دورية أكاديمية
A novel 3'-tRNA Glu -derived fragment acts as a tumor suppressor in breast cancer by targeting nucleolin.
| العنوان: | A novel 3'-tRNA Glu -derived fragment acts as a tumor suppressor in breast cancer by targeting nucleolin. |
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| المؤلفون: | Falconi M; School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy., Giangrossi M; School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy., Zabaleta ME; School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy., Wang J; School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy., Gambini V; School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy., Tilio M; School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy., Bencardino D; School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy., Occhipinti S; Department of Molecular Biotechnology and Health Sciences, Center for Experimental Research and Medical Studies, University of Torino, Torino, Italy., Belletti B; Division of Molecular Oncology, Centro di Riferimento Oncologico di Aviano (CRO) Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), National Cancer Institute, Aviano, Italy., Laudadio E; Dipartimento Scienze e Ingegneria della Materia, dell'Ambiente ed Urbanistica, Università Politecnica delle Marche, Ancona, Italy., Galeazzi R; Dipartimento di Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, Ancona, Italy., Marchini C; School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy., Amici A; School of Biosciences and Veterinary Medicine, University of Camerino, Camerino, Italy. |
| المصدر: | FASEB journal : official publication of the Federation of American Societies for Experimental Biology [FASEB J] 2019 Dec; Vol. 33 (12), pp. 13228-13240. Date of Electronic Publication: 2019 Sep 27. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Federation of American Societies for Experimental Biology Country of Publication: United States NLM ID: 8804484 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1530-6860 (Electronic) Linking ISSN: 08926638 NLM ISO Abbreviation: FASEB J Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2020- : [Bethesda, Md.] : Hoboken, NJ : Federation of American Societies for Experimental Biology ; Wiley Original Publication: [Bethesda, Md.] : The Federation, [c1987- |
| مواضيع طبية MeSH: | Breast Neoplasms/*metabolism , Phosphoproteins/*metabolism , RNA, Transfer, Glu/*metabolism , RNA-Binding Proteins/*metabolism, Animals ; Blotting, Western ; Breast Neoplasms/genetics ; Electrophoretic Mobility Shift Assay ; Gene Expression Regulation, Neoplastic/genetics ; Gene Expression Regulation, Neoplastic/physiology ; Humans ; Mice ; Mice, Transgenic ; Phosphoproteins/genetics ; RNA, Transfer, Glu/genetics ; RNA-Binding Proteins/genetics ; Reverse Transcriptase Polymerase Chain Reaction ; Nucleolin |
| مستخلص: | tRNA-derived fragments (tRFs) have been defined as a novel class of small noncoding RNAs. tRFs have been reported to be deregulated in cancer, but their biologic function remains to be fully understood. We have identified a new tRF (named tRF3E), derived from mature tRNA Glu , that is specifically expressed in healthy mammary glands but not in breast cancer (BC). Consistently, tRF3E levels significantly decrease in the blood of patients with epidermal growth factor receptor 2 (HER2)-positive BC reflecting tumor status (control > early cancer > metastatic cancer). tRF3E down-regulation was recapitulated in Δ16HER2 transgenic mice, representing a BC preclinical model. Pulldown assays, used to search for proteins capable to selectively bind tRF3E, have shown that this tRF specifically interacts with nucleolin (NCL), an RNA-binding protein overexpressed in BC and able to repress the translation of p53 mRNA. The binding properties of NCL-tRF3E complex, predicted in silico and analyzed by EMSA assays, are congruent with a competitive displacement of p53 mRNA by tRF3E, leading to an increased p53 expression and consequently to a modulation of cancer cell growth. Here, we provide evidence that tRF3E plays an important role in the pathogenesis of BC displaying tumor-suppressor functions through a NCL-mediated mechanism.-Falconi, M., Giangrossi, M., Elexpuru Zabaleta, M., Wang, J., Gambini, V., Tilio, M., Bencardino, D., Occhipinti, S., Belletti, B., Laudadio, E., Galeazzi, R., Marchini, C., Amici, A. A novel 3'-tRNA Glu -derived fragment acts as a tumor suppressor in breast cancer by targeting nucleolin. |
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| فهرسة مساهمة: | Keywords: HER2; RNA-protein interaction; p53 protein; small noncoding RNAs |
| المشرفين على المادة: | 0 (Phosphoproteins) 0 (RNA, Transfer, Glu) 0 (RNA-Binding Proteins) |
| تواريخ الأحداث: | Date Created: 20190928 Date Completed: 20200608 Latest Revision: 20231213 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1096/fj.201900382RR |
| PMID: | 31560576 |
| قاعدة البيانات: | MEDLINE |
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