دورية أكاديمية
Cyclooxygenase-2 expression is associated with infiltration of inflammatory cells in oral and skin canine melanomas.
| العنوان: | Cyclooxygenase-2 expression is associated with infiltration of inflammatory cells in oral and skin canine melanomas. |
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| المؤلفون: | Silveira TL; Department of Pathology, Federal University of Minas Gerais, Belo Horizonte, Brazil., Veloso ES; Department of Pathology, Federal University of Minas Gerais, Belo Horizonte, Brazil., Gonçalves INN; Department of Pathology, Federal University of Minas Gerais, Belo Horizonte, Brazil., Costa RF; Department of Pathology, Federal University of Minas Gerais, Belo Horizonte, Brazil., Rodrigues MA; Department of Pathology, Federal University of Minas Gerais, Belo Horizonte, Brazil., Cassali GD; Department of Pathology, Federal University of Minas Gerais, Belo Horizonte, Brazil., Del Puerto HL; Department of Pathology, Federal University of Minas Gerais, Belo Horizonte, Brazil., Pang LY; The Roslin Institute, The University of Edinburgh, Edinburgh, Scotland., Argyle DJ; The Roslin Institute, The University of Edinburgh, Edinburgh, Scotland., Ferreira E; Department of Pathology, Federal University of Minas Gerais, Belo Horizonte, Brazil. |
| المصدر: | Veterinary and comparative oncology [Vet Comp Oncol] 2020 Dec; Vol. 18 (4), pp. 727-738. Date of Electronic Publication: 2020 May 03. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Blackwell Country of Publication: England NLM ID: 101185242 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-5829 (Electronic) Linking ISSN: 14765810 NLM ISO Abbreviation: Vet Comp Oncol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Oxford, UK : Blackwell, [2003]- |
| مواضيع طبية MeSH: | Cyclooxygenase 2/*metabolism , Dog Diseases/*metabolism , Melanoma/*veterinary , Mouth Neoplasms/*veterinary , Skin Neoplasms/*veterinary, Animals ; Biomarkers, Tumor/analysis ; Dog Diseases/pathology ; Dogs ; Immunohistochemistry ; Melanoma/metabolism ; Melanoma/pathology ; Mouth Neoplasms/metabolism ; Mouth Neoplasms/pathology ; Skin Neoplasms/metabolism ; Skin Neoplasms/pathology ; Melanoma, Cutaneous Malignant |
| مستخلص: | Melanoma is a fast-growing tumour in dogs and represents 7% of the total malignant neoplasms from the skin and is the most common tumour found in the oral cavity. In these tumours, high expression of cyclooxygenase-2 (COX-2) is associated with a poor prognosis. The aim of this study was to verify if the overexpression of COX-2 is related to the modulation of lymphocytes and if it is associated with the angiogenic and proliferative capacity of the melanoma. Canine melanoma samples (n = 85) were analysed by immunohistochemistry to detect the expression of S-100, Melan-A, PNL-2, COX-2, Factor VIII, Ki-67 and immune cells markers (CD3, CD4, FOXP3 and MAC387); and expression levels of MAC387, NOS and CD206 were determined by immunofluorescence. Our study showed a concurrent difference between the expression of COX-2 and inflammatory cell infiltration: Oral melanomas showed positivity for COX-2 in 34% of the cases and this expression was associated with CD3 positivity in the inflammatory infiltrate and angiogenesis; whereas cutaneous melanomas presented positivity for COX-2 in 42% of the cases and this expression was associated with positive staining for CD3, CD4, FOXP3 and MAC387. These markers are associated with inflammatory cells, angiogenesis and proliferation. Interestingly, melanomas were highly infiltrated by FOXP3+ cells, this is related to angiogenesis, whereas CD3, CD4 and MAC387 expression was only associated with cutaneous melanomas. The macrophage profile analysis showed that both oral and cutaneous melanomas with low COX-2 expression have an M1 phenoptype, whereas the cases with high COX-2 expression demonstrate a hybrid M1/M2 profile pattern. We concluded that the COX-2 is overexpressed in 42% of cutaneous melanomas and in 34% of oral melanomas, with a direct association with angiogenesis, proliferation, and intratumoral lymphocyte infiltration. We propose that COX-2 is a key regulator of immune cell infiltration and may drive tumour associated macrophage activation. (© 2020 John Wiley & Sons Ltd.) |
| References: | Goldschmidt MH, Hendrick MJ. Tumors of the skin and soft tissues. In: Meuten DJ, ed. Tumors in Domestic Animals. 4th ed. Iowa State, US: Ames; 2002:44-117. Morris J, Dobson J. Skin. In: Morris J, ed. Small Animal Oncology. 1th ed. Oxford, UK: Blackwell Science; 2001:4-67. Vail DM, Withrow SJ. Tumors of the skin and subcutaneous tissues. In: Withrow SJ, Vail DM, eds. Withrow & MacEwen's Small Animal Clinical Oncology. 4th ed. St. Louis, USA: Saunders Elsevier; 2007:375-401. Gross TL, Ihrke PJ, Walder EJ, Affolter VK. Melanocytic Tumours. In: Gross TL, ed. Skin diseases of the dog and cat. 2nd ed. Oxford: Blackwell Science Ltd; 2005:813-836. Scott DW, Miller WH, Griffin CE. Neoplastic and non-neoplastic tumors. In: Muller GH, Kirk RW, eds. Small Animal Dermatology. Vol 2001. 6th ed. Philadelphia: Saunders Company; 2001:1236-1272. Ahmadi M, Emery DC, Morgan DJ. Prevention of both direct and cross-priming of antitumor CD8+ T-cell responses following overproduction of prostaglandin E2 by tumor cells in vivo. Cancer Res. 2008;68(18):7520-7529. Méric JB, Rottey S, Olaussen K, et al. Cyclooxygenase-2 as a target for anticancer drug development. Crit Rev Oncol Hematol. 2006;59(1):51-64. Denkert C, Köbel M, Berger S, et al. Expression of cyclooxygenase 2 in human malignant melanoma. Cancer Res. 2001;61(1):303-308. Greenhough A, Smartt HJ, Moore AE, et al. The COX-2/PGE2 pathway: key roles in the hallmarks of cancer and adaptation to the tumor microenvironment. Carcinogenesis. 2009;30(3):377-386. Doré M. Cyclooxygenase-2 expression in animal cancers. Vet Pathol. 2011 Jan;48(1):254-265. PANG LY, GATENBY EL, et al. Global gene expression analysis of canine osteosarcoma stem cells reveals a novel role for COX-2 in tumor initiation. PLoS One. 2014;9(1):1-13. Kuźbicki L, Sarnecka A, Chwirot B. Expression of cyclooxygenase-2 in benign naevi and during human cutaneous melanoma progression. Melanoma Res. 2006;16(1):29-36. Pires I, Garcia A, Prada J, Queiroga FL. COX-1 e COX-2 expression in canine cutaneous, oral and ocular melanocytic tumors. J Comp Pathol. 2010;143(2-3):142-149. Pockaj BA, Basu GD, Pathangey LB, et al. Reduced T-cell and dendritic cell function is related to cyclooxygenase-2 overexpression and prostaglandin E2 secretion in patients with breast cancer. Ann Surg Oncology. 2004;11(3):328-339. Kirkpatrick K, Ogunkolade W, Elkak A, et al. The mRNA expression of cyclo-oxygenase-2 (COX-2) and vascular endothelial growth factor (VEGF) in human breast cancer. Curr Med Res Opin. 2002;18(4):237-241. Wang D, DuBois RN. The role of COX-2 in intestinal inflammation and colorectal cancer. Oncogene. 2010;29(6):781-788. Esbona K, Saha S, Yi Y, et al. The presence of COX-2 and tumor-associated macrophages as a prognostic marker for invasive breast carcinoma patients. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res. 2016;76(14 Suppl):Abstract no 3927. Monteiro LN, Rodrigues MA, Gomes DA, Salgado BS, Cassali GD. Tumour-associated macrophages: relation with progression and invasiveness, and assessment of M1/M2 macrophages in canine mammary tumor. The Veterinary Journal. 2018;234:119-125. Na YR, Yoon YN, Son DI, Seok SH. Cyclooxygenase-2 inhibition blocks M2 macrophage differentiation and suppresses metastasis in murine breast cancer model. PLoS One. 2013;8(5):e63451. Wang J, Cai D, Ma B, Wu G, Wu J. Skewing the balance of regulatory T-cells and T-helper 17 cells in breast cancer patients. J Int Med Res. 2011;39(3):691-701. Gao YW, Chen YX, Wang ZM, et al. Increased expression of Cyclooxygenase-2 and increased infiltration of regulatory T cells in tumors of patients with hepatocellular carcinoma. Digestion. 2009;79(3):169-176. Gerber AL, Munst A, Schlapbach C, et al. High expression of FOXP3 in primary melanoma is associated with tumor progression. Br J Dermatol. 2014;170(1):103-109. Gregorio H, Raposo TP, Queiroga FL, Prada J, Pires I. Investigating associations of cyclooxygenase-2 expression with angiogenesis, proliferation, macrophage and T-lymphocyte infiltration in canine melanocytic tumors. Melanoma Res. 2016;26(4):338-347. Goldschmidt MH, Dunstan RW, Stannard AA. Melanocytic tumors and tumor like lesions. In: Goldschmidt MH, Dunstan RW, Stannard AA, Tscharner C, Walder EJ, Yager JA, eds. Histological Classification of Epithelial and Melanocytic Tumors of the Skin of Domestic Animals. 2nd ed. Washington, DC: Armed Forces Institute of Pathology, American Registry of Pathology; 1998:38-50. Smedley RC, Spangler WL, Esplin DG, et al. Prognostic markers for canine melanocytic neoplasms: a comparative review of the literature and goals for future investigation. Vet Pathol. 2011;48(1):54-72. Smedley RC, Lamoureux J, Sledge DG, Kiupel M. Immunohistochemical diagnosis of canine Oral Amelanotic melanocytic neoplasms. Vet Pathol. 2011;48(1):32-40. Lavalle GE, Bertagnolli AC, Tavares WLF, Cassali GD. COX-2 expression in canine mammary carcinomas: correlation with angiogenesis and overall survival. Vet Pathol. 2009;46(6):1275-1280. Schor AM, Pendleton N, Pazouki S, et al. Assessment of vascularity in histological sections: effects of methodology and value as an index of angiogenesis in breast tumors. Histochem J. 1998;30(12):849-856. Dutra AP, Azevedo Júnior GM, Schmitt FC, Cassali GD. Assessment of cell proliferation and prognostic factors in canine mammary gland tumors. Arq Bras Med Vet Zootec. 2008;60(6):1403-1412. Carvalho MI, Pires I, Prada J, Queiroga FL. T-lymphocytic infiltrate in canine mammary tumors: clinic and prognostic implications. In Vivo. 2011;25(6):963-969. Hussein MR, Al-Assiri M, Musalam AO. Phenotypic characterization of the infiltrating immune cells in normal prostate, benign nodular prostatic hyperplasia and prostatic adenocarcinoma. Exp Mol Pathol. 2009;86(2):108-113. Jackute J, Zemaitis M, Pranys D, et al. The prognostic influence of tumor infiltrating Foxp3+ CD4+, CD4+ and CD8+ T cells in resected non-small cell lung cancer. J Inflamm. 2015;12:63. Kim JH, Hur JH, Lee SM, Im KS, Kim NH, Sur JH. Correlation of Foxp3 positive regulatory T cells with prognostic factors in canine mammary carcinomas. Veterinary J. 2012;193(1):222-227. Raposo TP, Pires I, Carvalho MI, Prada J, Argyle DJ, Queiroga FL. Tumor-associated macrophages are associated with vascular endothelial growth factor expression in canine mammary tumors. Vet Comp Oncol. 2015;13(4):464-474. Rodrigues MA, Gamba CO, Faria JA, et al. Inner nuclear membrane localization of epidermal growth factor receptor (EGFR) in spontaneous canine model of invasive micropapillary carcinoma of the mammary gland. Pathol Res Pract. 2016;212(2):340-344. Raposo T, Gregório H, Pires I, Prada J, Queiroga FL. Prognostic value of tumourassociated macrophages in canine mammary tumours. Vet Comp Oncol. 2014;12:10-19. Ricklin ME, Roosje P, Summerfield A. Characterization of canine dendritic cells in healthy, atopic, and non-allergic inflamed skin. J Clin Immunol. 2010;30:845-854. Sanches FP, Tomokane TY, Da Matta VL, Marcondes M, Corbett CE, Laurenti MD. Expression of inducible nitric oxide synthase in macrophages inversely correlates with parasitism of lymphoid tissues in dogs with visceral leishmaniasis. Acta Vet Scand. 2014;7:56-57. Weidner N. Measuring intratumoral microvessel density. Methods Enzymol. 2008;444:305-323. Mantovani A, Schioppa T, Porta C, Allavena P, Sica A. Role of tumor-associated macrophages in tumor progression and invasion. Cancer Metastasis Rev. 2006;25(3):315-322. Smith WL, Garavito RM, DeWitt DL. Prostaglandin endoperoxide H synthases (cyclooxygenases)-1 and −2. J Biol Chem. 1996;271(17):33157-33160. Chang AE, Karnell LH, Menck HR. The National Cancer Data Base report on cutaneous and noncutaneous melanoma: a summary of 84,836 cases from the past decade. The American College of Surgeons Commission on Cancer and the American Cancer Society. Cancer. 1998;83(8):1664-1678. Yao L, Liu F, Hong L. The function and mechanism of COX-2 in angiogenesis of gastric cancer cells. J Exp Clin Cancer Res. 2011;30(1):13-17. Ebert LM, MacRaild SE, Zanker D, Davis ID, Cebon J, Chen W. A cancer vaccine induces expansion of NY-ESO-1-specific regulatory T cells in patients with advanced melanoma. PLoS One. 2012;7(10):e48424. Yan X, Jiao SC, Zhang GQ, Guan Y, Wang JL. Tumor-associated immune factors are associated with recurrence and metastasis in non-small cell lung cancer. Cancer Gene Ther. 2017;24(2):57-63. Carter LL. And W Duttont RW. Type 1 and type 2: a fundamental dichotomy for all T-cell subsets. Curr Opin Immunol. 1996;8(3):336-342. Giroux M, Descoteaux A. Cyclooxygenase-2 expression in macrophages: modulation by protein kinase C-α. J Immunol. 2000;165(7):3985-3991. Li H, Yang B, Huang J, et al. Cyclooxygenase-2 in tumor-associated macrophages promotes breast cancer cell survival by triggering a positive-feedback loop between macrophages and cancer cells. Oncotarget. 2015;6(30):29637-29650. Yoshida S, Amano H, Hayashi I. COX-2/VEGF-dependent facilitation of tumor-associated angiogenesis and tumor growth in vivo. Lab Invest. 2003;83(10):1385-1394. Gambichler T, Bindsteiner M, Hoxtermann S, et al. Circulating CD4+ CD25high CD127low regulatory T cells are an independent predictor of advanced melanoma. Pigment Cell Melanoma Res. 2013;26(2):280-283. Coussens LM, Werb Z. Inflammation and cancer. Nature. 2002;420(6917):860-867. Horiuchi Y, Tominaga M, Ichikawa M, et al. Relationship between regulatory and type 1 T cells in dogs with oral malignant melanoma. Microbiol Immunol. 2010;54(3):152-159. |
| معلومات مُعتمدة: | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; Conselho Nacional de Desenvolvimento Científico e Tecnológico |
| فهرسة مساهمة: | Keywords: COX-2; canine melanoma; dog; immunohistochemistry; inflammation; macrophages |
| المشرفين على المادة: | 0 (Biomarkers, Tumor) EC 1.14.99.1 (Cyclooxygenase 2) |
| تواريخ الأحداث: | Date Created: 20200424 Date Completed: 20210908 Latest Revision: 20231213 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1111/vco.12601 |
| PMID: | 32323423 |
| قاعدة البيانات: | MEDLINE |
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