دورية أكاديمية
Detection of allopurinol and oxypurinol in canine urine by HPLC/MS-MS: Focus on veterinary clinical pharmacokinetics.
| العنوان: | Detection of allopurinol and oxypurinol in canine urine by HPLC/MS-MS: Focus on veterinary clinical pharmacokinetics. |
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| المؤلفون: | Godoy ALPC; Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Federal University of Bahia (UFBA), Rua Barão de Jeremoabo, 147, Salvador, BA, 40170-115, Brazil., de Jesus C; Teaching Hospital of Veterinary Medicine, UFBA, Av. Adhemar de Barros, 500, Salvador, BA, 40170-110, Brazil., Gonçalves RS; Teaching Hospital of Veterinary Medicine, UFBA, Av. Adhemar de Barros, 500, Salvador, BA, 40170-110, Brazil., Azeredo FJ; Department of Medicines, Faculty of Pharmacy, Federal University of Bahia (UFBA), Rua Barão de Jeremoabo, 147, Salvador, BA, 40170-115, Brazil., Rocha A; Department of Clinical, Toxicological and Bromatological Analyzes, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Avenida do Café, S / N, Ribeirão Preto, SP, 14040-903, Brazil., Marques MP; Department of Clinical, Toxicological and Bromatological Analyzes, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Avenida do Café, S / N, Ribeirão Preto, SP, 14040-903, Brazil., Lanchote VL; Department of Clinical, Toxicological and Bromatological Analyzes, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Avenida do Café, S / N, Ribeirão Preto, SP, 14040-903, Brazil., Larangeira DF; Teaching Hospital of Veterinary Medicine, UFBA, Av. Adhemar de Barros, 500, Salvador, BA, 40170-110, Brazil; Department of Veterinary Anatomy, Pathology and Clinics, School of Veterinary Medicine and Zootechny, UFBA, Salvador, BA, 40170-110, Brazil., Barrouin-Melo SM; Teaching Hospital of Veterinary Medicine, UFBA, Av. Adhemar de Barros, 500, Salvador, BA, 40170-110, Brazil; Department of Veterinary Anatomy, Pathology and Clinics, School of Veterinary Medicine and Zootechny, UFBA, Salvador, BA, 40170-110, Brazil. Electronic address: barrouin@ufba.br. |
| المصدر: | Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2020 Jun 05; Vol. 185, pp. 113204. Date of Electronic Publication: 2020 Feb 25. |
| نوع المنشور: | Journal Article; Observational Study, Veterinary; Validation Study |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: England NLM ID: 8309336 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-264X (Electronic) Linking ISSN: 07317085 NLM ISO Abbreviation: J Pharm Biomed Anal Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2006->: London : Elsevier Science Original Publication: Oxford ; New York : Pergamon Press, c1983- |
| مواضيع طبية MeSH: | Allopurinol/*urine , Dogs/*urine , Drug Monitoring/*veterinary , Leishmaniasis/*veterinary , Oxypurinol/*urine, Administration, Oral ; Allopurinol/administration & dosage ; Allopurinol/pharmacokinetics ; Animals ; Chromatography, High Pressure Liquid/methods ; Dogs/parasitology ; Drug Monitoring/methods ; Leishmania infantum/isolation & purification ; Leishmaniasis/drug therapy ; Leishmaniasis/parasitology ; Limit of Detection ; Male ; Oxypurinol/pharmacokinetics ; Reproducibility of Results ; Tandem Mass Spectrometry/methods |
| مستخلص: | Allopurinol is the most commonly used drug for the treatment of hyperuricemia in people, and in view of the risks of fatal hypersensitivity in patients with renal dysfunction, doses based on the glomerular filtration rate are proposed. In veterinary medicine, allopurinol is used in the treatment of canine leishmaniasis (CanL) caused by Leishmania infantum owing to the drug action of inhibiting the parasite's RNA synthesis. However, renal dysfunction frequently ensues from disease progression in dogs. The purpose of the present study was to standardize and validate a sensitive high-performance liquid chromatography-mass spectrometric (HPLC-MS/MS) method to determine the concentration of allopurinol and its active metabolite oxypurinol in canine urine for clinical pharmacokinetic investigation. Urine samples of eleven (11) dogs with naturally occurring CanL and in the maintenance phase of the treatment with alopurinol were used. For the chromatographic analysis of urine, the mobile phase consisted of a solution of 0.1 % formic acid (88 %) in 10 mM ammonium acetate. Separation of allopurinol and oxypurinol occurred in a flow of 0.8 mL/min on a C8 reverse phase column 5 μm, and acyclovir was the internal standard. The HPLC-MS/MS method was validated by reaching the limits of detection and quantification, reproducibility and linearity. The lower limit of quantification achieved by the method was 10 μg/mL for both allopurinol and oxypurinol. Calibration curves were prepared in blank urine added with allopurinol at concentrations of 10-1000 μg/mL, and oxypurinol at 10-200 μg/mL. Coefficients of variation of less than 15 % between intracurrent and intercurrent accuracy values were observed for both allopurinol and oxypurinol. Urine test samples remained stable after being subjected to freeze-thaw cycles and remaining at room temperature for 4 h. The method proved to be adequate to quantify allopurinol and oxypurinol in urine samples from dogs under treatment. (Copyright © 2020 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Canine model; Leishmaniasis; Translational medicine; Urinary analysis; Validation |
| المشرفين على المادة: | 63CZ7GJN5I (Allopurinol) G97OZE5068 (Oxypurinol) |
| تواريخ الأحداث: | Date Created: 20200304 Date Completed: 20210301 Latest Revision: 20210301 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.jpba.2020.113204 |
| PMID: | 32126445 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-264X |
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| DOI: | 10.1016/j.jpba.2020.113204 |