دورية أكاديمية
أعرض في EDS

Extended hypothermic oxygenated machine perfusion enables ex situ preservation of porcine livers for up to 24 hours.

التفاصيل البيبلوغرافية
العنوان: Extended hypothermic oxygenated machine perfusion enables ex situ preservation of porcine livers for up to 24 hours.
المؤلفون: Brüggenwirth IMA; Department of Surgery, Section of Hepatobiliary Surgery and Liver Transplantation, University Medical Center Groningen, Groningen, The Netherlands., van Leeuwen OB; Department of Surgery, Section of Hepatobiliary Surgery and Liver Transplantation, University Medical Center Groningen, Groningen, The Netherlands., de Vries Y; Department of Surgery, Section of Hepatobiliary Surgery and Liver Transplantation, University Medical Center Groningen, Groningen, The Netherlands., Bodewes SB; Department of Surgery, Section of Hepatobiliary Surgery and Liver Transplantation, University Medical Center Groningen, Groningen, The Netherlands., Adelmeijer J; Surgical Research Laboratory, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands., Wiersema-Buist J; Surgical Research Laboratory, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands., Lisman T; Surgical Research Laboratory, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands., Martins PN; Division of Organ Transplantation, Department of Surgery, UMass Memorial Medical Center, University of Massachusetts, Worcester, MA, United States., de Meijer VE; Department of Surgery, Section of Hepatobiliary Surgery and Liver Transplantation, University Medical Center Groningen, Groningen, The Netherlands., Porte RJ; Department of Surgery, Section of Hepatobiliary Surgery and Liver Transplantation, University Medical Center Groningen, Groningen, The Netherlands.
المصدر: JHEP reports : innovation in hepatology [JHEP Rep] 2020 Feb 17; Vol. 2 (2), pp. 100092. Date of Electronic Publication: 2020 Feb 17 (Print Publication: 2020).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier B.V Country of Publication: Netherlands NLM ID: 101761237 Publication Model: eCollection Cited Medium: Internet ISSN: 2589-5559 (Electronic) Linking ISSN: 25895559 NLM ISO Abbreviation: JHEP Rep Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: [Amsterdam] : Elsevier B.V., [2019]-
مستخلص: Background & Aims: End-ischemic hypothermic oxygenated machine perfusion (HOPE) of the donor liver for 1-2 h mitigates ischemia-reperfusion injury during subsequent liver transplantation. Extended preservation time may be preferred to facilitate difficult recipient hepatectomy or to optimize logistics. We therefore investigated whether end-ischemic dual HOPE (DHOPE) could extend preservation time for up to 24 h using a porcine liver reperfusion model.
Methods: Following 30 min warm ischemia, porcine livers were subjected to 2 h static cold storage (SCS), followed by 2 h, 6 h, or 24 h DHOPE (n = 6 per group). Subsequent normothermic reperfusion was performed for 4 h using autologous blood. Two livers preserved by 24 h SCS served as additional controls. A proof of principle confirmation was carried out in 2 discarded human livers subjected to extended DHOPE. Hepatocellular and cholangiocyte injury and function were assessed. Oxidative stress levels and histology were compared between groups.
Results: Perfusion flows remained stable during DHOPE, regardless of duration. After normothermic reperfusion, livers perfused for 24 h by DHOPE had similar lactate clearance, blood pH, glucose, and alanine aminotransferase levels, and biliary pH, bicarbonate, and LDH levels, as livers perfused for 2 h and 6 h. Levels of malondialdehyde and high-mobility group box 1 in serum and liver parenchyma were similar for all groups. Histological analysis of bile ducts and liver parenchyma revealed no differences between the groups. Extended DHOPE in discarded human livers preserved hepatocellular and cholangiocyte function and histology after reperfusion. In contrast, livers preserved by 24 h SCS were non-functioning.
Conclusion: Extended end-ischemic DHOPE enabled successful preservation of porcine and discarded human donor livers for up to 24 h. Extended DHOPE enables safe extension of preservation time, which may facilitate allocation and transplantation from a logistical perspective, and further expand the donor pool.
Lay Summary: It has been suggested that preserving liver grafts with a technique called (dual) hypothermic oxygenated machine perfusion ([D]HOPE) leads to better outcomes after transplantation than if livers are stored on ice, especially if an organ is of lesser quality. In this study, we showed that DHOPE could be used to preserve liver grafts for up to 24 h. This extended procedure could be used globally to facilitate transplantation and expand the donor pool.
Competing Interests: The authors declare no conflicts of interest that pertain to this work. Please refer to the accompanying ICMJE disclosure forms for further details.
(© 2020 The Author(s).)
References: Ann Surg. 2017 Jan;265(1):e1-e2. (PMID: 27295096)
J Hepatol. 2019 Jan;70(1):203-205. (PMID: 30409464)
J Hepatol. 2014 Jun;60(6):1172-9. (PMID: 24560661)
Ann Surg. 2015 Nov;262(5):764-70; discussion 770-1. (PMID: 26583664)
Liver Transpl. 2018 May;24(5):655-664. (PMID: 29369470)
J Hepatol. 2019 Jan;70(1):50-57. (PMID: 30342115)
Transpl Int. 2019 Feb;32(2):218-224. (PMID: 30251360)
Transplantation. 1987 Apr;43(4):468-71. (PMID: 3554638)
Liver Transpl. 2015 Oct;21(10):1300-11. (PMID: 26097213)
Transplantation. 2016 Apr;100(4):825-35. (PMID: 26863473)
Transplantation. 1990 Dec;50(6):940-3. (PMID: 2256166)
Cryobiology. 2004 Jun;48(3):322-32. (PMID: 15157780)
Transpl Int. 2015 Jun;28(6):677-89. (PMID: 24852621)
Transplantation. 2004 Jun 15;77(11):1676-82. (PMID: 15201666)
PLoS One. 2014 Nov 04;9(11):e110642. (PMID: 25369327)
BMC Gastroenterol. 2019 Mar 12;19(1):40. (PMID: 30866837)
PLoS One. 2017 Nov 27;12(11):e0188494. (PMID: 29176869)
Transpl Int. 2010 Sep;23(9):944-50. (PMID: 20210932)
Am J Transplant. 2013 Jun;13(6):1450-60. (PMID: 23617781)
J Hepatol. 2014 Sep;61(3):672-84. (PMID: 24798616)
Ann Surg. 2019 Nov;270(5):906-914. (PMID: 31633615)
Am J Transplant. 2019 Apr;19(4):1202-1211. (PMID: 30588774)
Liver Transpl. 2018 Apr;24(4):557-561. (PMID: 29316185)
Am J Transplant. 2010 Aug;10(8):1842-9. (PMID: 20659090)
IEEE Trans Biomed Eng. 2011 Jan;58(1):25-35. (PMID: 20709637)
Transplantation. 2019 Jul;103(7):1405-1413. (PMID: 30395120)
Curr Transplant Rep. 2018;5(1):93-102. (PMID: 29564206)
Liver Transpl. 2018 Dec;24(12):1716-1725. (PMID: 30168653)
Transplantation. 2017 Jul;101(7):e223-e224. (PMID: 28353493)
Liver Transpl. 2017 Feb;23(2):207-220. (PMID: 27809409)
Am J Transplant. 2006 Jun;6(6):1355-66. (PMID: 16686759)
Am J Transplant. 2013 May;13(5):1327-35. (PMID: 23463950)
Transplantation. 1990 Feb;49(2):258-60. (PMID: 2305453)
Br J Surg. 2017 Jun;104(7):907-917. (PMID: 28394402)
Ann Biomed Eng. 2006 Dec;34(12):1924-34. (PMID: 17066322)
Am J Transplant. 2016 Mar;16(3):794-807. (PMID: 26663737)
Transpl Int. 1992;5 Suppl 1:S403-7. (PMID: 14621832)
Transplantation. 1995 Sep 15;60(5):462-6. (PMID: 7676494)
PLoS One. 2014 Feb 14;9(2):e88521. (PMID: 24551114)
J Hepatol. 2013 Feb;58(2):278-86. (PMID: 23063573)
فهرسة مساهمة: Keywords: 8-OHdG, 8-hydroxydeoxyguanosine; ALT, alanine aminotransferase; DCD, donation after circulatory death; DHOPE, dual hypothermic oxygenated machine perfusion; ECD, extended criteria donor; HMGB-1, high-mobility group box 1; HMP, hypothermic machine perfusion; HOPE, hypothermic oxygenated machine perfusion; HPF, high-powered field; IL-6, interleukin 6; LDH, lactate dehydrogenase; MDA, malondialdehyde; NMP, normothermic machine perfusion; SCS, static cold storage; SEM, standard error of the mean; TNFα, tumor necrosis factor-alpha; UW, University of Wisconsin; VWF, von Willebrand factor; cfDNA, cell-free DNA; donation after circulatory death; extended preservation; hypothermic machine perfusion; liver preservation; sTM, soluble thrombomodulin
تواريخ الأحداث: Date Created: 20200321 Latest Revision: 20220413
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC7078381
DOI: 10.1016/j.jhepr.2020.100092
PMID: 32195456
قاعدة البيانات: MEDLINE
الوصف
تدمد:2589-5559
DOI:10.1016/j.jhepr.2020.100092