دورية أكاديمية
أعرض في EDS

Type I-F CRISPR-Cas resistance against virulent phages results in abortive infection and provides population-level immunity.

التفاصيل البيبلوغرافية
العنوان: Type I-F CRISPR-Cas resistance against virulent phages results in abortive infection and provides population-level immunity.
المؤلفون: Watson BNJ; Department of Microbiology and Immunology, University of Otago, Dunedin, 9054, New Zealand.; ESI, Biosciences, University of Exeter, Cornwall Campus, Penryn, TR10 9FE, UK., Vercoe RB; Department of Microbiology and Immunology, University of Otago, Dunedin, 9054, New Zealand., Salmond GPC; Department of Biochemistry, University of Cambridge, Cambridge, CB2 1QW, UK., Westra ER; ESI, Biosciences, University of Exeter, Cornwall Campus, Penryn, TR10 9FE, UK., Staals RHJ; Department of Microbiology and Immunology, University of Otago, Dunedin, 9054, New Zealand.; Laboratory of Microbiology, Wageningen University and Research, 6708, WE, Wageningen, The Netherlands., Fineran PC; Department of Microbiology and Immunology, University of Otago, Dunedin, 9054, New Zealand. peter.fineran@otago.ac.nz.; Bio-Protection Research Centre, University of Otago, Dunedin, New Zealand. peter.fineran@otago.ac.nz.
المصدر: Nature communications [Nat Commun] 2019 Dec 04; Vol. 10 (1), pp. 5526. Date of Electronic Publication: 2019 Dec 04.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: Bacteria/*immunology , Bacteriophages/*immunology , CRISPR-Cas Systems/*immunology , Pectobacterium/*immunology, Bacteria/genetics ; Bacteria/virology ; Bacterial Infections/immunology ; Bacterial Infections/microbiology ; Bacterial Infections/virology ; Bacteriophages/genetics ; Bacteriophages/physiology ; CRISPR-Cas Systems/genetics ; Microbial Viability/genetics ; Microbial Viability/immunology ; Pectobacterium/genetics ; Pectobacterium/virology ; Virus Replication/genetics ; Virus Replication/immunology
مستخلص: Type I CRISPR-Cas systems are abundant and widespread adaptive immune systems in bacteria and can greatly enhance bacterial survival in the face of phage infection. Upon phage infection, some CRISPR-Cas immune responses result in bacterial dormancy or slowed growth, which suggests the outcomes for infected cells may vary between systems. Here we demonstrate that type I CRISPR immunity of Pectobacterium atrosepticum leads to suppression of two unrelated virulent phages, ɸTE and ɸM1. Immunity results in an abortive infection response, where infected cells do not survive, but viral propagation is severely decreased, resulting in population protection due to the reduced phage epidemic. Our findings challenge the view of CRISPR-Cas as a system that protects the individual cell and supports growing evidence of abortive infection by some types of CRISPR-Cas systems.
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معلومات مُعتمدة: BB/K001833/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; BB/N008081/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council
تواريخ الأحداث: Date Created: 20191205 Date Completed: 20200309 Latest Revision: 20231020
رمز التحديث: 20231020
مُعرف محوري في PubMed: PMC6892833
DOI: 10.1038/s41467-019-13445-2
PMID: 31797922
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/s41467-019-13445-2