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Transcriptomics of Tasmanian Devil ( Sarcophilus Harrisii ) Ear Tissue Reveals Homogeneous Gene Expression Patterns across a Heterogeneous Landscape.

التفاصيل البيبلوغرافية
العنوان: Transcriptomics of Tasmanian Devil ( Sarcophilus Harrisii ) Ear Tissue Reveals Homogeneous Gene Expression Patterns across a Heterogeneous Landscape.
المؤلفون: Fraik AK; School of Biological Sciences, Washington State University, Pullman, WA 99164, USA. alexandra.fraik@wsu.edu., Quackenbush C; School of Biological Sciences, Washington State University, Pullman, WA 99164, USA. corey.quackenbush@wsu.edu., Margres MJ; School of Biological Sciences, Washington State University, Pullman, WA 99164, USA. mark.margres@wsu.edu.; Department of Biological Sciences, Clemson University, Clemson, SC 29634, USA. mark.margres@wsu.edu., Comte S; School of Natural Sciences, Hobart, TAS 7001, Australia. sebastien.comte@utas.edu.au.; Vertebrate Pest Research Unit, NSW Department of Primary Industries, 1447 Forest Road, Orange, NSW 2800, Australia. sebastien.comte@utas.edu.au., Hamilton DG; School of Natural Sciences, Hobart, TAS 7001, Australia. d.g.hamilton@utas.edu.au., Kozakiewicz CP; School of Biological Sciences, Washington State University, Pullman, WA 99164, USA. chris.kozakiewicz@wsu.edu., Jones M; School of Natural Sciences, Hobart, TAS 7001, Australia. menna.jones@utas.edu.au., Hamede R; School of Natural Sciences, Hobart, TAS 7001, Australia. rodrigo.hamedeross@utas.edu.au., Hohenlohe PA; Department of Biological Sciences, University of Idaho, Institute for Bioinformatics and Evolutionary Studies, University of Idaho, 875 Perimeter Drive, Moscow, ID 83844, USA. hohenlohe@uidaho.edu., Storfer A; Department of Biological Sciences, University of Idaho, Institute for Bioinformatics and Evolutionary Studies, University of Idaho, 875 Perimeter Drive, Moscow, ID 83844, USA. astorfer@wsu.edu., Kelley JL; Department of Biological Sciences, University of Idaho, Institute for Bioinformatics and Evolutionary Studies, University of Idaho, 875 Perimeter Drive, Moscow, ID 83844, USA. joanna.l.kelley@wsu.edu.
المصدر: Genes [Genes (Basel)] 2019 Oct 12; Vol. 10 (10). Date of Electronic Publication: 2019 Oct 12.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101551097 Publication Model: Electronic Cited Medium: Internet ISSN: 2073-4425 (Electronic) Linking ISSN: 20734425 NLM ISO Abbreviation: Genes (Basel) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel : MDPI
مواضيع طبية MeSH: Genetic Variation*, Marsupialia/*genetics , Transcriptome/*genetics, Adaptation, Biological/genetics ; Animals ; Cluster Analysis ; Computational Biology ; Ear ; Endangered Species ; Female ; Gene Expression Regulation ; Gene Ontology ; Male ; Population/genetics ; RNA-Seq
مستخلص: In an era of unprecedented global change, exploring patterns of gene expression among wild populations across their geographic range is crucial for characterizing adaptive potential. RNA-sequencing studies have successfully characterized gene expression differences among populations experiencing divergent environmental conditions in a wide variety of taxa. However, few of these studies have identified transcriptomic signatures to multivariate, environmental stimuli among populations in their natural environments. Herein, we aim to identify environmental and sex-driven patterns of gene expression in the Tasmanian devil ( Sarcophilus harrisii ), a critically endangered species that occupies a heterogeneous environment. We performed RNA-sequencing on ear tissue biopsies from adult male and female devils from three populations at the extremes of their geographic range. There were no transcriptome-wide patterns of differential gene expression that would be suggestive of significant, environmentally-driven transcriptomic responses. The general lack of transcriptome-wide variation in gene expression levels across the devil's geographic range is consistent with previous studies that documented low levels of genetic variation in the species. However, genes previously implicated in local adaptation to abiotic environment in devils were enriched for differentially expressed genes. Additionally, three modules of co-expressed genes were significantly associated with either population of origin or sex.
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معلومات مُعتمدة: R01 GM126563 United States GM NIGMS NIH HHS; R01-GM12563 United States NH NIH HHS
فهرسة مساهمة: Keywords: RNA-sequencing; Tasmanian devil; conservation genomics; geographic variation; population level; sex-specific expression
تواريخ الأحداث: Date Created: 20191017 Date Completed: 20200226 Latest Revision: 20231014
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC6826840
DOI: 10.3390/genes10100801
PMID: 31614864
قاعدة البيانات: MEDLINE
الوصف
تدمد:2073-4425
DOI:10.3390/genes10100801