دورية أكاديمية
Receptor Heterodimerization and Co-Receptor Engagement in TLR2 Activation Induced by MIC1 and MIC4 from Toxoplasma gondii .
| العنوان: | Receptor Heterodimerization and Co-Receptor Engagement in TLR2 Activation Induced by MIC1 and MIC4 from Toxoplasma gondii . |
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| المؤلفون: | Costa Mendonça-Natividade F; Laboratory of Immunochemistry and Glycobiology, Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo (FMRP/USP), Ribeirão Preto SP 14049-900, Brazil. flavia.bioquimica@usp.br., Duque Lopes C; Laboratory of Immunochemistry and Glycobiology, Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo (FMRP/USP), Ribeirão Preto SP 14049-900, Brazil. cduquelopes@gmail.com., Ricci-Azevedo R; Laboratory of Immunochemistry and Glycobiology, Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo (FMRP/USP), Ribeirão Preto SP 14049-900, Brazil. rrazevedo@gmail.com., Sardinha-Silva A; Laboratory of Immunochemistry and Glycobiology, Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo (FMRP/USP), Ribeirão Preto SP 14049-900, Brazil. aline.sardinhadasilva@nih.gov., Figueiredo Pinzan C; Laboratory of Immunochemistry and Glycobiology, Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo (FMRP/USP), Ribeirão Preto SP 14049-900, Brazil. capinzan@gmail.com., Paiva Alegre-Maller AC; Laboratory of Immunochemistry and Glycobiology, Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo (FMRP/USP), Ribeirão Preto SP 14049-900, Brazil. anapaiva@fag.edu.br., L Nohara L; Border Biomedical Research Center (BBRC), Department of Biological Sciences, University of Texas at El Paso (UTEP), El Paso, TX 79968, USA. llnohara@msl.ubc.ca., B Carneiro A; Border Biomedical Research Center (BBRC), Department of Biological Sciences, University of Texas at El Paso (UTEP), El Paso, TX 79968, USA. alan.fiocruz@gmail.com.; Institute of Medical Biochemistry, Program of Molecular Biology and Biotechnology at Federal University of Rio de Janeiro (UFRJ), Rio de Janeiro RJ 21941-599, Brazil. alan.fiocruz@gmail.com., Panunto-Castelo A; Department of Biology, Faculty of Philosophy, Sciences and Letters at Ribeirão Preto, University of São Paulo USP (FFCLRP/USP), Ribeirão Preto SP 14040-900, Brazil. apcastelo@usp.br., C Almeida I; Border Biomedical Research Center (BBRC), Department of Biological Sciences, University of Texas at El Paso (UTEP), El Paso, TX 79968, USA. icalmeida@utep.edu., Roque-Barreira MC; Laboratory of Immunochemistry and Glycobiology, Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo (FMRP/USP), Ribeirão Preto SP 14049-900, Brazil. mcrbarre@fmrp.usp.br. |
| المصدر: | International journal of molecular sciences [Int J Mol Sci] 2019 Oct 10; Vol. 20 (20). Date of Electronic Publication: 2019 Oct 10. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, [2000- |
| مواضيع طبية MeSH: | Dimerization*, Cell Adhesion Molecules/*chemistry , Cell Adhesion Molecules/*metabolism , Protozoan Proteins/*chemistry , Protozoan Proteins/*metabolism , Toll-Like Receptor 2/*chemistry , Toll-Like Receptor 2/*metabolism , Toxoplasma/*metabolism, Animals ; CD36 Antigens/genetics ; CD36 Antigens/metabolism ; Cytokines/analysis ; Female ; Gene Knockdown Techniques ; HEK293 Cells ; Humans ; Lipopolysaccharide Receptors/genetics ; Lipopolysaccharide Receptors/metabolism ; MAP Kinase Kinase Kinases/metabolism ; Macrophages/metabolism ; Mice ; Mice, Inbred C57BL ; NF-kappa B/metabolism ; RAW 264.7 Cells ; Signal Transduction ; Toll-Like Receptor 1/metabolism ; Toll-Like Receptor 6/metabolism ; p38 Mitogen-Activated Protein Kinases/metabolism |
| مستخلص: | The microneme organelles of Toxoplasma gondii tachyzoites release protein complexes (MICs), including one composed of the transmembrane protein MIC6 plus MIC1 and MIC4. In this complex, carbohydrate recognition domains of MIC1 and MIC4 are exposed and interact with terminal sialic acid and galactose residues, respectively, of host cell glycans. Recently, we demonstrated that MIC1 and MIC4 binding to the N-glycans of Toll-like receptor (TLR) 2 and TLR4 on phagocytes triggers cell activation and pro-inflammatory cytokine production. Herein, we investigated the requirement for TLR2 heterodimerization and co-receptors in MIC-induced responses, as well as the signaling molecules involved. We used MICs to stimulate macrophages and HEK293T cells transfected with TLR2 and TLR1 or TLR6, both with or without the co-receptors CD14 and CD36. Then, the cell responses were analyzed, including nuclear factor-kappa B (NF-κB) activation and cytokine production, which showed that (1) only TLR2, among the studied factors, is crucial for MIC-induced cell activation; (2) TLR2 heterodimerization augments, but is not critical for, activation; (3) CD14 and CD36 enhance the response to MIC stimulus; and (4) MICs activate cells through a transforming growth factor beta-activated kinase 1 (TAK1)-, mammalian p38 mitogen-activated protein kinase (p38)-, and NF-κB-dependent pathway. Remarkably, among the studied factors, the interaction of MIC1 and MIC4 with TLR2 N -glycans is sufficient to induce cell activation, which promotes host protection against T. gondii infection. |
| References: | Glycobiology. 2001 Jul;11(7):541-7. (PMID: 11447133) Trends Immunol. 2013 Jul;34(7):307-16. (PMID: 23664135) Eur J Biochem. 2001 Aug;268(16):4580-9. (PMID: 11502220) PLoS One. 2014 Jun 03;9(6):e98512. (PMID: 24892697) Trends Immunol. 2011 Mar;32(3):104-9. (PMID: 21269878) J Innate Immun. 2014;6(5):685-94. (PMID: 24861338) J Immunol. 1994 Aug 15;153(4):1798-807. (PMID: 8046246) Cell. 2007 Sep 21;130(6):1071-82. (PMID: 17889651) Nat Immunol. 2010 Feb;11(2):155-61. (PMID: 20037584) FEMS Microbiol Rev. 2012 May;36(3):717-33. (PMID: 22091606) J Leukoc Biol. 2013 Nov;94(5):885-902. (PMID: 23990624) PLoS One. 2009 Oct 22;4(10):e7411. (PMID: 19847289) Vaccine. 2013 Jun 28;31(31):3135-9. (PMID: 23707448) PLoS One. 2007 Aug 29;2(8):e788. (PMID: 17726518) Nat Rev Immunol. 2014 Aug;14(8):546-58. (PMID: 25060580) PLoS One. 2013 Sep 30;8(9):e76233. (PMID: 24312681) Nat Rev Immunol. 2007 May;7(5):353-64. (PMID: 17457343) Nat Protoc. 2010 May;5(5):929-34. (PMID: 20431538) J Biol Chem. 1999 Nov 19;274(47):33419-25. (PMID: 10559223) Science. 2005 Jun 10;308(5728):1626-9. (PMID: 15860593) Nat Rev Immunol. 2014 Feb;14(2):109-21. (PMID: 24457485) Org Biomol Chem. 2009 Sep 21;7(18):3629-38. (PMID: 19707662) Front Immunol. 2014 Sep 25;5:461. (PMID: 25309543) Int Immunol. 2001 Jul;13(7):933-40. (PMID: 11431423) Oncogene. 2007 May 14;26(22):3214-26. (PMID: 17496917) Glycobiology. 2001 Dec;11(12):1035-42. (PMID: 11805076) J Biol Chem. 2004 Aug 13;279(33):34589-94. (PMID: 15173186) EMBO J. 2007 Jun 6;26(11):2808-20. (PMID: 17491595) FEMS Microbiol Lett. 1998 Jul 15;164(2):323-7. (PMID: 9682481) Proc Natl Acad Sci U S A. 2000 Dec 5;97(25):13766-71. (PMID: 11095740) Nat Rev Immunol. 2013 Jun;13(6):453-60. (PMID: 23681101) PLoS Negl Trop Dis. 2016 Aug 10;10(8):e0004848. (PMID: 27508930) Nat Immunol. 2000 Nov;1(5):398-401. (PMID: 11062499) Nat Immunol. 2010 May;11(5):373-84. (PMID: 20404851) Proc Natl Acad Sci U S A. 2010 Feb 9;107(6):2634-9. (PMID: 20133807) Microbes Infect. 2006 Apr;8(5):1244-51. (PMID: 16616574) Vaccine. 2009 Nov 5;27(47):6570-4. (PMID: 19720368) J Exp Med. 2005 Feb 7;201(3):453-63. (PMID: 15684324) Parasitol Res. 2013 Aug;112(8):2871-7. (PMID: 23749087) Infect Immun. 2000 Jul;68(7):4005-11. (PMID: 10858215) Org Biomol Chem. 2016 Feb 14;14(6):1930-3. (PMID: 26786502) Nature. 2005 Feb 3;433(7025):523-7. (PMID: 15690042) Cold Spring Harb Symp Quant Biol. 1989;54 Pt 1:1-13. (PMID: 2700931) J Cell Biol. 2001 Feb 5;152(3):563-78. (PMID: 11157983) J Biol Chem. 2013 May 17;288(20):14178-88. (PMID: 23548899) Phytomedicine. 2011 Feb 15;18(4):309-16. (PMID: 20724126) Curr Top Microbiol Immunol. 2002;270:121-44. (PMID: 12467248) J Leukoc Biol. 2008 Jul;84(1):280-91. (PMID: 18458151) Nat Rev Immunol. 2012 Feb 03;12(3):168-79. (PMID: 22301850) Annu Rev Biochem. 2011;80:917-41. (PMID: 21548780) PLoS Pathog. 2019 Jun 21;15(6):e1007871. (PMID: 31226171) J Cell Biol. 2002 May 13;157(4):557-63. (PMID: 12011107) Science. 2012 Feb 17;335(6070):859-64. (PMID: 22344444) J Biol Chem. 2004 Sep 24;279(39):40882-9. (PMID: 15247273) Front Immunol. 2017 Dec 18;8:1820. (PMID: 29326706) J Biol Chem. 1999 Jan 15;274(3):1267-76. (PMID: 9880495) Sci Rep. 2017 Aug 1;7(1):7083. (PMID: 28765651) Inflamm Res. 2000 Dec;49(12):732-6. (PMID: 11211926) Cell Mol Immunol. 2014 Sep;11(5):477-94. (PMID: 24769793) J Biol Chem. 2005 Nov 18;280(46):38583-91. (PMID: 16166092) Evid Based Complement Alternat Med. 2011;2011:298972. (PMID: 19939951) Int J Parasitol. 2010 Aug 15;40(10):1145-54. (PMID: 20430033) Clin Microbiol Rev. 2012 Apr;25(2):264-96. (PMID: 22491772) Cell Host Microbe. 2013 Jan 16;13(1):42-53. (PMID: 23290966) Chem Soc Rev. 2013 Jun 7;42(11):4798-813. (PMID: 23353569) J Immunol. 2000 Jun 1;164(11):5564-74. (PMID: 10820230) Eur J Cell Biol. 1997 Jun;73(2):114-23. (PMID: 9208224) PLoS Negl Trop Dis. 2015 Dec 02;9(12):e0004238. (PMID: 26630499) J Biol Chem. 2010 Jan 15;285(3):2064-76. (PMID: 19901027) J Clin Invest. 2008 Jan;118(1):342-51. (PMID: 18064303) PLoS Negl Trop Dis. 2014 Dec 04;8(12):e3317. (PMID: 25474158) Science. 2008 Apr 18;320(5874):379-81. (PMID: 18420935) J Biol Chem. 2001 Feb 9;276(6):4119-27. (PMID: 11053441) PLoS One. 2015 Nov 17;10(11):e0143087. (PMID: 26575028) Adv Exp Med Biol. 2007;598:389-406. (PMID: 17892226) PLoS Pathog. 2014 Jun 12;10(6):e1004120. (PMID: 24945143) J Biol Chem. 2006 Oct 13;281(41):31002-11. (PMID: 16880211) Cell Microbiol. 2006 Dec;8(12):1899-909. (PMID: 16848791) Immunol Lett. 2008 Aug 15;119(1-2):17-21. (PMID: 18617274) Immunity. 2013 Jan 24;38(1):119-30. (PMID: 23246311) J Biol Chem. 2012 May 11;287(20):16720-33. (PMID: 22399295) J Immunol. 2007 Jul 15;179(2):1129-37. (PMID: 17617606) Parasit Vectors. 2015 Jul 22;8:384. (PMID: 26194005) Mol Immunol. 2015 Feb;63(2):143-52. (PMID: 24951397) Lancet. 2004 Jun 12;363(9425):1965-76. (PMID: 15194258) J Biol Chem. 2002 Jan 18;277(3):1845-54. (PMID: 11706042) PLoS One. 2010 Dec 17;5(12):e15263. (PMID: 21179419) Parasitol Res. 2010 Apr;106(5):1079-84. (PMID: 20177910) Immunity. 2002 Mar;16(3):429-39. (PMID: 11911827) Nat Med. 2003 May;9(5):525-32. (PMID: 12692544) Nat Rev Immunol. 2017 Sep;17(9):545-558. (PMID: 28580957) Trends Parasitol. 2011 Sep;27(9):388-93. (PMID: 21550851) Vet Parasitol. 2007 Sep 1;148(2):93-101. (PMID: 17651898) Epidemiol Infect. 2015 Feb;143(3):600-7. (PMID: 24850323) |
| معلومات مُعتمدة: | #2013/04088-0; #2014/13324-1; 2008/52130-7;2017/02998-0 Fundação de Amparo à Pesquisa do Estado de São Paulo; #475357/2013-2;#306298/2013-9 Conselho Nacional de Desenvolvimento Científico e Tecnológico; G12MD007592;2G12MD007592 United States MD NIMHD NIH HHS |
| فهرسة مساهمة: | Keywords: CD14; CD36; TLR co-receptors; TLR heterodimerization; Toll-like receptor 2; Toxoplasma gondii; microneme proteins |
| المشرفين على المادة: | 0 (CD14 protein, human) 0 (CD36 Antigens) 0 (CD36 protein, human) 0 (Cell Adhesion Molecules) 0 (Cytokines) 0 (Lipopolysaccharide Receptors) 0 (MIC1 protein, Toxoplasma gondii) 0 (MIC4 protein, Toxoplasma gondii) 0 (NF-kappa B) 0 (Protozoan Proteins) 0 (TLR1 protein, human) 0 (TLR2 protein, human) 0 (TLR6 protein, human) 0 (Toll-Like Receptor 1) 0 (Toll-Like Receptor 2) 0 (Toll-Like Receptor 6) EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases) EC 2.7.11.25 (MAP Kinase Kinase Kinases) EC 2.7.11.25 (MAP kinase kinase kinase 7) |
| تواريخ الأحداث: | Date Created: 20191030 Date Completed: 20200331 Latest Revision: 20200331 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC6829480 |
| DOI: | 10.3390/ijms20205001 |
| PMID: | 31658592 |
| قاعدة البيانات: | MEDLINE |
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