دورية أكاديمية
Functional and Genomic Variation between Human-Derived Isolates of Lachnospiraceae Reveals Inter- and Intra-Species Diversity.
| العنوان: | Functional and Genomic Variation between Human-Derived Isolates of Lachnospiraceae Reveals Inter- and Intra-Species Diversity. |
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| المؤلفون: | Sorbara MT; Duchossois Family Institute, The University of Chicago, Chicago, IL 60637, USA; Department of Microbiology, The University of Chicago, Chicago, IL 60637, USA; Department of Medicine, Section of Infectious Diseases and Global Health, The University of Chicago, Chicago, IL 60637, USA. Electronic address: sorbaram@uchicago.edu., Littmann ER; Duchossois Family Institute, The University of Chicago, Chicago, IL 60637, USA., Fontana E; Center for Microbes, Inflammation and Cancer, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Moody TU; Center for Microbes, Inflammation and Cancer, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Kohout CE; Duchossois Family Institute, The University of Chicago, Chicago, IL 60637, USA., Gjonbalaj M; Center for Microbes, Inflammation and Cancer, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Eaton V; Center for Microbes, Inflammation and Cancer, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Seok R; Center for Microbes, Inflammation and Cancer, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Leiner IM; Duchossois Family Institute, The University of Chicago, Chicago, IL 60637, USA., Pamer EG; Duchossois Family Institute, The University of Chicago, Chicago, IL 60637, USA; Department of Microbiology, The University of Chicago, Chicago, IL 60637, USA; Department of Medicine, Section of Infectious Diseases and Global Health, The University of Chicago, Chicago, IL 60637, USA. Electronic address: egpamer@uchicago.edu. |
| المصدر: | Cell host & microbe [Cell Host Microbe] 2020 Jul 08; Vol. 28 (1), pp. 134-146.e4. Date of Electronic Publication: 2020 Jun 02. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101302316 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1934-6069 (Electronic) Linking ISSN: 19313128 NLM ISO Abbreviation: Cell Host Microbe Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cambridge, Mass. : Cell Press |
| مواضيع طبية MeSH: | Genetic Variation*, Clostridiales/*classification , Clostridiales/*genetics , Gastrointestinal Microbiome/*genetics , Metabolic Networks and Pathways/*genetics, Feces/microbiology ; Genome, Bacterial ; Humans ; Metagenomics ; Phylogeny ; RNA, Ribosomal, 16S/genetics ; Whole Genome Sequencing |
| مستخلص: | Bacteria belonging to the Lachnospiraceae family are abundant, obligate anaerobic members of the microbiota in healthy humans. Lachnospiraceae impact their hosts by producing short-chain fatty acids, converting primary to secondary bile acids, and facilitating colonization resistance against intestinal pathogens. To increase our understanding of genomic and functional diversity between members of this family, we cultured 273 Lachnospiraceae isolates representing 11 genera and 27 species from human donors and performed whole-genome sequencing assembly and annotation. This analysis revealed substantial inter- and intra-species diversity in pathways that likely influence an isolate's ability to impact host health. These differences are likely to impact colonization resistance through lantibiotic expression or intestinal acidification, influence host mucosal immune cells and enterocytes via butyrate production, or contribute to synergism within a consortium by heterogenous polysaccharide metabolism. Identification of these specific functions could facilitate development of probiotic bacterial consortia that drive and/or restore in vivo microbiome functions. Competing Interests: Declaration of Interests E.G.P. has received speaker honoraria from Bristol-Myer Squibb, Celgene, Seres Therapeutics, MedImmune, Novartis, and Ferring Pharmaceuticals; is an inventor on patent application no. WPO2015179437A1, entitled “Methods and compositions for reducing Clostridium difficile infection” and no. WPO2017091753A1, entitled “Methods and compositions for reducing vancomycin-resistant Enterococci infection or colonization”; and holds patents that receive royalties from Seres Therapeutics. The remaining authors declare no competing interests. (Copyright © 2020 Elsevier Inc. All rights reserved.) |
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| معلومات مُعتمدة: | P30 CA008748 United States CA NCI NIH HHS; R01 AI042135 United States AI NIAID NIH HHS; R01 AI095706 United States AI NIAID NIH HHS; U01 AI124275 United States AI NIAID NIH HHS |
| فهرسة مساهمة: | Keywords: Blautia; Clostridia; Lachnospiraceae; Microbiome; butyrate; commensal bacteria; genomic diversity; live biotherapeutics; microbiota; symbiotic bacteria |
| المشرفين على المادة: | 0 (RNA, Ribosomal, 16S) |
| تواريخ الأحداث: | Date Created: 20200604 Date Completed: 20210323 Latest Revision: 20210709 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC7351604 |
| DOI: | 10.1016/j.chom.2020.05.005 |
| PMID: | 32492369 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1934-6069 |
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| DOI: | 10.1016/j.chom.2020.05.005 |