دورية أكاديمية
أعرض في EDS

Crohn's disease: failure of a proprietary fluorescent in situ hybridization assay to detect M. avium subspecies paratuberculosis in archived frozen intestine from patients with Crohn's disease.

التفاصيل البيبلوغرافية
العنوان: Crohn's disease: failure of a proprietary fluorescent in situ hybridization assay to detect M. avium subspecies paratuberculosis in archived frozen intestine from patients with Crohn's disease.
المؤلفون: Greenstein RJ; Department of Surgery, James J. Peters Veterans Affairs Medical Center Bronx, New York, USA. BGAxis@aol.com.; Laboratory of Molecular Surgical Research, James J. Peters Veterans Affairs Medical Center Bronx, New York, USA. BGAxis@aol.com., Su L; Laboratory of Molecular Surgical Research, James J. Peters Veterans Affairs Medical Center Bronx, New York, USA., Fam PS; Mental Illness Research Education and Clinical Center (MIRECC), James J. Peters, Veterans Affairs Medical Center Bronx, New York, USA., Gurland B; Colorectal Surgery, Stanford University School of Medicine, Stanford CA, USA., Endres P; Department of Pathology, Icahn School of Medicine at Mount Sinai New York, New York, USA., Brown ST; Infectious Disease Section, James J. Peters Veterans Affairs Medical Center Bronx, New York, USA.; Department of Medicine, Icahn School of Medicine at Mt. Sinai. New York, New York, USA.
المصدر: BMC research notes [BMC Res Notes] 2020 Feb 24; Vol. 13 (1), pp. 96. Date of Electronic Publication: 2020 Feb 24.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Biomed Central Country of Publication: England NLM ID: 101462768 Publication Model: Electronic Cited Medium: Internet ISSN: 1756-0500 (Electronic) Linking ISSN: 17560500 NLM ISO Abbreviation: BMC Res Notes Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Biomed Central, 2008.
مواضيع طبية MeSH: Crohn Disease/*metabolism , DNA, Bacterial/*metabolism , In Situ Hybridization, Fluorescence/*methods , Mycobacterium avium subsp. paratuberculosis/*genetics , Paratuberculosis/*metabolism , Ruminants/*metabolism, Animals ; Crohn Disease/diagnosis ; Crohn Disease/microbiology ; DNA, Bacterial/genetics ; Diagnostic Tests, Routine ; Frozen Sections ; Humans ; Intestines/microbiology ; Mycobacterium avium subsp. paratuberculosis/physiology ; Paratuberculosis/diagnosis ; Paratuberculosis/microbiology ; Ruminants/microbiology ; Sensitivity and Specificity
مستخلص: Objectives: Although controversial, there is increasing concern that Crohn's disease may be a zoonotic infectious disease consequent to a mycobacterial infection. The most plausible candidate is M. avium subspecies paratuberculosis (MAP) that is unequivocally responsible for Johne's disease in ruminants. The purpose of this study was to evaluate a proprietary (Affymetrix™ RNA view ® ) fluorescent in situ hybridization (FISH) assay for MAP RNA. Non-identifiable intestine from patients with documented Crohn's disease was assayed according to the manufacturer's instructions and with suggested modifications. Probes were custom designed for MAP and human β-actin (as the eukaryotic housekeeping gene) from published genomes.
Results: Repetitively, false positive signal was observed in our "No-Probe" negative control. Attempts were made to correct this according to the manufacturer's suggestions (by modifying wash solutions, using recommended hydrochloric acid titration and different fluorescent filters). None prevented false positive signal in the "No-Probe" control. It is concluded that when performed according to manufactures instruction and with multiple variations on the manufactures recommended suggestions to correct for false positive signal, that the Affymetrix™ RNA view ® cannot be used to detect MAP in pre-frozen resected intestine of humans with Crohn's disease.
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فهرسة مساهمة: Keywords: Crohn disease; In situ hybridization; Johne disease; Mycobacteria; Mycobacterium avium subspecies paratuberculosis
المشرفين على المادة: 0 (DNA, Bacterial)
تواريخ الأحداث: Date Created: 20200226 Date Completed: 20201123 Latest Revision: 20201123
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC7038517
DOI: 10.1186/s13104-020-04947-0
PMID: 32093770
قاعدة البيانات: MEDLINE
الوصف
تدمد:1756-0500
DOI:10.1186/s13104-020-04947-0