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The zebrafish tail immobilization (ZTI) test as a new tool to assess stress-related behavior and a potential screen for drugs affecting despair-like states.

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العنوان: The zebrafish tail immobilization (ZTI) test as a new tool to assess stress-related behavior and a potential screen for drugs affecting despair-like states.
المؤلفون: Demin KA; Institute of Experimental Medicine, Almazov National Medical Research Centre, Ministry of Healthcare of Russian Federation, St. Petersburg, Russia; Institute of Translational Biomedicine, St. Petersburg State University, St. Petersburg, Russia. Electronic address: deminkasci@gmail.com., Lakstygal AM; Institute of Translational Biomedicine, St. Petersburg State University, St. Petersburg, Russia; Laboratory of Preclinical Bioscreening, Granov Russian Research Center of Radiology and Surgical Technologies, Ministry of Healthcare of Russian Federation, Pesochny, Russia., Chernysh MV; Institute of Translational Biomedicine, St. Petersburg State University, St. Petersburg, Russia., Krotova NA; Institute of Experimental Medicine, Almazov National Medical Research Centre, Ministry of Healthcare of Russian Federation, St. Petersburg, Russia; Institute of Translational Biomedicine, St. Petersburg State University, St. Petersburg, Russia., Taranov AS; Institute of Translational Biomedicine, St. Petersburg State University, St. Petersburg, Russia., Ilyin NP; Institute of Translational Biomedicine, St. Petersburg State University, St. Petersburg, Russia., Seredinskaya MV; Institute of Translational Biomedicine, St. Petersburg State University, St. Petersburg, Russia., Tagawa N; Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Tokyo, Japan., Savva AK; Laboratory of Insect Biopharmacology and Immunology, Faculty of Biology, St. Petersburg State University, St. Petersburg, Russia., Mor MS; Institute of Translational Biomedicine, St. Petersburg State University, St. Petersburg, Russia., Vasyutina ML; Institute of Experimental Medicine, Almazov National Medical Research Centre, Ministry of Healthcare of Russian Federation, St. Petersburg, Russia., Efimova EV; Institute of Translational Biomedicine, St. Petersburg State University, St. Petersburg, Russia., Kolesnikova TO; Institute of Translational Biomedicine, St. Petersburg State University, St. Petersburg, Russia., Gainetdinov RR; Institute of Translational Biomedicine, St. Petersburg State University, St. Petersburg, Russia., Strekalova T; I.M. Sechenov First Moscow State Medical University, Moscow, Russia; Maastricht University, Maastricht, The Netherlands; Research Institute of General Pathology and Pathophysiology, Moscow, Russia., Amstislavskaya TG; Scientific Research Institute of Physiology and Basic Medicine, Novosibirsk, Russia., de Abreu MS; Bioscience Institute, University of Passo Fundo, Passo Fundo, Brazil., Kalueff AV; School of Pharmacy, Southwest University, Chongqing, China; Ural Federal University, Ekaterinburg, Russia. Electronic address: avkalueff@gmail.com.
المصدر: Journal of neuroscience methods [J Neurosci Methods] 2020 May 01; Vol. 337, pp. 108637. Date of Electronic Publication: 2020 Feb 17.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 7905558 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-678X (Electronic) Linking ISSN: 01650270 NLM ISO Abbreviation: J Neurosci Methods Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam, Elsevier/North-Holland Biomedical Press.
مواضيع طبية MeSH: Pharmaceutical Preparations* , Zebrafish*, Animals ; Anxiety/drug therapy ; Behavior, Animal ; Disease Models, Animal ; Motor Activity
مستخلص: Background: Affective disorders, especially depression and anxiety, are highly prevalent, debilitating mental illnesses. Animal experimental models are a valuable tool in translational affective neuroscience research. A hallmark phenotype of clinical and experimental depression, the learned helplessness, has become a key target for 'behavioral despair'-based animal models of depression. The zebrafish (Danio rerio) has recently emerged as a promising novel organism for affective disease modeling and CNS drug screening. Despite being widely used to assess stress and anxiety-like behaviors, there are presently no clear-cut despair-like models in zebrafish.
New Method: Here, we introduce a novel behavioral paradigm, the zebrafish tail immobilization (ZTI) test, as a potential tool to assess zebrafish despair-like behavior. Conceptually similar to rodent 'despair' models, the ZTI protocol involves immobilizing the caudal half of the fish body for 5 min, leaving the cranial part to move freely, suspended vertically in a small beaker with water.
Results: To validate this model, we used exposure to low-voltage electric shock, alarm pheromone, selected antidepressants (sertraline and amitriptyline) and an anxiolytic drug benzodiazepine (phenazepam), assessing the number of mobility episodes, time spent 'moving', total distance moved and other activity measures of the cranial part of the body, using video-tracking. Both electric shock and alarm pheromone decreased zebrafish activity in this test, antidepressants increased it, and phenazepam was inactive. Furthermore, a 5-min ZTI exposure increased serotonin turnover, elevating the 5-hydroxyindoleacetic acid/serotonin ratio in zebrafish brain, while electric shock prior to ZTI elevated both this and the 3,4-dihydroxyphenylacetic acid/dopamine ratios. In contrast, preexposure to antidepressants sertraline and amitriptyline lowered both ratios, compared to the ZTI test-exposed fish.
Comparison With Existingmethod(s): The ZTI test is the first despair-like experimental model in zebrafish.
Conclusions: Collectively, this study suggests the ZTI test as a potentially useful protocol to assess stress-/despair-related behaviors, potentially relevant to CNS drug screening and behavioral phenotyping of zebrafish.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Behavioral despair; Depression; Drug screening; Immobilization; Zebrafish
المشرفين على المادة: 0 (Pharmaceutical Preparations)
تواريخ الأحداث: Date Created: 20200222 Date Completed: 20210621 Latest Revision: 20210621
رمز التحديث: 20240628
DOI: 10.1016/j.jneumeth.2020.108637
PMID: 32081675
قاعدة البيانات: MEDLINE
الوصف
تدمد:1872-678X
DOI:10.1016/j.jneumeth.2020.108637