دورية أكاديمية
Detection of Schistosoma japonicum and Oncomelania hupensis quadrasi environmental DNA and its potential utility to schistosomiasis japonica surveillance in the Philippines.
| العنوان: | Detection of Schistosoma japonicum and Oncomelania hupensis quadrasi environmental DNA and its potential utility to schistosomiasis japonica surveillance in the Philippines. |
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| المؤلفون: | Fornillos RJC; DNA Barcoding Laboratory, Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City, Philippines.; Natural Sciences Research Institute, University of the Philippines Diliman, P. Velasquez St. Diliman, Quezon City, Philippines., Sato MO; Department of Tropical Medicine and Parasitology, Dokkyo Medical University, Tochigi, Japan., Tabios IKB; College of Medicine, University of the Philippines Manila, Ermita Manilla, Philippines., Sato M; Graduate School of Health Sciences, Niigata, Japan., Leonardo LR; DNA Barcoding Laboratory, Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City, Philippines.; Graduate School, University of the East Ramon Magsaysay Memorial Medical Center, Quezon City, Philippines., Chigusa Y; Department of Tropical Medicine and Parasitology, Dokkyo Medical University, Tochigi, Japan., Minamoto T; Graduate School of Human Development and Environment, Kobe University, Tsurukabuto, Nada-ku, Kobe, Japan., Kikuchi M; Department of Immunogenetics, Institute of Tropical Medicine, Nagasaki University, Sakamoto, Nagasaki, Japan., Legaspi ER; Medical Zoology Laboratory, Schistosomiasis Research and Training Center, Palo Leyte, Philippines., Fontanilla IKC; DNA Barcoding Laboratory, Institute of Biology, College of Science, University of the Philippines Diliman, Quezon City, Philippines.; Natural Sciences Research Institute, University of the Philippines Diliman, P. Velasquez St. Diliman, Quezon City, Philippines. |
| المصدر: | PloS one [PLoS One] 2019 Nov 20; Vol. 14 (11), pp. e0224617. Date of Electronic Publication: 2019 Nov 20 (Print Publication: 2019). |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: San Francisco, CA : Public Library of Science |
| مواضيع طبية MeSH: | Epidemiological Monitoring*, DNA, Environmental/*isolation & purification , Schistosoma japonicum/*isolation & purification , Schistosomiasis japonica/*prevention & control , Snails/*genetics, Animals ; Cercaria/genetics ; DNA, Environmental/genetics ; Disease Vectors ; Humans ; Philippines/epidemiology ; Schistosoma japonicum/genetics ; Schistosomiasis japonica/epidemiology ; Schistosomiasis japonica/parasitology ; Schistosomiasis japonica/transmission ; Snails/parasitology ; Species Specificity |
| مستخلص: | In recent years, the prevalence and infection intensity of Schistosoma japonicum in endemic areas of the Philippines have significantly decreased due to yearly population-based treatment strategies, yet transmission rates remain high and uninterrupted. An important indicator of active disease transmission is the presence of Schistosoma japonicum and its snail intermediate host Oncomelania hupensis quadrasi in freshwater habitats. In this study, we sought to apply a species-specific real-time PCR (qPCR) assay for the detection of S. japonicum and O. hupensis quadrasi in freshwater samples using environmental DNA approach that can complement the commonly utilized malacological survey in determining potential transmission foci in order to have a more effective snail surveillance strategy for schistosomiasis japonica in endemic areas. The newly developed assay was specific to S. japonicum and O. hupensis quadrasi with no amplification detected against non-target trematode Fasciola spp. and snails such as Lymnaea spp., Pomacea canaliculata, and Melanoides spp. that typically co-exist in the same environment. The assay effectiveness was determined using 19 environmental water samples collected from Northern Samar (N = 5 sites), Leyte (N = 11 sites) and Compostela Valley (N = 3 sites) and compared to malacological survey for determining O. hupensis quadrasi snail colonies and snail crushing to visualize S. japonicum cercariae. TaqMan qPCR targeting a short fragment of the cytochrome c oxidase subunit 1 (cox1) gene was positive for S. japonicum in 9 sites, for O. hupensis quadrasi in 9 sites, and for both S. japonicum and O. hupensis quadrasi in 5 sampling sites. Moreover, it was able to detect O. hupensis quadrasi in 3 out of 12 sites found negative and 6 out of 7 sites found positive through malacological survey, and in 4 of the 5 snail sites positive for snails with cercariae. Overall, this method can complement malacological surveys for monitoring of schistosomes in endemic areas of the Philippines, especially those with high risk of human infection. Competing Interests: The authors have declared that no competing interests exist. |
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| المشرفين على المادة: | 0 (DNA, Environmental) |
| تواريخ الأحداث: | Date Created: 20191121 Date Completed: 20200402 Latest Revision: 20231104 |
| رمز التحديث: | 20231104 |
| مُعرف محوري في PubMed: | PMC6867693 |
| DOI: | 10.1371/journal.pone.0224617 |
| PMID: | 31747401 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1932-6203 |
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| DOI: | 10.1371/journal.pone.0224617 |