دورية أكاديمية
Salidroside induces apoptosis and triggers endoplasmic reticulum stress in human hepatocellular carcinoma.
| العنوان: | Salidroside induces apoptosis and triggers endoplasmic reticulum stress in human hepatocellular carcinoma. |
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| المؤلفون: | Ding SY; Department of Hepatobiliary Surgery, Yijishan Hospital, Wannan Medical College, China., Wang MT; Department of Hepatobiliary Surgery, Yijishan Hospital, Wannan Medical College, China., Dai DF; Department of Hepatobiliary Surgery, Yijishan Hospital, Wannan Medical College, China., Peng JL; Department of Hepatobiliary Surgery, Yijishan Hospital, Wannan Medical College, China., Wu WL; Department of Gynecology, Wuhu Maternal and Child Health Family Planning Service Center, China. Electronic address: wuweilin550@163.com. |
| المصدر: | Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2020 Jul 05; Vol. 527 (4), pp. 1057-1063. Date of Electronic Publication: 2020 May 18. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2104 (Electronic) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2002- >: San Diego, CA : Elsevier Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | Antineoplastic Agents, Phytogenic/*pharmacology , Apoptosis/*drug effects , Carcinoma, Hepatocellular/*drug therapy , Endoplasmic Reticulum Stress/*drug effects , Glucosides/*pharmacology , Liver Neoplasms/*drug therapy , Phenols/*pharmacology, Animals ; Antineoplastic Agents, Phytogenic/chemistry ; Antineoplastic Agents, Phytogenic/therapeutic use ; Carcinoma, Hepatocellular/metabolism ; Carcinoma, Hepatocellular/pathology ; Glucosides/chemistry ; Glucosides/therapeutic use ; Hep G2 Cells ; Humans ; Liver Neoplasms/metabolism ; Liver Neoplasms/pathology ; Male ; Mice, Inbred BALB C ; Mice, Nude ; Phenols/chemistry ; Phenols/therapeutic use ; Rhodiola/chemistry |
| مستخلص: | Salidroside possesses excellent anti-tumor activity in many types of malignant tumor. In present study, we focused on the effects of salidroside on hepatocellular carcinoma (HCC). The viability of human HCC cells was assayed using MTT. Apoptosis in the cells and tissues samples were detected by Annexin V/PI or TUNEL staining assays. The levels of apoptosis and endoplasmic reticulum (ER) stress related proteins were measured by western blotting analysis. We found salidroside significantly suppressed cell viability and promoted apoptosis in HCC cells. Salidroside could activate intrinsic and extrinsic apoptotic pathways, by increasing activities of caspase-3, caspase-8 and caspase-9, up-regulating levels of Bax, Cytochrome c and decreasing level of Bcl-2 in HepG2 cells. Moreover, it was found salidroside induced ER stress and increased expression of p-PERK, eIF2a, p-eIF2a, ATF-6 and CHOP in HepG2 cells. Interestingly, knockdown of CHOP impaired salidroside induced inhibitory effects on HepG2 cells, suggesting the important role of ER stress in cytotoxic effect of salidroside. Finally, we have confirmed salidroside induced ER stress and inhibited development of HepG2 in an xenograft mouse model. In conclusion, our data suggest salidroside inhibits viability and induces apoptosis of HCC both in vitro and vivo, and this effect is partially mediated by activation of ER stress. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2020 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Apoptosis; Endoplasmic reticulum stress; Hepatocellular carcinoma; Salidroside |
| المشرفين على المادة: | 0 (Antineoplastic Agents, Phytogenic) 0 (Glucosides) 0 (Phenols) M983H6N1S9 (rhodioloside) |
| تواريخ الأحداث: | Date Created: 20200523 Date Completed: 20210111 Latest Revision: 20210111 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.bbrc.2020.05.066 |
| PMID: | 32439176 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1090-2104 |
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| DOI: | 10.1016/j.bbrc.2020.05.066 |