دورية أكاديمية
Genotyping, virulence genes and antimicrobial resistance of Campylobacter spp.isolated during two seasonal periods in Spanish poultry farms.
| العنوان: | Genotyping, virulence genes and antimicrobial resistance of Campylobacter spp.isolated during two seasonal periods in Spanish poultry farms. |
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| المؤلفون: | García-Sánchez L; Department of Biotechnology and Food Science, University of Burgos, Burgos, Spain., Melero B; Department of Biotechnology and Food Science, University of Burgos, Burgos, Spain., Diez AM; Department of Biotechnology and Food Science, University of Burgos, Burgos, Spain., Jaime I; Department of Biotechnology and Food Science, University of Burgos, Burgos, Spain., Canepa A; Department of Biotechnology and Food Science, University of Burgos, Burgos, Spain., Rovira J; Department of Biotechnology and Food Science, University of Burgos, Burgos, Spain. Electronic address: jrovira@ubu.es. |
| المصدر: | Preventive veterinary medicine [Prev Vet Med] 2020 Mar; Vol. 176, pp. 104935. Date of Electronic Publication: 2020 Feb 20. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Scientific Publishing Country of Publication: Netherlands NLM ID: 8217463 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-1716 (Electronic) Linking ISSN: 01675877 NLM ISO Abbreviation: Prev Vet Med Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Scientific Publishing Original Publication: Amsterdam, Netherlands : Elsevier, [1982- |
| مواضيع طبية MeSH: | Chickens* , Genotype*, Campylobacter Infections/*veterinary , Campylobacter coli/*drug effects , Campylobacter jejuni/*drug effects , Drug Resistance, Bacterial/*genetics , Poultry Diseases/*epidemiology, Animals ; Campylobacter Infections/epidemiology ; Campylobacter Infections/microbiology ; Campylobacter coli/genetics ; Campylobacter coli/pathogenicity ; Campylobacter jejuni/genetics ; Campylobacter jejuni/pathogenicity ; Poultry Diseases/microbiology ; Prevalence ; Seasons ; Spain/epidemiology ; Virulence/genetics |
| مستخلص: | Campylobacter spp. are the leading causes of bacterial human gastroenteritis worldwide; being poultry farms the main source of infections. In order to obtain information on prevalence and diversity of Campylobacter-infected flocks in the North of Spain, fourteen farms were studied between autumn and spring in 2014 and 2015, respectively. Moreover, virulence genes involved in pathogenicity and antimicrobial resistance were investigated. A survey about preventive hygiene practices at farms was performed to determine the risky practices that could contribute to the presence of Campylobacter in this step of the poultry food chain. Testing the presence of Campylobacter spp. showed 43 % of the farms were positive during autumn, whereas only 31 % were positive in spring. A very high prevalence within-flock was observed (43.1 % to 88.6 %) and C. jejuni was the most prevalent species in both periods. Genotyping by pulsed field gel electrophoresis (PFGE) showed a high heterogeneity among farms (309 isolates clustered into 21 pulsotypes). Virulence genes were present in all C. jejuni isolates while cdtA and cdtC were absent in C. coli. On the contrary, the latter showed higher antimicrobial resistance than C. jejuni. This study suggests that environment might be one of the main sources for Campylobacter transmission, as water supply seemed to be a clear cause of the contamination in a specific farm. However, in other farms other environmental factors contributed to the contamination, confirming the multifactorial origin of Campylobacter colonization in broilers. Therefore, biosecurity measures in farms are crucial to reduce Campylobacter contamination, which may have important implications for human and animal health. Competing Interests: Declaration of Competing Interest The authors have not had any conflict of interest in the publication of this article. (Copyright © 2020 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Antibiotic resistance; PFGE; Poultry farms; Prevalence; Spain; Virulence genes |
| تواريخ الأحداث: | Date Created: 20200229 Date Completed: 20201019 Latest Revision: 20201019 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.prevetmed.2020.104935 |
| PMID: | 32109783 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-1716 |
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| DOI: | 10.1016/j.prevetmed.2020.104935 |