دورية أكاديمية
Developmental changes induced by exogenous testosterone during early phases of prostate organogenesis.
| العنوان: | Developmental changes induced by exogenous testosterone during early phases of prostate organogenesis. |
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| المؤلفون: | Ramos JG; Department of Histology, Embryology, and Cell Biology, Federal University of Goiás, Av. Esperança, s/n, Campus Samambaia, Goiânia, Goiás 74690-900, Brazil., de Assis Silva JP; Department of Histology, Embryology, and Cell Biology, Federal University of Goiás, Av. Esperança, s/n, Campus Samambaia, Goiânia, Goiás 74690-900, Brazil., Manso LA; Department of Histology, Embryology, and Cell Biology, Federal University of Goiás, Av. Esperança, s/n, Campus Samambaia, Goiânia, Goiás 74690-900, Brazil., Rodrigues GA; Department of Histology, Embryology, and Cell Biology, Federal University of Goiás, Av. Esperança, s/n, Campus Samambaia, Goiânia, Goiás 74690-900, Brazil., Taboga SR; Department of Biology, State University of São Paulo, R. Cristóvão Colombo, 2265, São José do Rio Preto, São Paulo 15054000, Brazil., de Carvalho HF; Department of Structural and Functional Biology, State University of Campinas, Av. Bertrand Russel, s/n, Campinas, São Paulo 13084864, Brazil., Dos Santos FCA; Department of Histology, Embryology, and Cell Biology, Federal University of Goiás, Av. Esperança, s/n, Campus Samambaia, Goiânia, Goiás 74690-900, Brazil., Biancardi MF; Department of Histology, Embryology, and Cell Biology, Federal University of Goiás, Av. Esperança, s/n, Campus Samambaia, Goiânia, Goiás 74690-900, Brazil. Electronic address: manoel_biancardi@ufg.br. |
| المصدر: | Experimental and molecular pathology [Exp Mol Pathol] 2020 Aug; Vol. 115, pp. 104473. Date of Electronic Publication: 2020 May 23. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0370711 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0945 (Electronic) Linking ISSN: 00144800 NLM ISO Abbreviation: Exp Mol Pathol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2000- > : Amsteram : Elsevier Original Publication: New York : Academic Press |
| مواضيع طبية MeSH: | Organogenesis/*drug effects , Prostate/*growth & development , Testosterone/*pharmacology, Animals ; Estrogen Receptor alpha/metabolism ; Female ; Gerbillinae ; Imaging, Three-Dimensional ; Male ; Proliferating Cell Nuclear Antigen/metabolism ; Prostate/anatomy & histology ; Prostate/diagnostic imaging ; Prostate/drug effects ; Receptors, Androgen/metabolism ; Testosterone/blood |
| مستخلص: | The aim of this study was to evaluate the impact of prenatal testosterone exposure on prostate development in male and female neonatal gerbils. Pregnant females were exposed to subcutaneous injections of testosterone cypionate (500 μg/animal) at gestational days 20 and 22. Male and female pups were then euthanized at postnatal day 1. Morphological analysis showed that females were severely affected by androgen exposure. We also observed that male and female urogenital sinus (UGS) responded differentially to testosterone treatment, demonstrating heterogeneous immunostaining for the androgen receptor (AR), estrogen receptor alpha (ERα), and proliferating cell nuclear antigen (PCNA). Smooth muscle α-actin (α-SMA) analysis showed that testosterone delays the myodifferentiation, allowing buds to reach the ectopic mesenchymes of the female UGS. Our data showed that abnormal testosterone exposure disrupted prostate organogenesis, altered the expression patterns of important markers, and demonstrated that female UGS was particularly influenced by androgen exposure during a critical window in the developmental period. (Copyright © 2020 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Development; Gerbils; Masculinization; Prostate; Sexual dimorphism; Testosterone |
| المشرفين على المادة: | 0 (Estrogen Receptor alpha) 0 (Proliferating Cell Nuclear Antigen) 0 (Receptors, Androgen) 3XMK78S47O (Testosterone) |
| تواريخ الأحداث: | Date Created: 20200527 Date Completed: 20201029 Latest Revision: 20201029 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.yexmp.2020.104473 |
| PMID: | 32454105 |
| قاعدة البيانات: | MEDLINE |
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