دورية أكاديمية
Long noncoding RNA SNHG12 promotes vascular smooth muscle cell proliferation and migration via regulating miR-199a-5p/HIF-1α.
| العنوان: | Long noncoding RNA SNHG12 promotes vascular smooth muscle cell proliferation and migration via regulating miR-199a-5p/HIF-1α. |
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| المؤلفون: | Sun Y; Department of Vascular Surgery, The First Affiliated Hospital of Jiamusi University, Jiamusi, Heilongjiang, China., Zhao JT; Department of Second Gastroenterology, The First Affiliated Hospital of Jiamusi University, Jiamusi, Heilongjiang, China., Chi BJ; Department of Urology, The First Affiliated Hospital of Jiamusi University, Jiamusi, Heilongjiang, China., Wang KF; Department of Vascular Surgery, The First Affiliated Hospital of Jiamusi University, Jiamusi, Heilongjiang, China. |
| المصدر: | Cell biology international [Cell Biol Int] 2020 Aug; Vol. 44 (8), pp. 1714-1726. Date of Electronic Publication: 2020 May 11. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Chichester Country of Publication: England NLM ID: 9307129 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-8355 (Electronic) Linking ISSN: 10656995 NLM ISO Abbreviation: Cell Biol Int Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2013- : John Wiley & Sons Chichester : Original Publication: London, UK : Published for the International Federation for Cell Biology by Academic Press, c1993- |
| مواضيع طبية MeSH: | Gene Expression Regulation*, Atherosclerosis/*genetics , Hypoxia-Inducible Factor 1, alpha Subunit/*genetics , MicroRNAs/*metabolism , Muscle, Smooth, Vascular/*metabolism , RNA, Long Noncoding/*metabolism, Animals ; Apoptosis ; Atherosclerosis/metabolism ; Carotid Arteries/cytology ; Cell Movement ; Cell Proliferation ; Cells, Cultured ; Hypoxia-Inducible Factor 1, alpha Subunit/metabolism ; Male ; Mice ; Mice, Inbred C57BL ; Muscle, Smooth, Vascular/cytology |
| مستخلص: | The dysregulation of proliferation and migration of vascular smooth muscle cells (VSMCs) contributes to atherosclerosis (AS) and accumulating reports indicate the crucial role of long noncoding RNA in AS. However, the role of small nucleolar RNA host gene 12 (SNHG12) in regulating the phenotypes of VSMCs and AS remains largely unknown. Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) was used to detect the expression levels of SNHG12 and miR-199a-5p in an in vivo AS model and VSMCs treated by oxidized low-density lipoprotein (ox-LDL). The proliferation ability, migration ability, and apoptosis of VSMCs were tested by cell counting kit-8, Transwell assay, and terminal deoxynucleotidyl transferase dUTP nick end labeling assay, respectively. StarBase database was used to predict the binding sites between miR-199a-5p and SNHG12. The interaction between miR-199a-5p and SNHG12 was validated by qRT-PCR, western blot, and luciferase reporter assay. Western blot was used to examine the effects of SNHG12 and miR-199a-5p on the expression of hypoxia-inducible factor 1α (HIF-1α). We found that the expression level of SNHG12 was significantly increased in the animal model and VSMCs treated by ox-LDL. Knockdown of SNHG12 suppressed the proliferation and migration abilities of VSMCs, while overexpression of SNHG12 had the opposite effects. Mechanically, we validated that miR-199a-5p was a target of SNHG12, and the target gene of miR-199a-5p, HIF-1α could be indirectly and positively regulated by SNHG12. In conclusion, SHNG12 targeting miR-199a-5p/HIF-1α contributed to the pathophysiological process of AS by regulating the phenotypes of VSMCs, and could be a potential therapy target for this disease. (© 2020 International Federation for Cell Biology.) |
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| معلومات مُعتمدة: | First Affiliated Hospital of Jiamusi University |
| فهرسة مساهمة: | Keywords: HIF-1α; SHNG12; atherosclerosis; cardiovascular diseases; endogenous competing RNAs; miR-199a-5p |
| المشرفين على المادة: | 0 (Hypoxia-Inducible Factor 1, alpha Subunit) 0 (MicroRNAs) 0 (Mirn199 microRNA, mouse) 0 (RNA, Long Noncoding) 0 (SNHG12 long non-coding RNA, human) 0 (mirn199 microRNA, human) |
| تواريخ الأحداث: | Date Created: 20200428 Date Completed: 20210329 Latest Revision: 20210329 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1002/cbin.11365 |
| PMID: | 32339345 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1095-8355 |
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| DOI: | 10.1002/cbin.11365 |