التفاصيل البيبلوغرافية
| العنوان: |
Effects of Papain-Based Gel Used For Caries Removal on Macrophages and Dental Pulp Cells. |
| المؤلفون: |
Bastos LA; Department of Pediatric Clinics, USP - Universidade de São Paulo, Ribeirão Preto, SP, Brazil., Silva FL; Department of Pediatric Clinics, USP - Universidade de São Paulo, Ribeirão Preto, SP, Brazil., Thomé JPQ; FACISB - Faculdade de Ciências da Saúde de Barretos Dr. Paulo Prata, Barretos, SP, Brazil., Arnez MFM; Department of Pediatric Clinics, USP - Universidade de São Paulo, Ribeirão Preto, SP, Brazil., Faccioli LH; Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Laboratório de Inflamação e Imunologia das Parasitoses, Faculdade USP -Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Ribeirão Preto, SP, Brazil., Paula-Silva FWG; Department of Pediatric Clinics, USP - Universidade de São Paulo, Ribeirão Preto, SP, Brazil.; Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Laboratório de Inflamação e Imunologia das Parasitoses, Faculdade USP -Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Ribeirão Preto, SP, Brazil. |
| المصدر: |
Brazilian dental journal [Braz Dent J] 2019 Oct 07; Vol. 30 (5), pp. 484-490. Date of Electronic Publication: 2019 Oct 07 (Print Publication: 2019). |
| نوع المنشور: |
Journal Article |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: Dental Foundation of Ribeirão Preto Country of Publication: Brazil NLM ID: 9214652 Publication Model: eCollection Cited Medium: Internet ISSN: 1806-4760 (Electronic) Linking ISSN: 01036440 NLM ISO Abbreviation: Braz Dent J |
| أسماء مطبوعة: |
Original Publication: Ribeirão Preto : Dental Foundation of Ribeirão Preto, [1990- |
| مواضيع طبية MeSH: |
Dental Caries* , Papain*, Dental Cavity Preparation ; Dental Pulp ; Humans ; Macrophages |
| مستخلص: |
Papain-based gel is used for chemical-mechanical caries removal and present antimicrobial and anti-inflammatory activities. However, its effects on dental pulp cells and on macrophages remains largely unknown. Therefore, the aim of this study was to investigate whether the papain-based gel Papacárie Duo® acts as an immunomodulator in lipopolysaccharide (LPS)-activated macrophages and its effects on dental pulp cells . J774.1 macrophage and OD-21 dental pulp cells were stimulated with 0.5% and 5% of Papacárie Duo®, following pre-treatment or not with LPS. After 24 h, a lactate dehydrogenase assay was used to measure cytotoxicity, a tetrazolium-based colorimetric assay (MTT) was used to measure cell viability, and qRT-PCR was used to analyze relative gene expression of Ptgs2, Il10, Tnf, Mmp9, Runx2, Ibsp and Spp1. Papacárie Duo® was cytotoxic and reduced cell viability at 5% but not at 0.5% in both cultures. In macrophages, Papacárie Duo® increased the expression Il10 and LPS-induced Ptgs2, but it did not affect Tnf or Mmp9. In OD-21 cells, Papacárie Duo® inhibited Runx2 and Ibsp expression, but stimulated Spp1 expression. Papain-based gel presented a concentration dependent cytotoxicity, without affecting cell viability, for dental pulp cells and macrophages. Interestingly, the gel presented an inhibitory effect on pulp cell differentiation but modulated the activation of macrophages stimulated with LPS. We speculate that in dental pulp tissue, Papacárie Duo® would impair reparative dentinogenesis but could activate macrophages to perform their role in defense and inflammation. |
| المشرفين على المادة: |
EC 3.4.22.2 (Papain) |
| تواريخ الأحداث: |
Date Created: 20191010 Date Completed: 20191022 Latest Revision: 20191022 |
| رمز التحديث: |
20240628 |
| DOI: |
10.1590/0103-6440201902560 |
| PMID: |
31596333 |
| قاعدة البيانات: |
MEDLINE |
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