دورية أكاديمية
Comparison of Four Parasitological Techniques for Laboratory Diagnosis of Eggs from Spirometra spp. in Wild Mammal Fecal Samples.
| العنوان: | Comparison of Four Parasitological Techniques for Laboratory Diagnosis of Eggs from Spirometra spp. in Wild Mammal Fecal Samples. |
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| المؤلفون: | Dib LV; Department of Microbiology and Parasitology, Biomedical Institute, Fluminense Federal University, Rua Professor Hernani Mello, 101, São Domingos, Niterói, RJ, 24210-130, Brazil. laisvdib@gmail.com., Palmer JPS; Department of Microbiology and Parasitology, Biomedical Institute, Fluminense Federal University, Rua Professor Hernani Mello, 101, São Domingos, Niterói, RJ, 24210-130, Brazil., de Lima CSCC; Department of Microbiology and Parasitology, Biomedical Institute, Fluminense Federal University, Rua Professor Hernani Mello, 101, São Domingos, Niterói, RJ, 24210-130, Brazil., Ramos RCF; Department of Microbiology and Parasitology, Biomedical Institute, Fluminense Federal University, Rua Professor Hernani Mello, 101, São Domingos, Niterói, RJ, 24210-130, Brazil.; Laboratory for Toxoplasmosis and Other Protozoan Diseases, Oswaldo Cruz Institute, Oswaldo Cruz Foundation (Fiocruz Rio de Janeiro), Avenida Brasil 4365, Manguinhos, RJ, 21045-900, Brazil.; Statistics Laboratory, Mathematics and Statistics Institute, Fluminense Federal University, Rua Professor Marcos Waldemar de Freitas Reis s/n, bloco G, Gragoatá Campus, Niterói, RJ, 24210-201, Brazil., Bastos OMP; Department of Microbiology and Parasitology, Biomedical Institute, Fluminense Federal University, Rua Professor Hernani Mello, 101, São Domingos, Niterói, RJ, 24210-130, Brazil., Uchôa CMA; Department of Microbiology and Parasitology, Biomedical Institute, Fluminense Federal University, Rua Professor Hernani Mello, 101, São Domingos, Niterói, RJ, 24210-130, Brazil., Amendoeira MRR; Laboratory for Toxoplasmosis and Other Protozoan Diseases, Oswaldo Cruz Institute, Oswaldo Cruz Foundation (Fiocruz Rio de Janeiro), Avenida Brasil 4365, Manguinhos, RJ, 21045-900, Brazil., Fonseca ABM; Statistics Laboratory, Mathematics and Statistics Institute, Fluminense Federal University, Rua Professor Marcos Waldemar de Freitas Reis s/n, bloco G, Gragoatá Campus, Niterói, RJ, 24210-201, Brazil., Bastos ACMP; Department of Microbiology and Parasitology, Biomedical Institute, Fluminense Federal University, Rua Professor Hernani Mello, 101, São Domingos, Niterói, RJ, 24210-130, Brazil., Barbosa ADS; Department of Microbiology and Parasitology, Biomedical Institute, Fluminense Federal University, Rua Professor Hernani Mello, 101, São Domingos, Niterói, RJ, 24210-130, Brazil.; Laboratory for Toxoplasmosis and Other Protozoan Diseases, Oswaldo Cruz Institute, Oswaldo Cruz Foundation (Fiocruz Rio de Janeiro), Avenida Brasil 4365, Manguinhos, RJ, 21045-900, Brazil. |
| المصدر: | Acta parasitologica [Acta Parasitol] 2019 Dec; Vol. 64 (4), pp. 942-949. Date of Electronic Publication: 2019 Sep 13. |
| نوع المنشور: | Comparative Study; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer International Publishing Country of Publication: Switzerland NLM ID: 9301947 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1896-1851 (Electronic) Linking ISSN: 12302821 NLM ISO Abbreviation: Acta Parasitol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2019- : Cham : Springer International Publishing Original Publication: Warszawa : Witold Stefanski Institute Of Parasitology |
| مواضيع طبية MeSH: | Animals, Wild/*parasitology , Feces/*parasitology , Mammals/*parasitology , Parasite Egg Count/*methods , Spirometra/*isolation & purification, Animals ; Brazil ; Microscopy ; Parks, Recreational |
| مستخلص: | Purpose: This study aimed to compare the performance of four different microscopic coproparasitological techniques in relation to egg recovering and the frequency of alterations in the eggs observed through each technique. Methods: A total of 213 fecal samples from free-living carnivorous mammals were collected between 2017 and 2018 in Itatiaia National Park, RJ, Brazil. Faust and modified Sheather floatation techniques as well as Lutz and modified Ritchie sedimentation techniques were applied. Results: The total positivity rate for Spirometra spp. eggs was 24.4%, and these were detected mainly through Lutz and modified Ritchie sedimentation techniques, with substantial agreement (kappa = 0.73; p = 0.00 < 0.05; McNemar p value = 1.0; Fisher's exact test p = 0.616). Faust and modified Sheather flotation techniques did not present good egg recovery, with frequencies of 6.6% and 7.5%, respectively. Eggs with morphological alterations were mostly observed through Faust (17.3%) and modified Sheather (13.5%). Both flotation techniques presented statistically significant frequencies of deformed eggs, in comparison with the sedimentation techniques (p = 0.00). Low frequencies of deformed eggs were observed when the samples were analyzed through modified Ritchie and Lutz sedimentation techniques. Conclusions: From these results, sedimentation techniques such as modified Ritchie and Lutz methods were more efficient for diagnosing the eggs of this helminth in fecal material from free-living carnivores and should always be used when analyzing fecal samples from hosts of different species. |
| فهرسة مساهمة: | Keywords: Carnivores; Feces; Laboratory diagnosis; Spirometra; Wildlife |
| تواريخ الأحداث: | Date Created: 20190915 Date Completed: 20200507 Latest Revision: 20200507 |
| رمز التحديث: | 20221213 |
| DOI: | 10.2478/s11686-019-00120-1 |
| PMID: | 31520294 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1896-1851 |
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| DOI: | 10.2478/s11686-019-00120-1 |