دورية أكاديمية
أعرض في EDS

Targeted cell imaging properties of a deep red luminescent iridium(iii) complex conjugated with a c-Myc signal peptide.

التفاصيل البيبلوغرافية
العنوان: Targeted cell imaging properties of a deep red luminescent iridium(iii) complex conjugated with a c-Myc signal peptide.
المؤلفون: Day AH; School of Chemistry , Cardiff University , Main Building , Cardiff , CF10 3AT , UK . Email: popesj@cardiff.ac.uk., Übler MH; School of Chemistry , Cardiff University , Main Building , Cardiff , CF10 3AT , UK . Email: popesj@cardiff.ac.uk., Best HL; School of Biosciences , Cardiff University , Sir Martin Evans Building , Cardiff , UK., Lloyd-Evans E; School of Biosciences , Cardiff University , Sir Martin Evans Building , Cardiff , UK., Mart RJ; School of Chemistry , Cardiff University , Main Building , Cardiff , CF10 3AT , UK . Email: popesj@cardiff.ac.uk., Fallis IA; School of Chemistry , Cardiff University , Main Building , Cardiff , CF10 3AT , UK . Email: popesj@cardiff.ac.uk., Allemann RK; School of Chemistry , Cardiff University , Main Building , Cardiff , CF10 3AT , UK . Email: popesj@cardiff.ac.uk., Al-Wattar EAH; School of Chemistry , Cardiff University , Main Building , Cardiff , CF10 3AT , UK . Email: popesj@cardiff.ac.uk., Keymer NI; School of Chemistry , Cardiff University , Main Building , Cardiff , CF10 3AT , UK . Email: popesj@cardiff.ac.uk., Buurma NJ; School of Chemistry , Cardiff University , Main Building , Cardiff , CF10 3AT , UK . Email: popesj@cardiff.ac.uk., Pope SJA; School of Chemistry , Cardiff University , Main Building , Cardiff , CF10 3AT , UK . Email: popesj@cardiff.ac.uk.
المصدر: Chemical science [Chem Sci] 2020 Jan 08; Vol. 11 (6), pp. 1599-1606. Date of Electronic Publication: 2020 Jan 08 (Print Publication: 2020).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 101545951 Publication Model: eCollection Cited Medium: Print ISSN: 2041-6520 (Print) Linking ISSN: 20416520 NLM ISO Abbreviation: Chem Sci Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Cambridge, UK : Royal Society of Chemistry, [2010]-
مستخلص: A nuclear localisation sequence (NLS) peptide, PAAKRVKLD, derived from the human c-Myc regulator gene, has been functionalised with a long wavelength ( λ ex = 550 nm; λ em = 677 nm) cyclometalated organometallic iridium(iii) complex to give the conjugate Ir-CMYC . Confocal fluorescence microscopy studies on human fibroblast cells imaged after 18-24 h incubation show that Ir-CMYC concentrations of 80-100 μM promote good cell uptake and nuclear localisation, which was confirmed though co-localisation studies using Hoechst 33342. In comparison, a structurally related, photophysically analogous iridium(iii) complex lacking the peptide sequence, Ir-PYR , showed very different biological behaviour, with no evidence of nuclear, lysosomal or autophagic vesicle localisation and significantly increased toxicity to the cells at concentrations >10 μM that induced mitochondrial dysfunction. Supporting UV-visible and circular dichroism spectroscopic studies show that Ir-PYR and Ir-CMYC display similarly low affinities for DNA ( ca. 10 3 M -1 ), consistent with electrostatic binding. Therefore the translocation and nuclear uptake properties of Ir-CMYC are attributed to the presence of the PAAKRVKLD nuclear localisation sequence in this complex.
(This journal is © The Royal Society of Chemistry 2020.)
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تواريخ الأحداث: Date Created: 20200325 Latest Revision: 20231103
رمز التحديث: 20231103
مُعرف محوري في PubMed: PMC7069228
DOI: 10.1039/c9sc05568a
PMID: 32206278
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-6520
DOI:10.1039/c9sc05568a