دورية أكاديمية
Production and characterization of a monoclonal antibody to the trichothecene mycotoxin diacetoxyscirpenol.
| العنوان: | Production and characterization of a monoclonal antibody to the trichothecene mycotoxin diacetoxyscirpenol. |
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| المؤلفون: | Pauly JU; Institut für Medizinische Mikrobiologie, Universität Mainz., Bitter-Suermann D, Dose K |
| المصدر: | Biological chemistry Hoppe-Seyler [Biol Chem Hoppe Seyler] 1988 Jun; Vol. 369 (6), pp. 487-92. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Walter De Gruyter Country of Publication: Germany NLM ID: 8503054 Publication Model: Print Cited Medium: Print ISSN: 0177-3593 (Print) Linking ISSN: 01773593 NLM ISO Abbreviation: Biol Chem Hoppe Seyler Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Berlin : Walter De Gruyter Original Publication: Berlin ; New York : W. de Gruyter, c1985-c1996. |
| مواضيع طبية MeSH: | Antibodies, Monoclonal*, Mycotoxins/*immunology , Sesquiterpenes/*immunology , Trichothecenes/*immunology, Animals ; Cross Reactions ; Enzyme-Linked Immunosorbent Assay ; Female ; Mice ; Mice, Inbred BALB C ; Trichothecenes/analysis |
| مستخلص: | A monoclonal antibody was obtained by the fusion of mouse myeloma cells with splenocytes isolated from Balb/c mice, which had been immunized with diacetoxyscirpenol-hemiglutarate (DAS-hemiglutarate) and verrucarol-hemiglutarates covalently bound to ethylenediamine-modified bovine serum albumin. The anti-DAS-antibody that could be induced was of the IgM type with kappa-chains. The titer of the monoclonal anti-DAS-antibody in ascites fluid obtained from mice injected the selected cell line was much higher than those of conventional antisera. An enzyme-linked immunosorbent assay based on the competitive binding principle in which the antibody was applied had a sensitivity of 1 ng DAS per assay. The relative cross-reactivity of the monoclonal antibody in the CI-ELISA with the related trichothecenes such as triacetoxyscirpenol, 15-monoacetoxyscirpenol, diacetylverrucarol, 4-monoacetoxyscirpenol and scirpentriol were found to be 1.8, 0.8, 0.15, 0.02 and less than 0.001, respectively. The trichothecenes verrucarol, T-2 toxin, T-2 tetraol, deoxynivalenol, 3-acetyldeoxynivalenol and trichothecin showed no cross-reactivity. |
| المشرفين على المادة: | 0 (Antibodies, Monoclonal) 0 (Mycotoxins) 0 (Sesquiterpenes) 0 (Trichothecenes) UYL28I099N (diacetoxyscirpenol) |
| تواريخ الأحداث: | Date Created: 19880601 Date Completed: 19890209 Latest Revision: 20190919 |
| رمز التحديث: | 20221208 |
| DOI: | 10.1515/bchm3.1988.369.1.487 |
| PMID: | 3202955 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0177-3593 |
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| DOI: | 10.1515/bchm3.1988.369.1.487 |