Editorial & Opinion
Characterizations and Fibrinolytic Activity of Serine Protease from Bacillus subtilis C10.
| العنوان: | Characterizations and Fibrinolytic Activity of Serine Protease from Bacillus subtilis C10. |
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| المؤلفون: | Thu NTA; Institute of Bioactive Compounds and Department of Biology, University of Sciences, Hue University, Hue, Vietnam.; University of Medicine and Pharmacy, Hue University, Hue, Vietnam; 3Institute of Biotechnology, Hue University, Vietnam., Khue NTM; Institute of Bioactive Compounds and Department of Biology, University of Sciences, Hue University, Hue, Vietnam., Huy ND; Institute of Biotechnology, Hue University, Vietnam., Tien NQD; Institute of Bioactive Compounds and Department of Biology, University of Sciences, Hue University, Hue, Vietnam., Loc NH; Institute of Bioactive Compounds and Department of Biology, University of Sciences, Hue University, Hue, Vietnam. |
| المصدر: | Current pharmaceutical biotechnology [Curr Pharm Biotechnol] 2020; Vol. 21 (2), pp. 110-116. |
| نوع المنشور: | Letter |
| اللغة: | English |
| بيانات الدورية: | Publisher: Bentham Science Publishers Country of Publication: Netherlands NLM ID: 100960530 Publication Model: Print Cited Medium: Internet ISSN: 1873-4316 (Electronic) Linking ISSN: 13892010 NLM ISO Abbreviation: Curr Pharm Biotechnol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Hilversum, Netherlands ; Boca Raton, FL : Bentham Science Publishers, c2000- |
| مواضيع طبية MeSH: | Bacillus subtilis/*enzymology , Fibrinolytic Agents/*pharmacology , Serine Proteases/*pharmacology, Animal Shells/microbiology ; Animals ; Fibrinolytic Agents/isolation & purification ; Hydrogen-Ion Concentration ; Molecular Weight ; Penaeidae/microbiology ; Serine Proteases/isolation & purification ; Temperature |
| مستخلص: | Background: Fibrinolytic enzymes, such as Nattokinases from Bacillus species are known to degrade the fibrin blood clots. They belong to serine protease group having commercial applications, such as therapeutic agents and functional food formulation. Objective: The present study reports some characteristics and fibrinolytic activity of serine protease from B. subtilis C10 strain that was isolated from shrimp shell. Methods: Extracellular enzyme from B. subtilis C10 culture was harvested and partially purified by ammonium sulphate precipitation. Fibrinolytic activity of the enzyme was determined by zymography and measured by spectrophotometry with fibrinogen and thrombin used as substrates. The optimal temperature and pH for fibrinolytic activity were studied in the range of 31-43ºC and 5-10, respectively. The thermal and pH stability of enzyme was studied by incubating enzyme for 30 min in the same range of temperature and pH as above. The effect of some metal ions and reagents on fibrinolytic activity of enzyme was evaluated by concentrations of 5 mM and 5%, respectively. Results: Zymogram analysis indicated the presence of four fibrinolytic enzymes with molecular weights of approximately 69, 67, 39 and 36 kDa. The optimal temperature and pH for enzyme activity were 37°C and 9, respectively. The thermal and pH stability ranged from 35-39°C and 8-10, respectively. Fibrinolytic activity reached a maximum value of about 400 U/mg protein after 16 h of C10 strain culture. Enzyme has been drastically inhibited by PMSF and SDS, and partially inhibited by EDTA, while Triton X-100 has significantly increased enzyme activity. Effects of ions such as Mg2+, Ca2+ and Mn2+ on enzyme were negligible, except Cu2+ and Zn2+ have strongly decreased its activity. Conclusion: Results from the present study suggested that enzyme obtained from B. subtilis C10 could be serine protease that has a high fibrinolytic activity up to about 400 U/mg protein at the most appropriate temperature and pH of 37ºC and 9. This activity can be improved up to 142% by incubating enzyme with 5% Triton X-100 for 30 min. (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.) |
| فهرسة مساهمة: | Keywords: Bacillus subtilis; enzyme production; fibrinolytic activity; nattokinase; serine protease; zymogram. |
| المشرفين على المادة: | 0 (Fibrinolytic Agents) EC 3.4.- (Serine Proteases) |
| تواريخ الأحداث: | Date Created: 20191003 Date Completed: 20200501 Latest Revision: 20200501 |
| رمز التحديث: | 20221213 |
| DOI: | 10.2174/1389201020666191002145415 |
| PMID: | 31577203 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1873-4316 |
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| DOI: | 10.2174/1389201020666191002145415 |