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Molecular method for rapid detection of the red tide dinoflagellate Karenia mikimotoi in the coastal region of Xiangshan Bay, China.

التفاصيل البيبلوغرافية
العنوان: Molecular method for rapid detection of the red tide dinoflagellate Karenia mikimotoi in the coastal region of Xiangshan Bay, China.
المؤلفون: Huang HL; Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ningbo 315211, China; Key Laboratory of Marine Ecology & Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. Electronic address: huanghailong@qdio.ac.cn., Gao WF; Ningbo Institute of Oceanography, Ningbo 315832, China. Electronic address: 1411085869@nbu.edu.cn., Zhu P; Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ningbo 315211, China; Ningbo Institute of Oceanography, Ningbo 315832, China. Electronic address: zhupengnbu@nbu.edu.cn., Zhou CX; Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ningbo 315211, China. Electronic address: zhouchengxu@nbu.edu.cn., Qiao LL; Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ningbo 315211, China., Dang CY; Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ningbo 315211, China., Pang JH; Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ningbo 315211, China., Yan XJ; Key Laboratory of Applied Marine Biotechnology, Ningbo University, Ningbo 315211, China. Electronic address: yanxiaojun@nbu.edu.cn.
المصدر: Journal of microbiological methods [J Microbiol Methods] 2020 Jan; Vol. 168, pp. 105801. Date of Electronic Publication: 2019 Dec 04.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Biomedical Country of Publication: Netherlands NLM ID: 8306883 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-8359 (Electronic) Linking ISSN: 01677012 NLM ISO Abbreviation: J Microbiol Methods Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam, The Netherlands : Elsevier Biomedical, c1983-
مواضيع طبية MeSH: Chromatography/*methods , Dinoflagellida/*isolation & purification , Molecular Diagnostic Techniques/*methods , Nucleic Acid Amplification Techniques/*methods, Bays ; China ; Chromatography/instrumentation ; DNA Primers/genetics ; DNA, Intergenic/genetics ; Dinoflagellida/genetics ; Harmful Algal Bloom ; Sensitivity and Specificity
مستخلص: The species Karenia mikimotoi is a common nearshore red tide alga that can secrete hemolytic exotoxin and ichthyotoxin, which can induce the death of fish and shellfish, causing severe economic losses. In this study, loop-mediated isothermal amplification (LAMP) was employed in combination with the lateral flow dipstick (LFD) visual detection method to establish the LAMP-LFD rapid detection method for K. mikimotoi. The internal transcribed spacer ITS1-5.8S-ITS2 of K. mikimotoi was used as the target sequence and was amplified with specific primers designed in this study. The results indicated that the amplification optimal reaction conditions for LAMP in this paper were for 20 min at 65 °C. Moreover, LAMP had excellent specificity, showing negative results for other common red tide causing algal species. In field samples, we successfully reduced the total time, with only 23 min needed from LAMP amplification to LFD result display, which was shorter than that of conventional PCR. Consequently, LAMP-LFD should be useful for rapid field detection of low-density K. mikimotoi and for the early prevention of red tide induced by such algae.
(Copyright © 2019. Published by Elsevier B.V.)
فهرسة مساهمة: Keywords: Harmful algae; ITS; Karenia mikimotoi; LAMP-LFD assay; Rapid
المشرفين على المادة: 0 (DNA Primers)
0 (DNA, Intergenic)
SCR Protocol: LAMP assay
تواريخ الأحداث: Date Created: 20191208 Date Completed: 20210212 Latest Revision: 20210212
رمز التحديث: 20240628
DOI: 10.1016/j.mimet.2019.105801
PMID: 31811904
قاعدة البيانات: MEDLINE
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