دورية أكاديمية
أعرض في EDS

A mathematical modeling approach to the quantification of lactic acid bacteria in vacuum-packaged samples of cooked meat: Combining the TaqMan-based quantitative PCR method with the plate-count method.

التفاصيل البيبلوغرافية
العنوان: A mathematical modeling approach to the quantification of lactic acid bacteria in vacuum-packaged samples of cooked meat: Combining the TaqMan-based quantitative PCR method with the plate-count method.
المؤلفون: Martins WF; Federal University of Santa Catarina, Department of Chemical Engineering and Food Engineering, Center of Technology, Florianópolis, SC 88040-901, Brazil; Federal Institute of Education, Science and Technology of Goiano, Food Technology, Campus Morrinhos, Morrinhos, GO 75650-000, Brazil., Longhi DA; Federal University of Paraná, Food Engineering, Campus Jandaia do Sul, Jandaia do Sul, PR 86900-000, Brazil., de Aragão GMF; Federal University of Santa Catarina, Department of Chemical Engineering and Food Engineering, Center of Technology, Florianópolis, SC 88040-901, Brazil., Melero B; University of Burgos, Department of Biotechnology and Food Science, Burgos 09001, Spain., Rovira J; University of Burgos, Department of Biotechnology and Food Science, Burgos 09001, Spain., Diez AM; University of Burgos, Department of Biotechnology and Food Science, Burgos 09001, Spain. Electronic address: amdiez@ubu.es.
المصدر: International journal of food microbiology [Int J Food Microbiol] 2020 Apr 02; Vol. 318, pp. 108466. Date of Electronic Publication: 2019 Dec 02.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Publishers Country of Publication: Netherlands NLM ID: 8412849 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-3460 (Electronic) Linking ISSN: 01681605 NLM ISO Abbreviation: Int J Food Microbiol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam : Elsevier Science Publishers, c1984-
مواضيع طبية MeSH: Models, Theoretical*, Food Microbiology/*methods , Food Packaging/*methods , Lactobacillales/*growth & development , Meat Products/*microbiology, Animals ; Colony Count, Microbial ; Real-Time Polymerase Chain Reaction ; Temperature ; Vacuum
مستخلص: The TaqMan-based quantitative Polymerase Chain Reaction (qPCR) method and the Plate Count (PC) method are both used in combination with primary and secondary mathematical modeling, to describe the growth curves of Leuconostoc mesenteroides and Weissella viridescens in vacuum-packaged meat products during storage under different isothermal conditions. Vacuum-Packaged Morcilla (VPM), a typical cooked blood sausage, is used as a representative meat product, with the aim of improving shelf-life prediction methods for those sorts of meat products. The standard curves constructed by qPCR showed good linearity between the cycle threshold (C T ) and log 10 CFU/g, demonstrating the high precision and the reproducible results of the qPCR method. The curves were used for the quantification of L. mesenteroides and W. viridescens in artificially inoculated VPM samples under isothermal storage (5, 8, 13 and 18 °C). Primally, both the qPCR and the PC methods were compared, and a linear regression analysis demonstrated a statistically significant linear correlation between the methods. Secondly, the Baranyi and Roberts model was fitted to the growth curve data to estimate the kinetic parameters of L. mesenteroides and W. viridescens under isothermal conditions, and secondary models were used to establish the dependence of the maximum specific growth rate on the temperature. The results proved that primary and secondary models were adequate for describing the growth curves of both methods in relation to both bacteria. In conclusion, the results of all the experiments proved that the qPCR method in combination with the PC method can be used to construct microbial growth kinetics and that primary and secondary mathematical modeling can be successfully applied to describe the growth of L. mesenteroides and W. viridescens in vacuum-packaged morcilla and, by extension, other cooked meat products with similar characteristics.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2019 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Leuconostoc mesenteroides; Mathematical modeling; Shelf-life; Weissella viridescens; qPCR
تواريخ الأحداث: Date Created: 20191223 Date Completed: 20200529 Latest Revision: 20200529
رمز التحديث: 20231215
DOI: 10.1016/j.ijfoodmicro.2019.108466
PMID: 31865245
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-3460
DOI:10.1016/j.ijfoodmicro.2019.108466