دورية أكاديمية
أعرض في EDS

Bioassay- and metabolomics-guided screening of bioactive soil actinomycetes from the ancient city of Ihnasia, Egypt.

التفاصيل البيبلوغرافية
العنوان: Bioassay- and metabolomics-guided screening of bioactive soil actinomycetes from the ancient city of Ihnasia, Egypt.
المؤلفون: Sebak M; Strathclyde Institute of Pharmacy and Biomedical Sciences, Faculty of Science, University of Strathclyde, Glasgow, Scotland, United Kingdom.; Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Menoufia University, Shebin Elkom, Menoufia, Egypt., Saafan AE; Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Menoufia University, Shebin Elkom, Menoufia, Egypt.; Microbiology and Immunology Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt., AbdelGhani S; Microbiology and Immunology Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt., Bakeer W; Microbiology and Immunology Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt., El-Gendy AO; Microbiology and Immunology Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt., Espriu LC; Strathclyde Institute of Pharmacy and Biomedical Sciences, Faculty of Science, University of Strathclyde, Glasgow, Scotland, United Kingdom., Duncan K; Strathclyde Institute of Pharmacy and Biomedical Sciences, Faculty of Science, University of Strathclyde, Glasgow, Scotland, United Kingdom., Edrada-Ebel R; Strathclyde Institute of Pharmacy and Biomedical Sciences, Faculty of Science, University of Strathclyde, Glasgow, Scotland, United Kingdom.
المصدر: PloS one [PLoS One] 2019 Dec 30; Vol. 14 (12), pp. e0226959. Date of Electronic Publication: 2019 Dec 30 (Print Publication: 2019).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Soil Microbiology*, Actinobacteria/*isolation & purification , Anti-Infective Agents/*isolation & purification, Actinobacteria/chemistry ; Actinobacteria/metabolism ; Anti-Infective Agents/pharmacology ; Biological Assay ; Biological Products/isolation & purification ; Biological Products/pharmacology ; Egypt ; Inhibitory Concentration 50 ; Metabolomics ; Microbial Sensitivity Tests
مستخلص: Literature surveys, taxonomical differences, and bioassay results have been utilized in the discovery of new natural products to aid in Actinomycetes isolate-selection. However, no or less investigation have been done on establishing the differences in metabolomic profiles of the isolated microorganisms. The study aims to utilise bioassay- and metabolomics-guided tools that included dereplication study and multivariate analysis of the NMR and mass spectral data of microbial extracts to assist the selection of isolates for scaling-up the production of antimicrobial natural products. A total of 58 actinomycetes were isolated from different soil samples collected from Ihnasia City, Egypt and screened for their antimicrobial activities against indicator strains that included Bacillus subtilis, Escherichia coli, methicillin-resistant Staphylococcus aureus and Candida albicans. A number of 25 isolates were found to be active against B. subtilis and/or to at least one of the tested indicator strains. Principal component analyses showed chemical uniqueness for four outlying bioactive actinomycetes extracts. In addition, Orthogonal Projections to Latent Structures Discriminant Analysis (OPLS-DA) and dereplication study led us to further select two outlying anti-MRSA active isolates MS.REE.13 and 22 for scale-up work. MS.REE.13 and 22 exhibited zones of inhibition at 19 and 13 mm against MRSA, respectively. A metabolomics-guided approach provided the steer to target the bioactive metabolites (P<0.01) present in a crude extract or fraction even at nanogram levels but it was a challenge that such low-yielding bioactive natural products would be feasible to isolate. Validated to occur only on the active side of OPLS-DA loadings plot, the isolated compounds exhibited medium to weak antibiotic activity with MIC values between 250 and 800 μM. Two new compounds, P_24306 (C10H13N2) and N_12799 (C18H32O3) with MICs of 795 and 432 μM, were afforded from the scale-up of MS.REE. 13 and 22, respectively.
Competing Interests: The authors have declared that no competing interests exist.
التعليقات: Erratum in: PLoS One. 2020 Feb 3;15(2):e0228901. (PMID: 32012209)
References: Nat Biotechnol. 2012 Oct;30(10):918-20. (PMID: 23051804)
J Bacteriol. 1991 Jan;173(2):697-703. (PMID: 1987160)
J Ind Microbiol Biotechnol. 2005 Jun;32(6):234-52. (PMID: 15895265)
Appl Environ Microbiol. 2007 Feb;73(4):1146-52. (PMID: 17158611)
Methods Mol Biol. 2013;1055:227-44. (PMID: 23963915)
Curr Opin Biotechnol. 2010 Dec;21(6):780-6. (PMID: 20956080)
J Antibiot (Tokyo). 1969 Dec;22(12):629-34. (PMID: 5367395)
J Nat Prod. 2011 Oct 28;74(10):2219-23. (PMID: 21958359)
Planta Med. 2015 Apr;81(6):450-8. (PMID: 25615275)
J Nat Prod. 2013 Sep 27;76(9):1686-99. (PMID: 24025162)
Saudi J Biol Sci. 2018 Jan;25(1):44-46. (PMID: 29379355)
Int J Syst Evol Microbiol. 2007 Sep;57(Pt 9):2009-13. (PMID: 17766864)
Acc Chem Res. 2012 Feb 21;45(2):288-97. (PMID: 21888316)
J Mol Evol. 1980 Dec;16(2):111-20. (PMID: 7463489)
Metabolites. 2015 Jul 08;5(3):404-30. (PMID: 26184333)
Chem Biodivers. 2017 Oct;14(10):null. (PMID: 28672096)
Appl Microbiol. 1957 Jan;5(1):25-33. (PMID: 13403634)
J Antibiot (Tokyo). 2005 Jan;58(1):1-26. (PMID: 15813176)
Fitoterapia. 2015 Apr;102:203-7. (PMID: 25655350)
Sci Rep. 2019 Feb 22;9(1):2547. (PMID: 30796274)
Nature. 1965 Apr 17;206(981):263-5. (PMID: 5840561)
Antibiotiki. 1971 Jul;16(7):579-83. (PMID: 5168223)
Mar Drugs. 2014 Jun 02;12(6):3323-51. (PMID: 24893324)
J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Feb 1;1106-1107:71-83. (PMID: 30658264)
Front Microbiol. 2016 Jul 19;7:1135. (PMID: 27486455)
Mar Drugs. 2014 Jun 05;12(6):3416-48. (PMID: 24905482)
Nat Prod Rep. 2014 Mar;31(3):381-99. (PMID: 24496105)
Front Microbiol. 2018 Apr 18;9:687. (PMID: 29720968)
J Nat Prod. 2012 Mar 23;75(3):311-35. (PMID: 22316239)
Chem Pharm Bull (Tokyo). 1975 Oct;23(10):2201-10. (PMID: 1212749)
J Ind Microbiol Biotechnol. 2003 Aug;30(8):472-9. (PMID: 12687492)
Br J Cancer. 2008 Jan 15;98(1):154-60. (PMID: 18087280)
Nat Protoc. 2010 Mar;5(3):479-90. (PMID: 20203665)
PLoS One. 2015 Sep 25;10(9):e0138528. (PMID: 26407167)
Antimicrob Agents Chemother. 2012 Jan;56(1):110-4. (PMID: 22064543)
Indian J Pharm Sci. 2009 Sep;71(5):499-504. (PMID: 20502566)
Open Microbiol J. 2009;3:53-7. (PMID: 19440253)
Mol Biol Evol. 1987 Jul;4(4):406-25. (PMID: 3447015)
Carbohydr Res. 2008 Jul 21;343(10-11):1636-43. (PMID: 18468589)
Front Microbiol. 2015 Nov 26;6:1316. (PMID: 26635777)
Anal Chim Acta. 2013 Dec 17;805:70-9. (PMID: 24296145)
Antimicrob Agents Chemother. 1975 Mar;7(3):349-63. (PMID: 1137388)
J Ind Microbiol Biotechnol. 2000 Dec;25(6):342-345. (PMID: 11320422)
Antimicrob Agents Chemother. 1977 May;11(5):852-7. (PMID: 879738)
Microbiol Rev. 1987 Jun;51(2):221-71. (PMID: 2439888)
Nat Rev Drug Discov. 2015 Feb;14(2):111-29. (PMID: 25614221)
Trends Microbiol. 2007 Jun;15(6):279-89. (PMID: 17433686)
Mar Drugs. 2014 Mar 06;12(3):1220-44. (PMID: 24663112)
Antimicrob Agents Chemother. 2006 Jun;50(6):1946-52. (PMID: 16723550)
Curr Genomics. 2011 Sep;12(6):391-403. (PMID: 22379393)
J Am Chem Soc. 2017 Sep 20;139(37):12939-12942. (PMID: 28853876)
J Gen Microbiol. 1983 Jun;129(6):1815-30. (PMID: 6688823)
Anal Chem. 2012 May 15;84(10):4277-83. (PMID: 22519562)
Annu Rev Microbiol. 1983;37:189-216. (PMID: 6357051)
Chem Biol. 1998 Oct;5(10):R245-9. (PMID: 9818143)
Mol Biol Evol. 2007 Aug;24(8):1596-9. (PMID: 17488738)
Front Microbiol. 2017 Mar 13;8:438. (PMID: 28348553)
Nucleic Acids Res. 2015 Jan;43(Database issue):D30-5. (PMID: 25414350)
Mol Biol Evol. 2016 Jul;33(7):1870-4. (PMID: 27004904)
Molecules. 2018 Dec 11;23(12):null. (PMID: 30544987)
Int J Syst Evol Microbiol. 2004 Jan;54(Pt 1):203-9. (PMID: 14742481)
J Nat Prod. 2011 Oct 28;74(10):2278-81. (PMID: 21967034)
Environ Sci Technol. 2009 Oct 15;43(20):7658-64. (PMID: 19921875)
J Antibiot (Tokyo). 1987 Oct;40(10):1455-6. (PMID: 3680012)
J Antibiot (Tokyo). 2009 Jan;62(1):5-16. (PMID: 19132062)
J Biotechnol. 2002 May 9;95(2):133-44. (PMID: 11911923)
Planta Med. 2017 Apr;83(6):565-573. (PMID: 27760442)
BMC Bioinformatics. 2010 Jul 23;11:395. (PMID: 20650010)
Planta Med. 2018 Feb;84(3):182-190. (PMID: 28847019)
المشرفين على المادة: 0 (Anti-Infective Agents)
0 (Biological Products)
تواريخ الأحداث: Date Created: 20191231 Date Completed: 20200409 Latest Revision: 20200409
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC6936774
DOI: 10.1371/journal.pone.0226959
PMID: 31887193
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0226959