دورية أكاديمية
أعرض في EDS

Resistance to Chilo infuscatellus (Lepidoptera: Pyraloidea) in transgenic lines of sugarcane expressing Bacillus thuringiensis derived Vip3A protein.

التفاصيل البيبلوغرافية
العنوان: Resistance to Chilo infuscatellus (Lepidoptera: Pyraloidea) in transgenic lines of sugarcane expressing Bacillus thuringiensis derived Vip3A protein.
المؤلفون: Riaz S; Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, 53700, Pakistan., Nasir IA; Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, 53700, Pakistan., Bhatti MU; Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, 53700, Pakistan., Adeyinka OS; Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, 53700, Pakistan., Toufiq N; Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, 53700, Pakistan., Yousaf I; Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, 53700, Pakistan., Tabassum B; Centre of Excellence in Molecular Biology, University of the Punjab, Lahore, 53700, Pakistan. bushra.cemb@gmail.com.
المصدر: Molecular biology reports [Mol Biol Rep] 2020 Apr; Vol. 47 (4), pp. 2649-2658. Date of Electronic Publication: 2020 Mar 03.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Reidel Country of Publication: Netherlands NLM ID: 0403234 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-4978 (Electronic) Linking ISSN: 03014851 NLM ISO Abbreviation: Mol Biol Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Dordrecht, Boston, Reidel.
مواضيع طبية MeSH: Bacterial Proteins/*metabolism , Biological Control Agents/*metabolism , Saccharum/*genetics, Animals ; Bacillus thuringiensis/genetics ; Bacterial Proteins/genetics ; Biological Control Agents/pharmacology ; Insecta/genetics ; Insecticides ; Larva/metabolism ; Lepidoptera/genetics ; Moths/genetics ; Moths/pathogenicity ; Plants, Genetically Modified/genetics
مستخلص: Sustainable agriculture requires management of insect pests through resistance development. The biological potential of Cry toxins and Vip protein, derived from Bacillus species, is widely recognized in this context. The identification, evaluation of new insecticidal protein genes with different mode of action and entomotoxicity against sugarcane stem borer (Chilo infuscatellus) is important to overcome evolved insect resistance. In this study, we reported the generation of transgenic sugarcane lines expressing Vip3A toxin driven by polyubiquitin promoter for resistance against sugarcane stem borer. The V 0 transgenic sugarcane plants were initially characterized by GUS histochemical staining, PCR and Southern blot assays that confirmed genetic transformation of twelve independent sugarcane lines. Variable transgene expression was found among transgenic sugarcane lines when revealed through Realtime quantitative PCR (RT-qPCR) with highest in S10 line while minimum was observed in V5 line. A similar expression pattern was observed in transgenic sugarcane lines for Vip3A protein concentration which ranged from 5.35 to 8.89 µg/mL. A direct correlation was observed between the Vip3A protein and Vip3A transgene expression in the transgenic sugarcane lines. In in-vitro insect bioassay on V 1 , Vip3A transgenic sugarcane lines exhibited high resistance to C. infuscatellus with upto 100% mortality compared to the control sugarcane line. Our findings suggest that a single copy insertion of Vip3A gene in transgenic sugarcane lines render them resistant to borer and these lines can be potentially used for generation of insect resistant transgenic sugarcane and could also be employed in gene pyramiding with Bt toxin to prolong resistance.
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فهرسة مساهمة: Keywords: Bacillus thuringenesis; Insect bioassay; Sugarcane stem borer; Transgenic; Vip3A protein
المشرفين على المادة: 0 (Bacterial Proteins)
0 (Biological Control Agents)
0 (Insecticides)
0 (Vip3A protein, Bacillus thuringiensis)
تواريخ الأحداث: Date Created: 20200305 Date Completed: 20210201 Latest Revision: 20210201
رمز التحديث: 20231215
DOI: 10.1007/s11033-020-05355-0
PMID: 32128710
قاعدة البيانات: MEDLINE
الوصف
تدمد:1573-4978
DOI:10.1007/s11033-020-05355-0