دورية أكاديمية
Unreliability of EDTA samples for measuring bioamine neurotransmitter levels in cats.
| العنوان: | Unreliability of EDTA samples for measuring bioamine neurotransmitter levels in cats. |
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| المؤلفون: | Ramos D; Department of Medical Clinics, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil., Reche-Junior A; Department of Medical Clinics, Faculty of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil., Brandão JDP; Nephrology Department, Faculty of Medicine, São Paulo Federal University, São Paulo, Brazil., Arita DY; Nephrology Department, Faculty of Medicine, São Paulo Federal University, São Paulo, Brazil., Casarini DE; Nephrology Department, Faculty of Medicine, São Paulo Federal University, São Paulo, Brazil., Mills DS; Animal Behaviour, Cognition and Welfare Group, School of Life Sciences, University of Lincoln, Lincoln, UK. |
| المصدر: | Journal of feline medicine and surgery [J Feline Med Surg] 2021 Feb; Vol. 23 (2), pp. 190-193. Date of Electronic Publication: 2020 Jun 01. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Sage Country of Publication: England NLM ID: 100897329 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-2750 (Electronic) Linking ISSN: 1098612X NLM ISO Abbreviation: J Feline Med Surg Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2012- : London : Sage Original Publication: London ; Philadelphia : WB Saunders Co., |
| مواضيع طبية MeSH: | Neurotransmitter Agents*, Animals ; Cats ; Edetic Acid ; Reproducibility of Results |
| مستخلص: | Objectives: The aim of this study was to assess the reliability of an EDTA-based method for measuring cat blood bioamines. Methods: Test 1 involved collecting blood samples from seven university laboratory cats. The samples were transferred to EDTA, heparin and plain tubes to determine concentrations of four bioamines (serotonin, dopamine, epinephrine [adrenaline] and norepinephrine [noradrenaline]). Correlation of measurements performed on EDTA plasma, with those performed on heparinized plasma or serum were assessed by intraclass correlation coefficients (ICCs). In test 2, blood samples from five owned cats were collected and stored in EDTA tubes and divided between duplicate Eppendorf tubes labeled as different cats for blinding purposes and analyzed independently for the same four bioamines as in test 1. Reliability of concentration determination for these duplicates was assessed by ICCs and coefficients of variation (CVs). Results: In test 1, there was no significant correlation between the EDTA plasma serotonin and serum serotonin concentrations. There was also no significant correlation between EDTA plasma and heparin plasma concentrations for either epinephrine or norepinephrine. There was a statistically significant but weak correlation between EDTA plasma and heparin plasma dopamine concentrations. In test 2, there was no correlation for repeat-analyzed serotonin and epinephrine concentrations. Although there were statistically significant correlations for dopamine and norepinephrine, CVs for each analyte were in excess of 30%. Conclusions and Relevance: Before any further attempt is made to measure and report on neurotransmitter concentrations in domestic cats, it is essential that the robustness of the methodology is carefully validated and the data presented. |
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| فهرسة مساهمة: | Keywords: Chromatography; catecholamines; emotion; serotonin |
| المشرفين على المادة: | 0 (Neurotransmitter Agents) 9G34HU7RV0 (Edetic Acid) |
| تواريخ الأحداث: | Date Created: 20200602 Date Completed: 20211124 Latest Revision: 20240611 |
| رمز التحديث: | 20240611 |
| مُعرف محوري في PubMed: | PMC10741357 |
| DOI: | 10.1177/1098612X20924916 |
| PMID: | 32478638 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1532-2750 |
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| DOI: | 10.1177/1098612X20924916 |