دورية أكاديمية
Influence of different extenders on morphological and functional parameters of frozen-thawed spermatozoa of jaguar (Panthera onca).
| العنوان: | Influence of different extenders on morphological and functional parameters of frozen-thawed spermatozoa of jaguar (Panthera onca). |
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| المؤلفون: | Silva HVR; Laboratory of Carnivores Reproduction, State University of Ceará, Fortaleza, CE, Brazil., Nunes TGP; Laboratory of Carnivores Reproduction, State University of Ceará, Fortaleza, CE, Brazil., Brito BF; Laboratory of Carnivores Reproduction, State University of Ceará, Fortaleza, CE, Brazil., Campos LB; Laboratory of Animal Germplasm Conservation, Federal Rural University of Semi-Arid, Mossoró, RN, Brazil., Silva AMD; Laboratory of Animal Germplasm Conservation, Federal Rural University of Semi-Arid, Mossoró, RN, Brazil., Silva AR; Laboratory of Animal Germplasm Conservation, Federal Rural University of Semi-Arid, Mossoró, RN, Brazil., Comizzoli P; Smithsonian Conservation Biology Institute, Washington, USA., Silva LDMD; Laboratory of Carnivores Reproduction, State University of Ceará, Fortaleza, CE, Brazil. Electronic address: lucia.daniel.machado@hotmail.com. |
| المصدر: | Cryobiology [Cryobiology] 2020 Feb 01; Vol. 92, pp. 53-61. Date of Electronic Publication: 2019 Nov 05. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0006252 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2392 (Electronic) Linking ISSN: 00112240 NLM ISO Abbreviation: Cryobiology Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2000- > : Amsterdam : Elsevier Original Publication: San Diego, CA : Academic Press |
| مواضيع طبية MeSH: | Cryoprotective Agents/*pharmacology , Panthera/*embryology , Semen Preservation/*methods , Spermatozoa/*ultrastructure , Tromethamine/*pharmacology, Animals ; Cocos/chemistry ; Cryopreservation/methods ; Cryoprotective Agents/chemistry ; Egg Yolk/chemistry ; Freezing ; Humans ; Male ; Microscopy, Electron, Transmission ; Semen/physiology ; Semen Analysis ; Sperm Motility |
| مستخلص: | Due to the global decrease in jaguar population, conservation strategies are essential and the development of effective semen cryopreservation protocols would contribute to the formation of germplasm banks. Therefore, the objectives were to (1) evaluate the use of TRIS and ACP-117c extenders for jaguar semen freezing, (2) describe the ultrastructural changes in sperm after cryopreservation, and (3) evaluate the binding capacity of the thawed sperm. Eight ejaculates from five mature individuals were collected by electroejaculation, extended in TRIS or a coconut based-extender (ACP-117c), and frozen in liquid nitrogen. Samples were evaluated for sperm motility, vigor, membrane functionality, mitochondrial activity, morphology (using light microscopy, scanning electron microscopy - SEM and transmission electron microscopy - TEM), sperm kinetic parameters (by computerized analysis - CASA), and sperm binding capability using an egg yolk perivitelline membrane assay. Samples preserved in TRIS presented better post-thaw motility (46.0 ± 7.7%) and membrane functionality (60.5 ± 4.2%) and higher mitochondrial activity (21.5 ± 3.7%) than those preserved in ACP-117c (20.9 ± 5.4% motile sperm; 47.1 ± 2.5% functional membrane; 11.8 ± 1.7% mitochondrial activity). Regarding ultrastructural evaluations, SEM showed that both extenders were able to preserve the superficial membrane of the sperm, but TEM revealed the occurrence of nuclear electron lucent points, especially in samples extended in ACP-117c. Additionally, TRIS also provided a higher number of sperm bound to the perivitelline membrane (29.5 ± 3.3%) in comparison to samples diluted in ACP-117c (18.6 ± 1.5%). Overall, we suggest the use of a TRIS-based extender for cryopreservation of jaguar semen. Competing Interests: Declaration of competing interest The authors have stated that there are no competing interests. None of the authors has financial or personal relationships that may influence or distort the content of the article. (Copyright © 2019 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: ACP-117c; Felids; Frozen semen; Perivitelline membrane test; Sperm ultrastructure; TRIS; Wildlife |
| المشرفين على المادة: | 0 (Cryoprotective Agents) 023C2WHX2V (Tromethamine) |
| تواريخ الأحداث: | Date Created: 20191110 Date Completed: 20201013 Latest Revision: 20201013 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.cryobiol.2019.10.195 |
| PMID: | 31704199 |
| قاعدة البيانات: | MEDLINE |
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