دورية أكاديمية
Spectral and photochemical diversity of tandem cysteine cyanobacterial phytochromes.
| العنوان: | Spectral and photochemical diversity of tandem cysteine cyanobacterial phytochromes. |
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| المؤلفون: | Song JY; Department of Biological Sciences, Chungnam National University, Daejeon 34134, Korea., Lee HY; Department of Biological Sciences, Chungnam National University, Daejeon 34134, Korea., Yang HW; Department of Biological Sciences, Chungnam National University, Daejeon 34134, Korea., Song JJ; Department of Biological Science and KI for the BioCentury, Korea Advanced Institute of Science and Technology, Daejeon 34141, Korea., Lagarias JC; Department of Molecular and Cellular Biology, University of California Davis, Davis, California 95616 jclagarias@ucdavis.edu., Park YI; Department of Biological Sciences, Chungnam National University, Daejeon 34134, Korea yipark@cnu.ac.kr. |
| المصدر: | The Journal of biological chemistry [J Biol Chem] 2020 May 08; Vol. 295 (19), pp. 6754-6766. Date of Electronic Publication: 2020 Mar 17. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 2985121R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1083-351X (Electronic) Linking ISSN: 00219258 NLM ISO Abbreviation: J Biol Chem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [New York, NY] : Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Original Publication: Baltimore, MD : American Society for Biochemistry and Molecular Biology |
| مواضيع طبية MeSH: | Bacterial Proteins/*chemistry , Cyanobacteria/*chemistry , Photoreceptors, Microbial/*chemistry , Phytochrome/*chemistry, Amino Acid Substitution ; Bacterial Proteins/genetics ; Cyanobacteria/genetics ; Mutation, Missense ; Photoreceptors, Microbial/genetics ; Phytochrome/genetics |
| مستخلص: | The atypical trichromatic cyanobacterial phytochrome Np TP1 from Nostoc punctiforme ATCC 29133 is a linear tetrapyrrole (bilin)-binding photoreceptor protein that possesses tandem-cysteine residues responsible for shifting its light-sensing maximum to the violet spectral region. Using bioinformatics and phylogenetic analyses, here we established that tandem-cysteine cyanobacterial phytochromes (TCCPs) compose a well-supported monophyletic phytochrome lineage distinct from prototypical red/far-red cyanobacterial phytochromes. To investigate the light-sensing diversity of this family, we compared the spectroscopic properties of Np TP1 (here renamed Np TCCP) with those of three phylogenetically diverged TCCPs identified in the draft genomes of Tolypothrix sp. PCC7910, Scytonema sp. PCC10023, and Gloeocapsa sp. PCC7513. Recombinant photosensory core modules of To TCCP, Sc TCCP, and Gl TCCP exhibited violet-blue-absorbing dark-states consistent with dual thioether-linked phycocyanobilin (PCB) chromophores. Photoexcitation generated singly-linked photoproduct mixtures with variable ratios of yellow-orange and red-absorbing species. The photoproduct ratio was strongly influenced by pH and by mutagenesis of TCCP- and phytochrome-specific signature residues. Our experiments support the conclusion that both photoproduct species possess protonated 15 E bilin chromophores, but differ in the ionization state of the noncanonical "second" cysteine sulfhydryl group. We found that the ionization state of this and other residues influences subsequent conformational change and downstream signal transmission. We also show that tandem-cysteine phytochromes present in eukaryotes possess similar amino acid substitutions within their chromophore-binding pocket, which tune their spectral properties in an analogous fashion. Taken together, our findings provide a roadmap for tailoring the wavelength specificity of plant phytochromes to optimize plant performance in diverse natural and artificial light environments. (© 2020 Song et al.) |
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| معلومات مُعتمدة: | R01 GM068552 United States GM NIGMS NIH HHS |
| فهرسة مساهمة: | Keywords: Color sensing; algae; cyanobacteria; dual cysteine phytochromes; photoreceptor; photosynthesis; phycocyanobilin; plant; protochromism; violet and blue light photoreceptors |
| سلسلة جزيئية: | PDB 2VEA; 2O9B |
| المشرفين على المادة: | 0 (Bacterial Proteins) 0 (Photoreceptors, Microbial) 11121-56-5 (Phytochrome) |
| تواريخ الأحداث: | Date Created: 20200319 Date Completed: 20201223 Latest Revision: 20210508 |
| رمز التحديث: | 20240829 |
| مُعرف محوري في PubMed: | PMC7212627 |
| DOI: | 10.1074/jbc.RA120.012950 |
| PMID: | 32184354 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1083-351X |
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| DOI: | 10.1074/jbc.RA120.012950 |