دورية أكاديمية
Alternative method in Galleria mellonella larvae to study biofilm infection and treatment.
| العنوان: | Alternative method in Galleria mellonella larvae to study biofilm infection and treatment. |
|---|---|
| المؤلفون: | Campos-Silva R; Laboratório de Biofilmes e Diversidade Microbiana, Faculdade de Farmácia and Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil., Brust FR; Laboratório de Biofilmes e Diversidade Microbiana, Faculdade de Farmácia and Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil., Trentin DS; Programa de Pós-Graduação em Biociências, Departamento de Ciências Básicas da Saúde, Universidade de Ciências da Saúde de Porto Alegre (UFCSPA), Porto Alegre, Brazil., Macedo AJ; Laboratório de Biofilmes e Diversidade Microbiana, Faculdade de Farmácia and Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil. Electronic address: alexandre.macedo@ufrgs.br. |
| المصدر: | Microbial pathogenesis [Microb Pathog] 2019 Dec; Vol. 137, pp. 103756. Date of Electronic Publication: 2019 Sep 20. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: England NLM ID: 8606191 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-1208 (Electronic) Linking ISSN: 08824010 NLM ISO Abbreviation: Microb Pathog Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London ; Orlando : Academic Press, c1986- |
| مواضيع طبية MeSH: | Biofilms/*growth & development , Larva/*microbiology , Moths/*microbiology , Staphylococcal Infections/*microbiology, Animals ; Colony Count, Microbial ; Disease Models, Animal ; Staphylococcus aureus/pathogenicity |
| مستخلص: | In vivo studies are crucial decision-maker step in order to translate in vitro data to an applied therapy. Considering this we describe a simple method that analyzes and quantifies biofilm formation inside the Galleria mellonella larvae. Toothbrush bristles were employed as an abiotic surface to mimic a medical device. A standardized inoculum of Staphylococcus aureus was systemically injected in the larvae together with the insertion of a bristle in the last proleg pair. After incubation adhered cells were detached from bristles and quantified by colony-forming units (CFU) counting using staphylococci-selective medium. About 3 × 10 6 CFU of S. aureus were recovered from bristles and scanning electron microscopy (SEM) images confirmed biofilm formation. Control group did not show adherent bacteria, as demonstrated by absence of CFU counting and SEM images, indicating that the insertion procedure is free of bacterial contamination. We present a feasible method to evaluate bacterial biofilm formation in vivo that in the near future can be used to evaluate antibiofilm compounds. (Copyright © 2019 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Experimental method in vivo; Galleria mellonella; Microbial biofilm |
| تواريخ الأحداث: | Date Created: 20190924 Date Completed: 20200330 Latest Revision: 20200330 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.micpath.2019.103756 |
| PMID: | 31546000 |
| قاعدة البيانات: | MEDLINE |
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