO Abbreviation: Dev Cell Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge, Mass. : Cell Press, c2001- |
| مواضيع طبية MeSH: | Intestinal Absorption*, Dietary Proteins/*metabolism , Enterocytes/*metabolism , Intestines/*embryology , Lysosomes/*metabolism, Adaptor Proteins, Signal Transducing/metabolism ; Adaptor Proteins, Vesicular Transport/metabolism ; Animals ; Apoptosis Regulatory Proteins/metabolism ; Disease Models, Animal ; Female ; Gastrointestinal Microbiome ; Gene Deletion ; Gene Expression Regulation, Developmental ; Ileum/embryology ; Ileum/metabolism ; Kwashiorkor/metabolism ; Ligands ; Male ; Membrane Proteins/metabolism ; Mice ; Receptors, Cell Surface/metabolism ; Zebrafish ; Zebrafish Proteins/metabolism |
| مستخلص: | The guts of neonatal mammals and stomachless fish have a limited capacity for luminal protein digestion, which allows oral acquisition of antibodies and antigens. However, how dietary protein is absorbed during critical developmental stages when the gut is still immature is unknown. Here, we show that specialized intestinal cells, which we call lysosome-rich enterocytes (LREs), internalize dietary protein via receptor-mediated and fluid-phase endocytosis for intracellular digestion and trans-cellular transport. In LREs, we identify a conserved endocytic machinery, composed of the scavenger receptor complex Cubilin/Amnionless and Dab2, that is required for protein uptake by LREs and for growth and survival of larval zebrafish. Moreover, impairing LRE function in suckling mice, via conditional deletion of Dab2, leads to stunted growth and severe protein malnutrition reminiscent of kwashiorkor, a devastating human malnutrition syndrome. These findings identify digestive functions and conserved molecular mechanisms in LREs that are crucial for vertebrate growth and survival. (Copyright © 2019 Elsevier Inc. All rights reserved.) |
| التعليقات: | Comment in: Dev Cell. 2019 Oct 7;51(1):1-2. (PMID: 31593648) |
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| معلومات مُعتمدة: | P01 DK094779 United States DK NIDDK NIH HHS; F31 DK111137 United States DK NIDDK NIH HHS; United States HHMI Howard Hughes Medical Institute; R01 DK081426 United States DK NIDDK NIH HHS; R01 DK117981 United States DK NIDDK NIH HHS; R01 DK113123 United States DK NIDDK NIH HHS; R01 AR055926 United States AR NIAMS NIH HHS; T32 DK007568 United States DK NIDDK NIH HHS; R01 AR067203 United States AR NIAMS NIH HHS; R01 DK121007 United States DK NIDDK NIH HHS |
| فهرسة مساهمة: | Keywords: Cubilin; Dab2; inter-organ transport; intestine; kwashiorkor; lysosome-rich enterocytes (LREs); malnutrition; mouse; protein absorption; zebrafish |
| المشرفين على المادة: | 0 (Adaptor Proteins, Signal Transducing) 0 (Adaptor Proteins, Vesicular Transport) 0 (Amn protein, mouse) 0 (Apoptosis Regulatory Proteins) 0 (Dab2 protein, mouse) 0 (Dab2 protein, zebrafish) 0 (Dietary Proteins) 0 (Ligands) 0 (Membrane Proteins) 0 (Receptors, Cell Surface) 0 (Zebrafish Proteins) 0 (intrinsic factor-cobalamin receptor) |
| تواريخ الأحداث: | Date Created: 20190903 Date Completed: 20200527 Latest Revision: 20230413 |
| رمز التحديث: | 20230413 |
| مُعرف محوري في PubMed: | PMC6783362 |
| DOI: | 10.1016/j.devcel.2019.08.001 |
| PMID: | 31474562 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1878-1551 |
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| DOI: | 10.1016/j.devcel.2019.08.001 |