دورية أكاديمية
أعرض في EDS

Development of a genetic toolset for the highly engineerable and metabolically versatile Acinetobacter baylyi ADP1.

التفاصيل البيبلوغرافية
العنوان: Development of a genetic toolset for the highly engineerable and metabolically versatile Acinetobacter baylyi ADP1.
المؤلفون: Biggs BW; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL 60208, USA.; Biotechnology Training Program, Northwestern University, Evanston, IL 60208, USA., Bedore SR; Department of Microbiology, University of Georgia, Athens, GA 30602, USA., Arvay E; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL 60208, USA.; Biotechnology Training Program, Northwestern University, Evanston, IL 60208, USA., Huang S; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL 60208, USA., Subramanian H; Master of Science in Biotechnology Program, Northwestern University, Evanston, IL 60208, USA., McIntyre EA; Department of Microbiology, University of Georgia, Athens, GA 30602, USA., Duscent-Maitland CV; Department of Microbiology, University of Georgia, Athens, GA 30602, USA., Neidle EL; Department of Microbiology, University of Georgia, Athens, GA 30602, USA., Tyo KEJ; Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL 60208, USA.
المصدر: Nucleic acids research [Nucleic Acids Res] 2020 May 21; Vol. 48 (9), pp. 5169-5182.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE
أسماء مطبوعة: Publication: 1992- : Oxford : Oxford University Press
Original Publication: London, Information Retrieval ltd.
مواضيع طبية MeSH: Metabolic Engineering*, Acinetobacter/*genetics, Acinetobacter/metabolism ; Cloning, Molecular/methods ; Genome, Bacterial ; Genomics ; Lignin/metabolism ; Promoter Regions, Genetic ; Ribosomes/metabolism
مستخلص: One primary objective of synthetic biology is to improve the sustainability of chemical manufacturing. Naturally occurring biological systems can utilize a variety of carbon sources, including waste streams that pose challenges to traditional chemical processing, such as lignin biomass, providing opportunity for remediation and valorization of these materials. Success, however, depends on identifying micro-organisms that are both metabolically versatile and engineerable. Identifying organisms with this combination of traits has been a historic hindrance. Here, we leverage the facile genetics of the metabolically versatile bacterium Acinetobacter baylyi ADP1 to create easy and rapid molecular cloning workflows, including a Cas9-based single-step marker-less and scar-less genomic integration method. In addition, we create a promoter library, ribosomal binding site (RBS) variants and test an unprecedented number of rationally integrated bacterial chromosomal protein expression sites and variants. At last, we demonstrate the utility of these tools by examining ADP1's catabolic repression regulation, creating a strain with improved potential for lignin bioprocessing. Taken together, this work highlights ADP1 as an ideal host for a variety of sustainability and synthetic biology applications.
(© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.)
References: Annu Rev Microbiol. 1996;50:553-90. (PMID: 8905091)
Metabolomics. 2014;10(6):1223-1238. (PMID: 25374488)
Curr Opin Biotechnol. 2016 Dec;42:40-53. (PMID: 26974563)
Biotechnol Biofuels. 2019 Apr 23;12:91. (PMID: 31044004)
J Bacteriol. 1996 Dec;178(23):6833-41. (PMID: 8955304)
ACS Synth Biol. 2018 Feb 16;7(2):727-738. (PMID: 29366319)
ACS Synth Biol. 2016 Sep 16;5(9):942-7. (PMID: 27197833)
Biotechnol Bioeng. 2006 May 5;94(1):151-8. (PMID: 16496398)
Microbiology. 2010 May;156(Pt 5):1313-1322. (PMID: 20110298)
Metab Eng. 2015 May;29:86-96. (PMID: 25769287)
Nat Chem. 2019 Mar;11(3):190-195. (PMID: 30792512)
J Ind Microbiol Biotechnol. 2018 Jul;45(7):517-527. (PMID: 29299733)
PLoS One. 2010 Oct 08;5(10):e13244. (PMID: 20949038)
Curr Opin Microbiol. 2009 Oct;12(5):568-76. (PMID: 19709925)
Front Microbiol. 2016 May 11;7:694. (PMID: 27242719)
Nat Biotechnol. 2009 Aug;27(8):760-5. (PMID: 19633654)
Metab Eng Commun. 2017 Apr 15;5:1-8. (PMID: 29188179)
Cell Syst. 2019 Mar 27;8(3):212-225.e9. (PMID: 30904377)
Nat Methods. 2016 Oct;13(10):849-51. (PMID: 27571549)
IUBMB Life. 2011 Dec;63(12):1075-80. (PMID: 22034222)
Proc Natl Acad Sci U S A. 2002 May 28;99(11):7693-8. (PMID: 12032345)
ACS Synth Biol. 2019 Mar 15;8(3):521-531. (PMID: 30703321)
Annu Rev Microbiol. 2005;59:519-51. (PMID: 16153178)
Microb Cell Fact. 2018 Feb 08;17(1):19. (PMID: 29422050)
Proc Natl Acad Sci U S A. 2018 Jul 3;115(27):7105-7110. (PMID: 29915086)
Chem Soc Rev. 2018 Feb 5;47(3):852-908. (PMID: 29318245)
Appl Environ Microbiol. 2012 Jan;78(1):280-3. (PMID: 22020504)
J Bacteriol. 1969 Apr;98(1):281-8. (PMID: 5781579)
Appl Environ Microbiol. 2003 Sep;69(9):5627-35. (PMID: 12957953)
ACS Synth Biol. 2018 Jan 19;7(1):287-291. (PMID: 29061047)
Mol Syst Biol. 2008;4:174. (PMID: 18319726)
Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6640-5. (PMID: 10829079)
Microb Cell Fact. 2019 Mar 11;18(1):48. (PMID: 30857542)
Appl Environ Microbiol. 2017 Aug 17;83(17):. (PMID: 28667117)
Nat Methods. 2013 Jul;10(7):659-64. (PMID: 23727987)
Microb Cell Fact. 2011 May 18;10:36. (PMID: 21592360)
Proc Natl Acad Sci U S A. 2014 Aug 19;111(33):12013-8. (PMID: 25092344)
ACS Chem Biol. 2008 Jan 18;3(1):64-76. (PMID: 18205292)
Nucleic Acids Res. 2014 Oct;42(18):11383-92. (PMID: 25209233)
Appl Environ Microbiol. 2003 Mar;69(3):1598-606. (PMID: 12620848)
Curr Opin Chem Biol. 2016 Oct;34:20-29. (PMID: 27239751)
Proc Natl Acad Sci U S A. 2010 Jul 20;107(29):13087-92. (PMID: 20616070)
Appl Environ Microbiol. 2006 Jan;72(1):932-6. (PMID: 16391138)
Nat Methods. 2013 Apr;10(4):354-60. (PMID: 23474465)
Acta Crystallogr D Biol Crystallogr. 2013 Oct;69(Pt 10):1995-2007. (PMID: 24100318)
Curr Opin Biotechnol. 2014 Oct;29:156-62. (PMID: 24927371)
Nucleic Acids Res. 2004 Oct 28;32(19):5780-90. (PMID: 15514111)
Mol Microbiol. 2012 Feb;83(3):520-35. (PMID: 22211470)
Nat Commun. 2019 May 9;10(1):2040. (PMID: 31068573)
J Bacteriol. 1989 Oct;171(10):5410-21. (PMID: 2793826)
PLoS One. 2015 Sep 08;10(9):e0137466. (PMID: 26348330)
Chem Soc Rev. 2012 Feb 21;41(4):1437-51. (PMID: 22033698)
معلومات مُعتمدة: T32 GM008449 United States GM NIGMS NIH HHS
المشرفين على المادة: 9005-53-2 (Lignin)
SCR Organism: Acinetobacter baylyi
تواريخ الأحداث: Date Created: 20200405 Date Completed: 20200908 Latest Revision: 20200908
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC7229861
DOI: 10.1093/nar/gkaa167
PMID: 32246719
قاعدة البيانات: MEDLINE
الوصف
تدمد:1362-4962
DOI:10.1093/nar/gkaa167