دورية أكاديمية
Effects of nanofibrillated cellulose hydrogels on adipose tissue extract and hepatocellular carcinoma cell spheroids in freeze-drying.
| العنوان: | Effects of nanofibrillated cellulose hydrogels on adipose tissue extract and hepatocellular carcinoma cell spheroids in freeze-drying. |
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| المؤلفون: | Auvinen VV; Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, P.O. Box 56, 00014, Helsinki, Finland; Faculty of Engineering and Natural Sciences, Tampere University, P.O. Box 541, 33014, Tampereen yliopisto, Finland., Merivaara A; Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, P.O. Box 56, 00014, Helsinki, Finland., Kiiskinen J; Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, P.O. Box 56, 00014, Helsinki, Finland., Paukkonen H; Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, P.O. Box 56, 00014, Helsinki, Finland., Laurén P; Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, P.O. Box 56, 00014, Helsinki, Finland., Hakkarainen T; Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, P.O. Box 56, 00014, Helsinki, Finland., Koivuniemi R; Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, P.O. Box 56, 00014, Helsinki, Finland., Sarkanen R; Faculty of Medicine and Health Technology, Tampere University, Kauppi Campus, Arvo Ylpön katu 34, 33520, Tampere, Finland., Ylikomi T; Faculty of Medicine and Health Technology, Tampere University, Kauppi Campus, Arvo Ylpön katu 34, 33520, Tampere, Finland., Laaksonen T; Faculty of Engineering and Natural Sciences, Tampere University, P.O. Box 541, 33014, Tampereen yliopisto, Finland., Yliperttula M; Division of Pharmaceutical Biosciences, Faculty of Pharmacy, University of Helsinki, P.O. Box 56, 00014, Helsinki, Finland; Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Via Marzolo 5, 35131, Padova, Italy. Electronic address: marjo.yliperttula@helsinki.fi. |
| المصدر: | Cryobiology [Cryobiology] 2019 Dec; Vol. 91, pp. 137-145. Date of Electronic Publication: 2019 Sep 15. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0006252 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2392 (Electronic) Linking ISSN: 00112240 NLM ISO Abbreviation: Cryobiology Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2000- > : Amsterdam : Elsevier Original Publication: San Diego, CA : Academic Press |
| مواضيع طبية MeSH: | Adipose Tissue/*chemistry , Cellulose/*pharmacology , Cryopreservation/*methods , Hydrogels/*pharmacology , Spheroids, Cellular/*cytology , Tissue Extracts/*pharmacology, Carcinoma, Hepatocellular ; Cell Membrane/pathology ; Freeze Drying ; Hep G2 Cells ; Humans ; Hydrogels/chemistry ; Liver Neoplasms ; Nanofibers/chemistry ; Tumor Cells, Cultured ; Water/chemistry |
| مستخلص: | The aim of this study was to evaluate the effects of two nanofibrillated cellulose (NFC) hydrogels on two human derivatives during freeze-drying. Native NFC hydrogel is a suitable platform to culture 3D cell spheroids and a hydrogel processed further, called anionic NFC (ANFC) hydrogel, is an excellent platform for controlled release of proteins. Moreover, it has been shown to be compatible with freeze-drying when correct lyoprotectants are implemented. Freeze-drying is a method, where substance is first frozen, and then vacuum dried trough sublimation of water in order to achieve dry matter without the loss of the original three-dimensional structures. The first chosen human derivative was adipose tissue extract (ATE) which is a cell-free growth factor-rich preparation capable of promoting growth of regenerative cells. The release of growth factors from the freeze-dried mixture of ATE and ANFC was compared to that of non-freeze-dried control mixtures. The release profiles remained at the same level after freeze-drying. The second derivative was hepatocellular carcinoma (HepG2) cell spheroids which were evaluated before and after freeze-drying. The 3D structure of the HepG2 cell spheroids was preserved and the spheroids retained 18% of their metabolic activity after rehydration. However, the freeze-dried and rehydrated HepG2 cell spheroids did not proliferate and the cell membrane was damaged by fusion and formation of crystals. (Copyright © 2019. Published by Elsevier Inc.) |
| فهرسة مساهمة: | Keywords: 3D cell culture; Adipose tissue extract; Cell spheroids; Freeze-drying; Nanofibrillated cellulose |
| المشرفين على المادة: | 0 (Hydrogels) 0 (Tissue Extracts) 059QF0KO0R (Water) 9004-34-6 (Cellulose) |
| تواريخ الأحداث: | Date Created: 20190919 Date Completed: 20200414 Latest Revision: 20200414 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1016/j.cryobiol.2019.09.005 |
| PMID: | 31533026 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1090-2392 |
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| DOI: | 10.1016/j.cryobiol.2019.09.005 |