دورية أكاديمية
Live Cell Monitoring and Enrichment of Stem Cell-Derived β Cells Using Intracellular Zinc Content as a Population Marker.
| العنوان: | Live Cell Monitoring and Enrichment of Stem Cell-Derived β Cells Using Intracellular Zinc Content as a Population Marker. |
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| المؤلفون: | Davis JC; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, Massachusetts.; Harvard Stem Cell Institute, Harvard University, Cambridge, Massachusetts., Helman A; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, Massachusetts.; Harvard Stem Cell Institute, Harvard University, Cambridge, Massachusetts., Rivera-Feliciano J; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, Massachusetts.; Harvard Stem Cell Institute, Harvard University, Cambridge, Massachusetts., Langston CM; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, Massachusetts.; Harvard Stem Cell Institute, Harvard University, Cambridge, Massachusetts., Engquist EN; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, Massachusetts.; Harvard Stem Cell Institute, Harvard University, Cambridge, Massachusetts., Melton DA; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, Massachusetts.; Harvard Stem Cell Institute, Harvard University, Cambridge, Massachusetts.; Howard Hughes Medical Institute, Chevy Chase, Maryland. |
| المصدر: | Current protocols in stem cell biology [Curr Protoc Stem Cell Biol] 2019 Dec; Vol. 51 (1), pp. e99. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Country of Publication: United States NLM ID: 101470226 Publication Model: Print Cited Medium: Internet ISSN: 1938-8969 (Electronic) Linking ISSN: 19388969 NLM ISO Abbreviation: Curr Protoc Stem Cell Biol Subsets: MEDLINE |
| مواضيع طبية MeSH: | Insulin-Secreting Cells/*cytology , Staining and Labeling/*methods , Zinc/*metabolism, Cell Culture Techniques/methods ; Humans |
| مستخلص: | Our laboratory and others have developed protocols to generate glucose-responsive stem cell-derived β cells in vitro. The cells resulting from these protocols could supplement or replace the use of human cadaveric islets for cell-based therapy for diabetes. The combination of an unlimited supply of pluripotent stem cell-derived β cells and gene-editing approaches will facilitate numerous in vitro studies not possible with cadaveric islets. Here, we describe a protocol for fluorescent labeling and isolation of stem cell-derived β cells. This purification of SC-β cells is based on intracellular zinc content and is a simple method to complement other approaches for generating and assaying these cells. © 2019 The Authors. Basic Protocol: Fluorescent labeling and isolation of stem cell-derived β cells. (© 2019 The Authors.) |
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| معلومات مُعتمدة: | UC4 DK104159 United States DK NIDDK NIH HHS; UC4 DK104165 United States DK NIDDK NIH HHS; United States HHMI Howard Hughes Medical Institute |
| فهرسة مساهمة: | Keywords: SC-β cell enrichment; intracellular zinc content; stem cell-derived β (SC-β) cell |
| المشرفين على المادة: | J41CSQ7QDS (Zinc) |
| تواريخ الأحداث: | Date Created: 20191123 Date Completed: 20200909 Latest Revision: 20200909 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC6876704 |
| DOI: | 10.1002/cpsc.99 |
| PMID: | 31756031 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1938-8969 |
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| DOI: | 10.1002/cpsc.99 |