دورية أكاديمية
Molecular and genomic characterization of a novel equine molluscum contagiosum-like virus.
| العنوان: | Molecular and genomic characterization of a novel equine molluscum contagiosum-like virus. |
|---|---|
| المؤلفون: | Ehmann R; Bundeswehr Institute of Microbiology, Munich, Germany., Brandes K; Animal Pathology Augsburg, Augsburg, Germany., Antwerpen M; Bundeswehr Institute of Microbiology, Munich, Germany., Walter M; Bundeswehr Institute of Microbiology, Munich, Germany., V Schlippenbach K; Veterinarian Practice, Zusamaltheim, Germany., Stegmaier E; Makoa Farm, Uswaa, Tanzania., Essbauer S; Bundeswehr Institute of Microbiology, Munich, Germany., Bugert J; Bundeswehr Institute of Microbiology, Munich, Germany., Teifke JP; Federal Research Institute for Animal Health, Greifswald - Insel Riems, Germany., Meyer H; Bundeswehr Institute of Microbiology, Munich, Germany. |
| المصدر: | The Journal of general virology [J Gen Virol] 2021 Mar; Vol. 102 (3). Date of Electronic Publication: 2020 Jan 10. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Microbiology Society Country of Publication: England NLM ID: 0077340 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1465-2099 (Electronic) Linking ISSN: 00221317 NLM ISO Abbreviation: J Gen Virol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : London : Microbiology Society Original Publication: London, Cambridge Univ. Press for the Society for General Microbiology. |
| مواضيع طبية MeSH: | Genome, Viral*, Horse Diseases/*virology , Molluscipoxvirus/*genetics , Molluscipoxvirus/*physiology , Poxviridae Infections/*veterinary , Skin Diseases, Viral/*veterinary , Viral Proteins/*genetics, Animals ; Female ; High-Throughput Nucleotide Sequencing ; Horses ; Intercellular Signaling Peptides and Proteins/chemistry ; Intercellular Signaling Peptides and Proteins/genetics ; Intercellular Signaling Peptides and Proteins/metabolism ; Membrane Proteins/chemistry ; Membrane Proteins/genetics ; Membrane Proteins/metabolism ; Molluscipoxvirus/isolation & purification ; Molluscum contagiosum virus/genetics ; Open Reading Frames ; Phylogeny ; Poxviridae Infections/pathology ; Poxviridae Infections/virology ; Skin/pathology ; Skin/virology ; Skin Diseases, Viral/pathology ; Skin Diseases, Viral/virology ; Transcription, Genetic ; Viral Proteins/chemistry ; Viral Proteins/metabolism ; Virus Replication/genetics ; Whole Genome Sequencing |
| مستخلص: | Cases of pox-like lesions in horses and donkeys have been associated with poxviruses belonging to different genera of the family Poxviridae . These include the orthopoxviruses vaccinia virus (VACV), horsepoxvirus (HPXV) and cowpoxvirus (CPXV), as well as a potentially novel parapoxvirus and molluscum contagiosum virus (MOCV). However, with the exception of VACV, HPXV and CPXV, the genomic characterization of the causative agents remains largely elusive with only single short genome fragments available. Here we present the first full-length genome sequence of an equine molluscum contagiosum-like virus (EMCLV) directly determined from skin biopsies of a horse with generalized papular dermatitis. Histopathological analysis of the lesions revealed severe epidermal hyperplasia with numerous eosinophilic inclusion bodies within keratinocytes. Virions were detected in the lesions in embedded tissue by transmission electron microscopy. The genome sequence determined by next- and third-generation sequencing comprises 166 843 nt with inverted terminal repeats (ITRs) of 3473 nt. Overall, 20 of the predicted 159 ORFs have no equivalents in other poxviruses. Intriguingly, two of these ORFs were identified to encode homologues of mammalian proteins involved in immune signalling pathways, namely secreted and transmembrane protein 1 (SECTM1) and insulin growth factor-like family receptor 1 (IGFLR1), that were not described in any virus family so far. Phylogenetic analysis with all relevant representatives of the Poxviridae suggests that EMCLV should be nominated as a new species within the genus Molluscipoxvirus . |
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| فهرسة مساهمة: | Keywords: IGFLR1; SECTM1; equine molluscum; novel poxvirus, Molluscipoxvirus |
| المشرفين على المادة: | 0 (Intercellular Signaling Peptides and Proteins) 0 (Membrane Proteins) 0 (Viral Proteins) 0 (interleukin-18 binding protein) |
| تواريخ الأحداث: | Date Created: 20200111 Date Completed: 20210716 Latest Revision: 20211016 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC8515872 |
| DOI: | 10.1099/jgv.0.001357 |
| PMID: | 31922947 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1465-2099 |
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| DOI: | 10.1099/jgv.0.001357 |