دورية أكاديمية
أعرض في EDS

Rapid structural characterisation of benzylisoquinoline and aporphine alkaloids from Ocotea spixiana acaricide extract by HPTLC-DESI-MS n .

التفاصيل البيبلوغرافية
العنوان: Rapid structural characterisation of benzylisoquinoline and aporphine alkaloids from Ocotea spixiana acaricide extract by HPTLC-DESI-MS n .
المؤلفون: Conceição RS; Department of Health, State University of Feira de Santana, Feira de Santana, Bahia, Brazil.; Centre for Research in Mass Spectrometry, Department of Chemistry, York University, Toronto, Ontario, Canada., Reis IMA; Department of Health, State University of Feira de Santana, Feira de Santana, Bahia, Brazil., Cerqueira APM; Department of Health, State University of Feira de Santana, Feira de Santana, Bahia, Brazil., Perez CJ; Centre for Research in Mass Spectrometry, Department of Chemistry, York University, Toronto, Ontario, Canada., Junior MCDS; Department of Health, State University of Feira de Santana, Feira de Santana, Bahia, Brazil., Branco A; Department of Health, State University of Feira de Santana, Feira de Santana, Bahia, Brazil., Ifa DR; Centre for Research in Mass Spectrometry, Department of Chemistry, York University, Toronto, Ontario, Canada., Botura MB; Department of Health, State University of Feira de Santana, Feira de Santana, Bahia, Brazil.
المصدر: Phytochemical analysis : PCA [Phytochem Anal] 2020 Nov; Vol. 31 (6), pp. 711-721. Date of Electronic Publication: 2020 Apr 15.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Wiley Country of Publication: England NLM ID: 9200492 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1099-1565 (Electronic) Linking ISSN: 09580344 NLM ISO Abbreviation: Phytochem Anal Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Chichester, Sussex, UK : Wiley, c1990-
مواضيع طبية MeSH: Acaricides*/pharmacology , Alkaloids*/pharmacology , Aporphines*/pharmacology , Benzylisoquinolines* , Ocotea*, Plant Extracts/pharmacology ; Tandem Mass Spectrometry
مستخلص: Introduction: Lauraceae alkaloids are a structurally diverse class of plant specialised secondary metabolites that play an important role in modern pharmacotherapy, being useful as well as model compounds for the development of synthetic analogues. However, alkaloids characterisation is challenging due to low concentrations, the complexity of plant extracts, and long processes for accurate structural determinations.
Objective: The use of high-performance thin layer chromatography coupled with desorption electrospray ionisation multistage mass spectrometry (HPTLC DESI-MS n ) as a fast tool to identify alkaloids present in Ocotea spixiana extract and evaluate the extract's acaricide activity.
Methods: Ocotea spixiana twigs were extracted by conventional liquid-liquid partitioning. HPTLC analysis of the ethyl acetate extract was performed to separate isobaric alkaloids prior to DESI-MS n analysis, performed from MS 3 up to MS 7 . The extract's acaricide activity against Rhipicephalus microplus was evaluated by in vitro (larval immersion test) and in silico tests.
Results: HPTLC-DESI-MS n analysis was performed to identify a total of 13 aporphine and four benzylisoquinoline-type alkaloids reported for the first time in O. spixiana. In vitro evaluation of the extract and the alkaloid boldine showed significant activity against R. microplus larvae. It was established in silico that boldine had important intermolecular interactions with R. microplus acetylcholinesterase enzyme.
Conclusion: The present study demonstrated that HPTLC-DESI-MS n is a useful analytical tool to identify isoquinoline alkaloids in plant extracts. The acaricide activity of the O. spixiana ethyl acetate extract can be correlated to the presence of alkaloids.
(© 2020 John Wiley & Sons, Ltd.)
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معلومات مُعتمدة: 409592/2018-8 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Universidade Estadual de Feira de Santana (UEFS); Fundacão de Amparo à Pesquisa do Estado da Bahia (FAPESB); 001 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES)
فهرسة مساهمة: Keywords: HPTLC-DESI-MSn; Ocotea spixiana; acaricide activity; alkaloids
المشرفين على المادة: 0 (Acaricides)
0 (Alkaloids)
0 (Aporphines)
0 (Benzylisoquinolines)
0 (Plant Extracts)
تواريخ الأحداث: Date Created: 20200416 Date Completed: 20201208 Latest Revision: 20201214
رمز التحديث: 20231215
DOI: 10.1002/pca.2935
PMID: 32291820
قاعدة البيانات: MEDLINE
الوصف
تدمد:1099-1565
DOI:10.1002/pca.2935