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Identification and relative abundances of mRNA for a gene encoding the vWD domain and three Kazal-type domains in the ovary of giant freshwater prawns, Macrobrachium rosenbergii.

التفاصيل البيبلوغرافية
العنوان: Identification and relative abundances of mRNA for a gene encoding the vWD domain and three Kazal-type domains in the ovary of giant freshwater prawns, Macrobrachium rosenbergii.
المؤلفون: Faiz ZM; Institute of Tropical Aquaculture, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, Terengganu, Malaysia. Electronic address: faizzakaria92@gmail.com., Mardhiyyah MP; Institute of Tropical Aquaculture, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, Terengganu, Malaysia. Electronic address: mpmardhiyyah@gmail.com., Mohamad A; Institute of Bioscience, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia. Electronic address: aslahumt@gmail.com., Hidir A; Institute of Tropical Aquaculture, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, Terengganu, Malaysia. Electronic address: hidirariffin@yahoo.com., Nurul-Hidayah A; Institute of Tropical Aquaculture, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, Terengganu, Malaysia. Electronic address: nurulhidayahamirdin@gmail.com., Wong L; Institute of Tropical Aquaculture, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, Terengganu, Malaysia. Electronic address: lilian@umt.edu.my., Jasmani S; Institute of Tropical Aquaculture, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, Terengganu, Malaysia. Electronic address: safia@umt.edu.my., Ikhwanuddin M; Institute of Tropical Aquaculture, Universiti Malaysia Terengganu, 21030, Kuala Nerus, Terengganu, Terengganu, Malaysia. Electronic address: ikhwanuddin@umt.edu.my.
المصدر: Animal reproduction science [Anim Reprod Sci] 2019 Oct; Vol. 209, pp. 106143. Date of Electronic Publication: 2019 Aug 01.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Scientific Pub. Co Country of Publication: Netherlands NLM ID: 7807205 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2232 (Electronic) Linking ISSN: 03784320 NLM ISO Abbreviation: Anim Reprod Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam, Elsevier Scientific Pub. Co.
مواضيع طبية MeSH: Kazal Motifs/*genetics , Ovary/*metabolism , Palaemonidae/*genetics , Sex Differentiation/*genetics , von Willebrand Factor/*chemistry, Amino Acid Sequence ; Animals ; Base Sequence ; Cloning, Molecular ; Female ; Fresh Water ; Gene Expression Regulation, Developmental ; Hepatopancreas/embryology ; Hepatopancreas/growth & development ; Hepatopancreas/metabolism ; Ovary/embryology ; Ovary/growth & development ; Palaemonidae/embryology ; Palaemonidae/growth & development ; Protein Domains/genetics ; RNA, Messenger/genetics ; Sexual Maturation/genetics ; von Willebrand Factor/genetics
مستخلص: Understanding Macrobrachium rosenbergii ovarian maturation control at the genome level is an important aspect for increasing larvae production. In this study, an ovarian maturation related gene, M. rosenbergii vWD domain and three Kazal-type domains of a gene (MrvWD-Kazal) have been studied. The MrvWD-Kazal gene was isolated using a rapid amplification of cDNA end (RACE) method and the relative abundances of MrvWD-Kazal mRNA in the ovary, hepatopancreas, stomach, intestine and gill were determined by using the quantitative PCR technique. The MrvWD-Kazal gene is composed of 2194 bp with an open reading frame (ORF) of 1998 bp encoding 665 amino acids and has great similarity to the M. nipponense vWD-Kazal gene (91%). The qPCR analyses indicated the relative abundance of MrvWD-Kazal mRNA transcript varied among different stages of ovarian function (P < 0.05), but there were no differences abundance in hepatopancreas, stomach, intestine and gill (P> 0.05). In the ovary, relative abundance of MrvWD-Kazal mRNA transcript gradually increased with ovarian maturation from Stages 1 (Spent; 1.00-fold), to 2 (Proliferative; 3.47-fold) to 3 (Premature; 6.18-fold) and decreased at Stage 4 (Mature; 1.31-fold). Differential relative abundances of MrvWD-Kazal mRNA transcript in the ovary indicate the MrvWD-Kazal protein may have an important function in ovarian maturation of M. rosenbergii. The results of this study also indicate the MrvWD-Kazal is not involved in regulation of the reproductive related function of the hepatopancreas, digestive system (stomach and intestine) and respiratory system (gill).
(Copyright © 2019 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Kazal-type; Macrobrachium rosenbergii; Ovarian maturation; vWD
المشرفين على المادة: 0 (RNA, Messenger)
0 (von Willebrand Factor)
تواريخ الأحداث: Date Created: 20190914 Date Completed: 20200316 Latest Revision: 20200316
رمز التحديث: 20240628
DOI: 10.1016/j.anireprosci.2019.106143
PMID: 31514941
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-2232
DOI:10.1016/j.anireprosci.2019.106143