دورية أكاديمية
A Co-culture Model to Study the Effect of Kidney Fibroblast-p90RSK on Epithelial Cell Survival.
| العنوان: | A Co-culture Model to Study the Effect of Kidney Fibroblast-p90RSK on Epithelial Cell Survival. |
|---|---|
| المؤلفون: | Lin L; Department of Medicine, The Pennsylvania State University College of Medicine, Hershey, PA, USA. llin1@pennstatehealth.psu.edu.; Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, PA, USA. llin1@pennstatehealth.psu.edu., White S; Department of Medicine, The Pennsylvania State University College of Medicine, Hershey, PA, USA.; Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, PA, USA., Hu K; Department of Medicine, The Pennsylvania State University College of Medicine, Hershey, PA, USA. kebinhu@pennstatehealth.psu.edu.; Department of Cellular and Molecular Physiology, The Pennsylvania State University College of Medicine, Hershey, PA, USA. kebinhu@pennstatehealth.psu.edu. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2346, pp. 63-71. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Coculture Techniques*, Epithelial Cells/*metabolism , Fibroblasts/*metabolism , Kidney/*metabolism , Ribosomal Protein S6 Kinases, 90-kDa/*metabolism, Animals ; Cell Survival ; Cells, Cultured ; Humans ; Kidney/cytology ; Mice ; Mice, Transgenic ; Models, Biological |
| مستخلص: | Methods for the mechanistic investigations on renal fibrosis have long been concentrated on individual type of cells, such as fibroblasts and epithelial cells. However, in recent years, growing numbers of studies have been shifting toward the role of the intercellular interactions, such as communication between tubular epithelial cells and fibroblasts. Various co-culture models have been utilized in the studies of cell-cell communication and interaction. In this chapter, we describe an innovative co-culture model employing the porous membranes for spatially partitioning the cells while allowing direct crosstalk between fibroblasts and epithelial cells in an effort of mimicking in vivo environment. |
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| معلومات مُعتمدة: | R01 DK102624 United States DK NIDDK NIH HHS |
| فهرسة مساهمة: | Keywords: Apoptosis; Co-culture; Epithelial cell; In vitro; Primary kidney fibroblast; p90RSK |
| المشرفين على المادة: | EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 90-kDa) |
| تواريخ الأحداث: | Date Created: 20200514 Date Completed: 20210825 Latest Revision: 20220102 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC8429804 |
| DOI: | 10.1007/7651_2020_292 |
| PMID: | 32399746 |
| قاعدة البيانات: | MEDLINE |
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