دورية أكاديمية
أعرض في EDS

Recombinant elongation factor 1 alpha of Haemonchus contortus affects the functions of goat PBMCs.

التفاصيل البيبلوغرافية
العنوان: Recombinant elongation factor 1 alpha of Haemonchus contortus affects the functions of goat PBMCs.
المؤلفون: Ehsan M; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.; State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China., Gadahi JA; Department of Veterinary Parasitology, Sindh Agriculture University, Tandojam, Pakistan., Lu M; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China., Yan R; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China., Xu L; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China., Song X; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China., Zhu XQ; State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China., Du A; College of Animal Sciences, Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, China., Hu M; State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China., Li X; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
المصدر: Parasite immunology [Parasite Immunol] 2020 May; Vol. 42 (5), pp. e12703. Date of Electronic Publication: 2020 Feb 28.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Wiley Country of Publication: England NLM ID: 7910948 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1365-3024 (Electronic) Linking ISSN: 01419838 NLM ISO Abbreviation: Parasite Immunol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Oxford, Wiley
مواضيع طبية MeSH: Goat Diseases/*parasitology , Haemonchiasis/*veterinary , Haemonchus/*immunology , Helminth Proteins/*immunology , Leukocytes, Mononuclear/*immunology , Peptide Elongation Factor 1/*immunology, Animals ; Apoptosis ; Cell Movement ; Cell Proliferation ; Goat Diseases/genetics ; Goat Diseases/immunology ; Goat Diseases/physiopathology ; Goats ; Haemonchiasis/immunology ; Haemonchiasis/parasitology ; Haemonchiasis/physiopathology ; Haemonchus/genetics ; Helminth Proteins/genetics ; Interleukin-17/genetics ; Interleukin-17/immunology ; Nitric Oxide/immunology ; Peptide Elongation Factor 1/genetics
مستخلص: Excretory/secretory proteins of Haemonchus contortus (HcESPs) intermingle comprehensively with host immune cells and modulate host immune responses. In this study, H contortus ES antigen named as elongation factor 1 alpha (HcEF-1α) was cloned and expressed. The influences of recombinant HcEF-1α on multiple functions of goat peripheral blood mononuclear cells (PBMCs) were observed in vitro. Immunoblot analysis revealed that rHcEF-1α was recognized by the serum of goat infected with H contortus. Immunofluorescence analysis indicated that rHcEF-1α was bound on surface of PBMCs. Moreover, the productions of IL-4, TGF-β1, IFN-γ and IL-17 of cells were significantly modulated by the incubation with rHcEF-1α. The production of interleukin IL-10 was decreased. Cell migration, cell proliferation and cell apoptosis were significantly increased; however, nitric oxide production (NO) was significantly decreased. The MHC II molecule expression of cells incubated with rHcEF-1α was increased significantly, whereas MHC-I was not changed as compared to the control groups (PBS control and pET32a). These findings indicated that rHcEF-1α protein might play essential roles in functional regulations of HcESPs on goat PBMC and mediate the immune responses of the host during host-parasite relationship.
(© 2020 The Authors. Parasite Immunology published by John Wiley & Sons Ltd.)
References: Science. 2007 Jan 12;315(5809):207-12. (PMID: 17218520)
Nature. 2004 Oct 28;431(7012):1107-12. (PMID: 15510150)
J Biol Chem. 1994 Sep 16;269(37):23045-50. (PMID: 8083206)
Parasit Vectors. 2017 Jun 26;10(1):311. (PMID: 28651566)
Vet Parasitol. 2011 Sep 27;181(2-4):239-47. (PMID: 21570191)
Vet Parasitol. 2017 Aug 30;243:100-104. (PMID: 28807275)
Histochem Cell Biol. 2013 Dec;140(6):603-9. (PMID: 23839781)
J Immunol. 2011 Dec 15;187(12):6417-27. (PMID: 22079980)
Parasitology. 2013 Oct;140(12):1506-22. (PMID: 23998513)
Scand J Urol Nephrol. 2010 Nov;44(5):277-83. (PMID: 20545466)
Mol Genet Genomics. 2009 Apr;281(4):391-405. (PMID: 19132394)
Oncotarget. 2017 Nov 26;8(68):112211-112221. (PMID: 29348819)
Nature. 1990 Oct 4;347(6292):494-6. (PMID: 2215665)
Front Microbiol. 2017 Feb 13;8:168. (PMID: 28243226)
Int J Parasitol. 2001 Dec;31(14):1563-71. (PMID: 11730782)
Plant Physiol Biochem. 2007 Feb;45(2):113-8. (PMID: 17344053)
PLoS Pathog. 2014 Apr 10;10(4):e1004047. (PMID: 24722202)
Trends Biochem Sci. 1990 Nov;15(11):420-4. (PMID: 2278101)
Mol Biochem Parasitol. 2012 May;183(1):15-22. (PMID: 22261090)
Anal Biochem. 1976 May 7;72:248-54. (PMID: 942051)
J Biol Chem. 2013 Nov 22;288(47):34111-20. (PMID: 24085304)
Vet Immunol Immunopathol. 2008 Mar 15;122(1-2):138-45. (PMID: 18187208)
Wiad Parazytol. 2005;51(4):271-80. (PMID: 16913499)
Parasite Immunol. 2020 May;42(5):e12703. (PMID: 32043596)
Parasit Vectors. 2015 Apr 09;8:211. (PMID: 25879191)
Res Vet Sci. 2012 Apr;92(2):259-65. (PMID: 21444098)
Nature. 1991 Jan 10;349(6305):117-27. (PMID: 1898771)
Mycol Res. 2005 Feb;109(Pt 2):173-86. (PMID: 15839101)
Mol Cell Biochem. 2006 Jun;286(1-2):17-22. (PMID: 16652225)
Oncotarget. 2016 Dec 20;7(51):83926-83937. (PMID: 27893414)
Parasitol Res. 2007 Nov;101(6):1557-62. (PMID: 17674047)
Exp Cell Res. 1998 Jan 10;238(1):168-76. (PMID: 9457069)
Adv Exp Med Biol. 2014;841:99-151. (PMID: 25261206)
Biochem J. 2015 Feb 15;466(1):69-76. (PMID: 25423367)
PLoS One. 2013 Jun 14;8(6):e66436. (PMID: 23799104)
Crit Rev Immunol. 1993;13(3-4):247-68. (PMID: 8110378)
Parasite Immunol. 2007 Mar;29(3):139-51. (PMID: 17266741)
Int J Parasitol. 2006 Jul;36(8):859-68. (PMID: 16759659)
Eur J Immunol. 1990 Dec;20(12):2651-9. (PMID: 2269329)
Parasit Vectors. 2014 Jul 23;7:342. (PMID: 25056558)
Parasite Immunol. 2019 Jul;41(7):e12625. (PMID: 30883834)
Exp Parasitol. 2016 Dec;171:57-66. (PMID: 27751769)
J Immunol Methods. 2005 Oct 20;305(1):84-93. (PMID: 16125721)
Proc Natl Acad Sci U S A. 1987 Apr;84(7):1814-8. (PMID: 3104905)
PLoS Negl Trop Dis. 2015 Sep 24;9(9):e0004069. (PMID: 26401956)
Prog Nucleic Acid Res Mol Biol. 1998;60:47-78. (PMID: 9594571)
Biochim Biophys Acta. 1997 Apr 10;1351(3):256-60. (PMID: 9130588)
Immunobiology. 2006;211(6-8):619-25. (PMID: 16920500)
Biomed Res Int. 2013;2013:162158. (PMID: 23509684)
Exp Parasitol. 2010 Aug;125(4):363-70. (PMID: 20214897)
PLoS Pathog. 2012 Feb;8(2):e1002534. (PMID: 22359506)
Exp Parasitol. 2011 Apr;127(4):804-10. (PMID: 21276445)
Parasitology. 2000;120 Suppl:S43-61. (PMID: 10874709)
Immunology. 2006 Apr;117(4):433-42. (PMID: 16556256)
Front Immunol. 2018 Jul 16;9:1627. (PMID: 30061894)
PLoS One. 2016 Jul 28;11(7):e0159796. (PMID: 27467391)
Parasite Immunol. 2007 Jun;29(6):319-26. (PMID: 17518950)
PLoS Negl Trop Dis. 2015 Jan 08;9(1):e3414. (PMID: 25569210)
J Infect Dis. 2013 Jan 1;207(1):186-95. (PMID: 23087431)
Oncotarget. 2016 Jun 14;7(24):35670-35679. (PMID: 27229536)
BMC Microbiol. 2015 May 19;15:104. (PMID: 25986694)
فهرسة مساهمة: Keywords: Haemonchus contortus; elongation factor-1 alpha; goat; peripheral blood mononuclear cells
سلسلة جزيئية: GENBANK HCOI_00777800
المشرفين على المادة: 0 (Helminth Proteins)
0 (Interleukin-17)
0 (Peptide Elongation Factor 1)
31C4KY9ESH (Nitric Oxide)
تواريخ الأحداث: Date Created: 20200212 Date Completed: 20200831 Latest Revision: 20200831
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC7187238
DOI: 10.1111/pim.12703
PMID: 32043596
قاعدة البيانات: MEDLINE
الوصف
تدمد:1365-3024
DOI:10.1111/pim.12703