دورية أكاديمية
Studying Xenograft Rejection of Bioprosthetic Heart Valves.
| العنوان: | Studying Xenograft Rejection of Bioprosthetic Heart Valves. |
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| المؤلفون: | Manji RA; Department of Surgery, University of Manitoba, Winnipeg, MB, Canada. rmanji@sbgh.mb.ca.; Cardiac Sciences Program, I.H. Asper Clinical Research Institute, Winnipeg Regional Health Authority and St. Boniface Hospital, Winnipeg, MB, Canada. rmanji@sbgh.mb.ca., Manji JS; Cardiac Sciences Program, I.H. Asper Clinical Research Institute, Winnipeg Regional Health Authority and St. Boniface Hospital, Winnipeg, MB, Canada. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2020; Vol. 2110, pp. 227-243. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Heart Valve Prosthesis*, Graft Rejection/*immunology , Heart Valves/*transplantation , Heterografts/*immunology , Transplantation, Heterologous/*adverse effects , Transplantation, Heterologous/*methods, Animals ; Antibodies, Heterophile/immunology ; Biomarkers ; Biopsy ; Enzyme-Linked Immunosorbent Assay ; Graft Rejection/metabolism ; Graft Rejection/pathology ; Graft Survival/immunology ; Guinea Pigs ; Immunoglobulin G/immunology ; Postoperative Care ; Rats |
| مستخلص: | Millions of patients with valvular heart disease have benefitted from heart valve replacement since the procedure was first introduced in the 1960s; however, there are still many patients who get early structural valve deterioration (SVD) of their bioprosthetic heart valves (BHV). BHV are porcine, bovine, or equine tissues that have been glutaraldehyde fixed to preserve the tissue and presumably make the tissue immunologically inert. These glutaraldehyde-fixed BHV with anti-calcification treatments last long periods of time in older adults but develop early SVD in younger patients. The consensus at present is that the early SVD in younger patients is due to more "wear and tear" of the valves and higher calcium turnover in younger patients. However, as younger patients likely have a more robust immune system than older adults, there is a new hypothesis that BHV xenografts may undergo xenograft rejection, and this may contribute to the early SVD seen in younger patients.At present, the technology to noninvasively study in vivo whether an implanted BHV in a human patient is undergoing rejection is not available. Thus, a small animal discordant xenotransplant model in young rodents (to match the young patient getting a pig/bovine/equine BHV) was developed to study whether the hypothesis that glutaraldehyde-fixed BHV undergo xenograft rejection had any merit. In this chapter, we describe our model and its merits and the results of our investigations. Our work provides clear evidence of xenograft rejection in glutaraldehyde-fixed tissue, and our small animal model offers an opportunity to study this process in detail. |
| فهرسة مساهمة: | Keywords: Bioprosthetic heart valve; Calcification; Cardiac surgery; Glutaraldehyde; Xenograft rejection; Young patient |
| المشرفين على المادة: | 0 (Antibodies, Heterophile) 0 (Biomarkers) 0 (Immunoglobulin G) |
| تواريخ الأحداث: | Date Created: 20200201 Date Completed: 20210127 Latest Revision: 20210127 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1007/978-1-0716-0255-3_15 |
| PMID: | 32002912 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-0716-0255-3_15 |