دورية أكاديمية
Single-cell technologies in hepatology: new insights into liver biology and disease pathogenesis.
العنوان: | Single-cell technologies in hepatology: new insights into liver biology and disease pathogenesis. |
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المؤلفون: | Ramachandran P; Centre for Inflammation Research, The Queen's Medical Research Institute, University of Edinburgh, Edinburgh, UK., Matchett KP; Centre for Inflammation Research, The Queen's Medical Research Institute, University of Edinburgh, Edinburgh, UK., Dobie R; Centre for Inflammation Research, The Queen's Medical Research Institute, University of Edinburgh, Edinburgh, UK., Wilson-Kanamori JR; Centre for Inflammation Research, The Queen's Medical Research Institute, University of Edinburgh, Edinburgh, UK., Henderson NC; Centre for Inflammation Research, The Queen's Medical Research Institute, University of Edinburgh, Edinburgh, UK. Neil.Henderson@ed.ac.uk.; MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, UK. Neil.Henderson@ed.ac.uk. |
المصدر: | Nature reviews. Gastroenterology & hepatology [Nat Rev Gastroenterol Hepatol] 2020 Aug; Vol. 17 (8), pp. 457-472. Date of Electronic Publication: 2020 Jun 01. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Review |
اللغة: | English |
بيانات الدورية: | Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101500079 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1759-5053 (Electronic) Linking ISSN: 17595045 NLM ISO Abbreviation: Nat Rev Gastroenterol Hepatol Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: London ; New York : Nature Pub. Group, c2009- |
مواضيع طبية MeSH: | Gene Expression Profiling* , Single-Cell Analysis*, Liver/*metabolism , Liver Diseases/*genetics, Endothelial Cells/metabolism ; Endothelial Cells/physiology ; Fibroblasts/metabolism ; Fibroblasts/physiology ; Gastroenterology ; Hepatic Stellate Cells/metabolism ; Hepatic Stellate Cells/physiology ; Hepatocytes/metabolism ; Hepatocytes/physiology ; Humans ; Inflammation/immunology ; Kupffer Cells/immunology ; Liver/cytology ; Liver/immunology ; Liver/physiology ; Liver Cirrhosis/genetics ; Liver Cirrhosis/immunology ; Liver Cirrhosis/metabolism ; Liver Diseases/immunology ; Liver Diseases/metabolism ; Macrophages/immunology ; Muscle, Smooth, Vascular/cytology ; Myocytes, Smooth Muscle/metabolism ; Myocytes, Smooth Muscle/physiology ; Regeneration ; Sequence Analysis, RNA |
مستخلص: | Liver disease is a major global health-care problem, affecting an estimated 844 million people worldwide. Despite this substantial burden, therapeutic options for liver disease remain limited, in part owing to a paucity of detailed analyses defining the cellular and molecular mechanisms that drive these conditions in humans. Single-cell transcriptomic technologies are transforming our understanding of cellular diversity and function in health and disease. In this Review, we discuss how these technologies have been applied in hepatology, advancing our understanding of cellular heterogeneity and providing novel insights into fundamental liver biology such as the metabolic zonation of hepatocytes, endothelial cells and hepatic stellate cells, and the cellular mechanisms underpinning liver regeneration. Application of these methodologies is also uncovering critical pathophysiological changes driving disease states such as hepatic fibrosis, where distinct populations of macrophages, endothelial cells and mesenchymal cells reside within a spatially distinct fibrotic niche and interact to promote scar formation. In addition, single-cell approaches are starting to dissect key cellular and molecular functions in liver cancer. In the near future, new techniques such as spatial transcriptomics and multiomic approaches will further deepen our understanding of disease pathogenesis, enabling the identification of novel therapeutic targets for patients across the spectrum of liver diseases. |
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معلومات مُعتمدة: | MR/N008340/1 United Kingdom MRC_ Medical Research Council; United Kingdom WT_ Wellcome Trust |
تواريخ الأحداث: | Date Created: 20200603 Date Completed: 20201106 Latest Revision: 20220422 |
رمز التحديث: | 20240628 |
DOI: | 10.1038/s41575-020-0304-x |
PMID: | 32483353 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1759-5053 |
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DOI: | 10.1038/s41575-020-0304-x |